Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii

Detalhes bibliográficos
Autor(a) principal: Araujo, Anna Erika Vieira de
Data de Publicação: 2021
Outros Autores: Conde, Luis Vidal, Silva Junior, Haroldo Cid da, Machado, Lucas de Almeida, Lara, Flavio Alves, Chapeaurouge, Alex, Pauer, Heidi, Hardoim, Cristiane Cassiolato Pires, Antunes, Luis Caetano Martha, Carvalho-Assef, Ana Paula D’Alincourt, Senna, José Procópio Moreno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/46794
Resumo: Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
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spelling Araujo, Anna Erika Vieira deConde, Luis VidalSilva Junior, Haroldo Cid daMachado, Lucas de AlmeidaLara, Flavio AlvesChapeaurouge, AlexPauer, HeidiHardoim, Cristiane Cassiolato PiresAntunes, Luis Caetano MarthaCarvalho-Assef, Ana Paula D’AlincourtSenna, José Procópio Moreno2021-04-20T23:52:14Z2021-04-20T23:52:14Z2021ARAUJO, Anna Erika Vieira de et al. Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii. Microbes, 104801, p. 1-34, Feb. 2021.1769-714Xhttps://www.arca.fiocruz.br/handle/icict/4679410.1016/j.micinf.2021.104801engElsevier Masson SASAcinetobacter baumanniiBamAiImmunogênicoEspecificidadeBactérias resistentesAcinetobacter baumanniiBamAiImmunogenicSpecificityResistant bacteriaCross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumanniiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia da Inovação em Doenças de Populações Negligenciadas. Rio de Janeiro, RJ, Brasil.Universidade de São Paulo. Instituto de Biociências. Coastal Campus de São Vicente, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia da Inovação em Doenças de Populações Negligenciadas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.Acinetobacter baumannii is an important nosocomial pathogen. BamA is a protein that belongs to a complex responsible for organizing the proteins on the bacterial outer membrane. In this work, we aimed to evaluate murine immune responses to BamA recombinant protein (rAbBamA) from A. baumannii in an animal model of infection, and to assess cross-reactivity of this target for the development of anti-A. baumannii vaccines or diagnostics. Immunization of mice with rAbBamA elicited high antibody titers and antibody recognition of native A. baumannii BamA. Immunofluorescence also detected binding to the bacterial surface. After challenge, immunized mice demonstrated a 40% survival increase and better bacterial clearance in kidneys. Immunoblot of anti-rAbBamA against other medically relevant bacteria showed binding to proteins of approximately 35 kDa in Klebsiella pneumoniae and Escherichia coli lysates, primarily identified as OmpA and OmpC, respectively. Altogether, our data show that anti-rAbBamA antibodies provide a protective response against A. baumannii infection in mice. However, the response elicited by immunization with rAbBamA is not completely specific to A. baumannii. Although a broad-spectrum vaccine that protects against various pathogens is an appealing strategy, antibody reactivity against the human microbiota is undesired. In fact, immunization with rAbBamA produced noticeable effects on the gut microbiota. However, the changes elicited were small and non-specific, given that no significant changes in the abundance of Proteobacteria were observed. Overall, rAbBamA is a promising target, but specificity must be considered in the development of immunological tools against A. baumannii.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/46794/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLucasMAchado_FlavioLara_etal_IOC_2021.pdfLucasMAchado_FlavioLara_etal_IOC_2021.pdfapplication/pdf610843https://www.arca.fiocruz.br/bitstream/icict/46794/2/LucasMAchado_FlavioLara_etal_IOC_2021.pdf25a2ebd41d0374d74413fbefdb16d26eMD52TEXTLucasMAchado_FlavioLara_etal_IOC_2021.pdf.txtLucasMAchado_FlavioLara_etal_IOC_2021.pdf.txtExtracted texttext/plain49992https://www.arca.fiocruz.br/bitstream/icict/46794/3/LucasMAchado_FlavioLara_etal_IOC_2021.pdf.txt74754dcc43f0ee6c15613c8180666de0MD53icict/467942023-01-26 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dc.title.pt_BR.fl_str_mv Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii
title Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii
spellingShingle Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii
Araujo, Anna Erika Vieira de
Acinetobacter baumannii
BamA
iImmunogênico
Especificidade
Bactérias resistentes
Acinetobacter baumannii
BamA
iImmunogenic
Specificity
Resistant bacteria
title_short Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii
title_full Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii
title_fullStr Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii
title_full_unstemmed Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii
title_sort Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii
author Araujo, Anna Erika Vieira de
author_facet Araujo, Anna Erika Vieira de
Conde, Luis Vidal
Silva Junior, Haroldo Cid da
Machado, Lucas de Almeida
Lara, Flavio Alves
Chapeaurouge, Alex
Pauer, Heidi
Hardoim, Cristiane Cassiolato Pires
Antunes, Luis Caetano Martha
Carvalho-Assef, Ana Paula D’Alincourt
Senna, José Procópio Moreno
author_role author
author2 Conde, Luis Vidal
Silva Junior, Haroldo Cid da
Machado, Lucas de Almeida
Lara, Flavio Alves
Chapeaurouge, Alex
Pauer, Heidi
Hardoim, Cristiane Cassiolato Pires
Antunes, Luis Caetano Martha
Carvalho-Assef, Ana Paula D’Alincourt
Senna, José Procópio Moreno
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Araujo, Anna Erika Vieira de
Conde, Luis Vidal
Silva Junior, Haroldo Cid da
Machado, Lucas de Almeida
Lara, Flavio Alves
Chapeaurouge, Alex
Pauer, Heidi
Hardoim, Cristiane Cassiolato Pires
Antunes, Luis Caetano Martha
Carvalho-Assef, Ana Paula D’Alincourt
Senna, José Procópio Moreno
dc.subject.other.pt_BR.fl_str_mv Acinetobacter baumannii
BamA
iImmunogênico
Especificidade
Bactérias resistentes
topic Acinetobacter baumannii
BamA
iImmunogênico
Especificidade
Bactérias resistentes
Acinetobacter baumannii
BamA
iImmunogenic
Specificity
Resistant bacteria
dc.subject.en.pt_BR.fl_str_mv Acinetobacter baumannii
BamA
iImmunogenic
Specificity
Resistant bacteria
description Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-04-20T23:52:14Z
dc.date.available.fl_str_mv 2021-04-20T23:52:14Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ARAUJO, Anna Erika Vieira de et al. Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii. Microbes, 104801, p. 1-34, Feb. 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/46794
dc.identifier.issn.pt_BR.fl_str_mv 1769-714X
dc.identifier.doi.none.fl_str_mv 10.1016/j.micinf.2021.104801
identifier_str_mv ARAUJO, Anna Erika Vieira de et al. Cross-reactivity and immunotherapeutic potential of BamA recombinant protein from Acinetobacter baumannii. Microbes, 104801, p. 1-34, Feb. 2021.
1769-714X
10.1016/j.micinf.2021.104801
url https://www.arca.fiocruz.br/handle/icict/46794
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Masson SAS
publisher.none.fl_str_mv Elsevier Masson SAS
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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