Leishmania (L.) amazonensis-induced inhibition of nitric oxide synthesis in host macrophages

Detalhes bibliográficos
Autor(a) principal: Balestieri, Filomena Maria Perrella
Data de Publicação: 2002
Outros Autores: Queiroz, Allan R. Pires, Scavone, Cristoforo, Costa, Vlaudia M. Assis, Barral Netto, Manoel, Abrahamsohn, Ises de Almeida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7437
Resumo: Universidade Federal da Paraíba. Departamento de Fisiologia e Patologia. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil
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spelling Balestieri, Filomena Maria PerrellaQueiroz, Allan R. PiresScavone, CristoforoCosta, Vlaudia M. AssisBarral Netto, ManoelAbrahamsohn, Ises de Almeida2014-03-21T19:19:07Z2014-03-21T19:19:07Z2002BALESTIERI, F. et al. Leishmania (L.) amazonensis-induced inhibition of nitric oxide synthesis in host macrophages. Microbes and Infection, v. 4, p. 23-29, 2002.1286-4579S 1 2 8 6 - 4 5 7 9 ( 0 1 ) 0 1 5 0 5 - 2https://www.arca.fiocruz.br/handle/icict/7437engElsevier SASLeishmania (L.) amazonensis-induced inhibition of nitric oxide synthesis in host macrophagesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal da Paraíba. Departamento de Fisiologia e Patologia. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilUniversidade de São Paulo. ICB. Departamento de Farmacologia. Cidade Universitária. São Paulo, SP, BrasilUniversidade de São Paulo. ICB. Departamento de Imunologia. Cidade Universitária. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilUniversidade de São Paulo. ICB. Departamento de Imunologia. Cidade Universitária. São Paulo, SP, BrasilInhibition of lipopolysaccharide (LPS)-induced nitric oxide (NO) production was demonstrated in J774-G8 macrophages infected with Leishmania (L.) amazonensis promastigotes. The downmodulation of NO production observed in infected and LPS-stimulated J774-G8 cells correlated with a reduction in inducible nitric oxide synthase (iNOS) activity. Reduction in iNOS activity was not paralleled by decreased iNOS mRNA expression, suggesting that the parasite affects post-transcriptional events of NO synthesis. Supplementation with L-arginine or tetrahydrobiopterin did not increase NO production, suggesting that inhibition is not due to an insufficiency of substrate or co-factor. Treatment with anti-IL-10, anti-IL-4 or anti-TGF-beta neutralizing antibodies also failed to increase NO production, indicating that these cytokines are not involved in the observed parasite-induced inhibition of NO synthesis. However, treatment of the cultures with IFN-gamma resulted in a marked increase in NO production by infected LPS-stimulated cells. These results show that although L.(L.) amazonensis infection inhibits iNOS activity and NO production by J774-G8 cells, activation by IFN-gamma is capable of overriding the suppression of NO synthesis.Leishmania (L.) amazonensisMacrophageNitric oxideINOS activityINOS mRNAIFN-γAnimaisCélulas CultivadasLeishmania/patogenicidadeLeishmaniose/parasitologiaMacrófagos/parasitologiaÓxido Nítrico/biossínteseLipopolissacarídeos/farmacologiaMacrófagos/efeitos de drogasMacrófagos/metabolismoCamundongosÓxido Nítrico Sintase/genéticaÓxido Nítrico Sintase/metabolismoÓxido Nítrico Sintase Tipo IIRNA Mensageiro/genéticaRNA Mensageiro/metabolismoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBalestieri F Leishmania (L)....pdfBalestieri F Leishmania (L)....pdfapplication/pdf139505https://www.arca.fiocruz.br/bitstream/icict/7437/1/Balestieri%20F%20Leishmania%20%28L%29....pdf2ba9af398cfaf37b3a7289e2e51be065MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7437/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTBalestieri F Leishmania (L)....pdf.txtBalestieri F Leishmania (L)....pdf.txtExtracted texttext/plain36128https://www.arca.fiocruz.br/bitstream/icict/7437/5/Balestieri%20F%20Leishmania%20%28L%29....pdf.txt66a86acbba613b4cc09e98d948d0ed2dMD55THUMBNAILBalestieri F Leishmania (L)....pdf.jpgBalestieri F Leishmania (L)....pdf.jpgGenerated Thumbnailimage/jpeg1909https://www.arca.fiocruz.br/bitstream/icict/7437/4/Balestieri%20F%20Leishmania%20%28L%29....pdf.jpg635277fd1aaa68e445754074bb0dd751MD54icict/74372023-03-15 14:33:00.549oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Leishmania (L.) amazonensis-induced inhibition of nitric oxide synthesis in host macrophages
title Leishmania (L.) amazonensis-induced inhibition of nitric oxide synthesis in host macrophages
spellingShingle Leishmania (L.) amazonensis-induced inhibition of nitric oxide synthesis in host macrophages
Balestieri, Filomena Maria Perrella
Leishmania (L.) amazonensis
Macrophage
Nitric oxide
INOS activity
INOS mRNA
IFN-γ
Animais
Células Cultivadas
Leishmania/patogenicidade
Leishmaniose/parasitologia
Macrófagos/parasitologia
Óxido Nítrico/biossíntese
Lipopolissacarídeos/farmacologia
Macrófagos/efeitos de drogas
Macrófagos/metabolismo
Camundongos
Óxido Nítrico Sintase/genética
Óxido Nítrico Sintase/metabolismo
Óxido Nítrico Sintase Tipo II
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
title_short Leishmania (L.) amazonensis-induced inhibition of nitric oxide synthesis in host macrophages
title_full Leishmania (L.) amazonensis-induced inhibition of nitric oxide synthesis in host macrophages
title_fullStr Leishmania (L.) amazonensis-induced inhibition of nitric oxide synthesis in host macrophages
title_full_unstemmed Leishmania (L.) amazonensis-induced inhibition of nitric oxide synthesis in host macrophages
title_sort Leishmania (L.) amazonensis-induced inhibition of nitric oxide synthesis in host macrophages
author Balestieri, Filomena Maria Perrella
author_facet Balestieri, Filomena Maria Perrella
Queiroz, Allan R. Pires
Scavone, Cristoforo
Costa, Vlaudia M. Assis
Barral Netto, Manoel
Abrahamsohn, Ises de Almeida
author_role author
author2 Queiroz, Allan R. Pires
Scavone, Cristoforo
Costa, Vlaudia M. Assis
Barral Netto, Manoel
Abrahamsohn, Ises de Almeida
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Balestieri, Filomena Maria Perrella
Queiroz, Allan R. Pires
Scavone, Cristoforo
Costa, Vlaudia M. Assis
Barral Netto, Manoel
Abrahamsohn, Ises de Almeida
dc.subject.en.pt_BR.fl_str_mv Leishmania (L.) amazonensis
Macrophage
Nitric oxide
INOS activity
INOS mRNA
IFN-γ
Animais
Células Cultivadas
topic Leishmania (L.) amazonensis
Macrophage
Nitric oxide
INOS activity
INOS mRNA
IFN-γ
Animais
Células Cultivadas
Leishmania/patogenicidade
Leishmaniose/parasitologia
Macrófagos/parasitologia
Óxido Nítrico/biossíntese
Lipopolissacarídeos/farmacologia
Macrófagos/efeitos de drogas
Macrófagos/metabolismo
Camundongos
Óxido Nítrico Sintase/genética
Óxido Nítrico Sintase/metabolismo
Óxido Nítrico Sintase Tipo II
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
dc.subject.decs.pt_BR.fl_str_mv Leishmania/patogenicidade
Leishmaniose/parasitologia
Macrófagos/parasitologia
Óxido Nítrico/biossíntese
Lipopolissacarídeos/farmacologia
Macrófagos/efeitos de drogas
Macrófagos/metabolismo
Camundongos
Óxido Nítrico Sintase/genética
Óxido Nítrico Sintase/metabolismo
Óxido Nítrico Sintase Tipo II
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
description Universidade Federal da Paraíba. Departamento de Fisiologia e Patologia. Laboratório de Tecnologia Farmacêutica. João Pessoa, PB, Brasil
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2014-03-21T19:19:07Z
dc.date.available.fl_str_mv 2014-03-21T19:19:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BALESTIERI, F. et al. Leishmania (L.) amazonensis-induced inhibition of nitric oxide synthesis in host macrophages. Microbes and Infection, v. 4, p. 23-29, 2002.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7437
dc.identifier.issn.none.fl_str_mv 1286-4579
dc.identifier.other.none.fl_str_mv S 1 2 8 6 - 4 5 7 9 ( 0 1 ) 0 1 5 0 5 - 2
identifier_str_mv BALESTIERI, F. et al. Leishmania (L.) amazonensis-induced inhibition of nitric oxide synthesis in host macrophages. Microbes and Infection, v. 4, p. 23-29, 2002.
1286-4579
S 1 2 8 6 - 4 5 7 9 ( 0 1 ) 0 1 5 0 5 - 2
url https://www.arca.fiocruz.br/handle/icict/7437
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Elsevier SAS
publisher.none.fl_str_mv Elsevier SAS
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instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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