Fonsecaea pedrosoi Sclerotic Cells: Secretion of Aspartic-Type Peptidase and Susceptibility to Peptidase Inhibitors

Detalhes bibliográficos
Autor(a) principal: Palmeira, Vanila F.
Data de Publicação: 2018
Outros Autores: Goulart, Fatima R. V., Granato, Marcela Q., Alviano, Daniela S., Alviano, Celuta S., Kneipp, Lucimar F., Santos, André L. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31298
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Departamento de Microbiologia Geral. Laboratório de Estrutura de Microrganismos. Rio de Janeiro, RJ, Brasil.
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spelling Palmeira, Vanila F.Goulart, Fatima R. V.Granato, Marcela Q.Alviano, Daniela S.Alviano, Celuta S.Kneipp, Lucimar F.Santos, André L. S.2019-01-24T14:51:58Z2019-01-24T14:51:58Z2018PALMEIRA, Vanilla F. et al. Fonsecaea pedrosoi Sclerotic Cells: Secretion of Aspartic-Type Peptidase and Susceptibility to Peptidase Inhibitors. Frontiers in Microbiology, v.9, Article 1383, 9p, June 2018.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/3129810.3389/fmicb.2018.01383engFrontiers MediaFonsecaea pedrosoiDroga antifúngicaPeptidase aspárticoCromoblastomicoseInibidores da peptidaseFonsecaea pedrosoiChromoblastomycosisAspartic peptidasePeptidase inhibitorsAntifungal drugCromoblastomicoseFonsecaea pedrosoi Sclerotic Cells: Secretion of Aspartic-Type Peptidase and Susceptibility to Peptidase Inhibitorsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Departamento de Microbiologia Geral. Laboratório de Estrutura de Microrganismos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Departamento de Microbiologia Geral. Laboratório de Estrutura de Microrganismos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Departamento de Microbiologia Geral. Laboratório de Estrutura de Microrganismos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Departamento de Microbiologia Geral. Laboratório de Estrutura de Microrganismos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Química. Program de Pós-Graduação em Bioquímica. Rio de Janeiro, RJ, Brasil.Fonsecaea pedrosoi is a dematiaceous fungus and the main causative agent of chromoblastomycosis that is a chronic disease usually affecting the human skin and subcutaneous tissues, which causes deformations and incapacities, being frequently refractory to available therapies. A typical globe-shaped, multiseptated and pigmented cells, known as sclerotic cells, are found in the lesions of infected individuals. In the present work, we have investigated the production of aspartic-type peptidase in F. pedrosoi sclerotic cells as well as the effect of peptidase inhibitors (PIs) on its enzymatic activity and viability. Our data showed that sclerotic cells are able to secrete pepstatin A-sensible aspartic peptidase when grown under chemically defined conditions. In addition, aspartic PIs (ritonavir, nelfinavir, indinavir, and saquinavir), which are clinically used in the HIV chemotherapy, significantly decreased the fungal peptidase activity, varying from 55 to 99%. Moreover, sclerotic cell-derived aspartic peptidase hydrolyzed human albumin, an important serum protein, as well as laminin, an extracellular matrix component, but not immunoglobulin G and fibronectin. It is well-known that aspartic peptidases play important physiological roles in fungal cells. With this task in mind, the effect of pepstatin A, a classical aspartic peptidase inhibitor, on the F. pedrosoi proliferation was evaluated. Pepstatin A inhibited the fungal viability in both cellular density- and drug-concentration manners. Moreover, HIV-PIs at 10 μM powerfully inhibited the viability (>65%) of F. pedrosoi sclerotic cells. The detection of aspartic peptidase produced by sclerotic cells, the parasitic form of F. pedrosoi, may contribute to reveal new virulence markers and potential targets for chromoblastomycosis therapy.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31298/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALlucimar_kneipp_etal_IOC_2018.pdflucimar_kneipp_etal_IOC_2018.pdfapplication/pdf2768268https://www.arca.fiocruz.br/bitstream/icict/31298/2/lucimar_kneipp_etal_IOC_2018.pdf6bfaba8269161be4f2fad542533cd21eMD52TEXTlucimar_kneipp_etal_IOC_2018.pdf.txtlucimar_kneipp_etal_IOC_2018.pdf.txtExtracted texttext/plain50539https://www.arca.fiocruz.br/bitstream/icict/31298/3/lucimar_kneipp_etal_IOC_2018.pdf.txt1bdcada4ff8dd802ad8936bc422feed5MD53icict/312982019-01-25 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dc.title.pt_BR.fl_str_mv Fonsecaea pedrosoi Sclerotic Cells: Secretion of Aspartic-Type Peptidase and Susceptibility to Peptidase Inhibitors
title Fonsecaea pedrosoi Sclerotic Cells: Secretion of Aspartic-Type Peptidase and Susceptibility to Peptidase Inhibitors
spellingShingle Fonsecaea pedrosoi Sclerotic Cells: Secretion of Aspartic-Type Peptidase and Susceptibility to Peptidase Inhibitors
Palmeira, Vanila F.
Fonsecaea pedrosoi
Droga antifúngica
Peptidase aspártico
Cromoblastomicose
Inibidores da peptidase
Fonsecaea pedrosoi
Chromoblastomycosis
Aspartic peptidase
Peptidase inhibitors
Antifungal drug
Cromoblastomicose
title_short Fonsecaea pedrosoi Sclerotic Cells: Secretion of Aspartic-Type Peptidase and Susceptibility to Peptidase Inhibitors
title_full Fonsecaea pedrosoi Sclerotic Cells: Secretion of Aspartic-Type Peptidase and Susceptibility to Peptidase Inhibitors
title_fullStr Fonsecaea pedrosoi Sclerotic Cells: Secretion of Aspartic-Type Peptidase and Susceptibility to Peptidase Inhibitors
title_full_unstemmed Fonsecaea pedrosoi Sclerotic Cells: Secretion of Aspartic-Type Peptidase and Susceptibility to Peptidase Inhibitors
title_sort Fonsecaea pedrosoi Sclerotic Cells: Secretion of Aspartic-Type Peptidase and Susceptibility to Peptidase Inhibitors
author Palmeira, Vanila F.
author_facet Palmeira, Vanila F.
Goulart, Fatima R. V.
Granato, Marcela Q.
Alviano, Daniela S.
Alviano, Celuta S.
Kneipp, Lucimar F.
Santos, André L. S.
author_role author
author2 Goulart, Fatima R. V.
Granato, Marcela Q.
Alviano, Daniela S.
Alviano, Celuta S.
Kneipp, Lucimar F.
Santos, André L. S.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Palmeira, Vanila F.
Goulart, Fatima R. V.
Granato, Marcela Q.
Alviano, Daniela S.
Alviano, Celuta S.
Kneipp, Lucimar F.
Santos, André L. S.
dc.subject.other.pt_BR.fl_str_mv Fonsecaea pedrosoi
Droga antifúngica
Peptidase aspártico
Cromoblastomicose
Inibidores da peptidase
topic Fonsecaea pedrosoi
Droga antifúngica
Peptidase aspártico
Cromoblastomicose
Inibidores da peptidase
Fonsecaea pedrosoi
Chromoblastomycosis
Aspartic peptidase
Peptidase inhibitors
Antifungal drug
Cromoblastomicose
dc.subject.en.pt_BR.fl_str_mv Fonsecaea pedrosoi
Chromoblastomycosis
Aspartic peptidase
Peptidase inhibitors
Antifungal drug
dc.subject.decs.pt_BR.fl_str_mv Cromoblastomicose
description Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Departamento de Microbiologia Geral. Laboratório de Estudos Avançados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Departamento de Microbiologia Geral. Laboratório de Estrutura de Microrganismos. Rio de Janeiro, RJ, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-01-24T14:51:58Z
dc.date.available.fl_str_mv 2019-01-24T14:51:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PALMEIRA, Vanilla F. et al. Fonsecaea pedrosoi Sclerotic Cells: Secretion of Aspartic-Type Peptidase and Susceptibility to Peptidase Inhibitors. Frontiers in Microbiology, v.9, Article 1383, 9p, June 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31298
dc.identifier.issn.pt_BR.fl_str_mv 1664-302X
dc.identifier.doi.none.fl_str_mv 10.3389/fmicb.2018.01383
identifier_str_mv PALMEIRA, Vanilla F. et al. Fonsecaea pedrosoi Sclerotic Cells: Secretion of Aspartic-Type Peptidase and Susceptibility to Peptidase Inhibitors. Frontiers in Microbiology, v.9, Article 1383, 9p, June 2018.
1664-302X
10.3389/fmicb.2018.01383
url https://www.arca.fiocruz.br/handle/icict/31298
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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