HIV Aspartic Peptidase Inhibitors Modulate Surface Molecules and Enzyme Activities Involved with Physiopathological Events in Fonsecaea pedrosoi

Detalhes bibliográficos
Autor(a) principal: Palmeira, Vanila F.
Data de Publicação: 2017
Outros Autores: Alviano, Daniela S., Silva, Lys A. Braga, Goulart, Fátima R. V., Granato, Marcela Q., Rozental, Sonia, Alviano, Celuta S., Santos, André L. S., Kneipp, Lucimar F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.3389/fmicb.2017.00918
Texto Completo: https://www.arca.fiocruz.br/handle/icict/24841
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Investigação de Peptidases.. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Departamento de Microbiologia Geral. Laboratório de Estrutura de Microrganismos. Rio de Janeiro, RJ, Brasil.
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spelling Palmeira, Vanila F.Alviano, Daniela S.Silva, Lys A. BragaGoulart, Fátima R. V.Granato, Marcela Q.Rozental, SoniaAlviano, Celuta S.Santos, André L. S.Kneipp, Lucimar F.2018-02-15T16:03:56Z2018-02-15T16:03:56Z2017PALMEIRA, Vanila F. et al. HIV Aspartic Peptidase Inhibitors Modulate Surface Molecules and Enzyme Activities Involved with Physiopathological Events in Fonsecaea pedrosoi. Frontiers in Microbiology, v.8, Article 918, 12p, May 2017.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/2484110.3389/fmicb.2017.00918engFrontiers MediaCromoblastomicosePeptídeo Hidrolasesação antifúngicaInibidores de peptidase aspártica por HIVchromoblastomycosisFonsecaea pedrosoipeptidasesHIV aspartic peptidase inhibitorsantifungal actionHIV Aspartic Peptidase Inhibitors Modulate Surface Molecules and Enzyme Activities Involved with Physiopathological Events in Fonsecaea pedrosoiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Investigação de Peptidases.. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Departamento de Microbiologia Geral. Laboratório de Estrutura de Microrganismos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Microbiologia Geral. Laboratório de Estrutura de Microrganismos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Investigação de Peptidases.. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro,. Instituto de Química. Programa de Pós-Graduação em Bioquímica. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Departamento de Microbiologia Geral. Laboratório de Estrutura de Microrganismos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Biologia Celular de Fungos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Microbiologia Geral. Laboratório de Estrutura de Microrganismos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Investigação de Peptidases.. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro,. Instituto de Química. Programa de Pós-Graduação em Bioquímica. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Bioprospecção de Fungos. Rio de Janeiro, RJ. Brasil.Fonsecaea pedrosoi is the main etiological agent of chromoblastomycosis, a recalcitrant disease that is extremely difficult to treat. Therefore, new chemotherapeutics to combat this fungal infection are urgently needed. Although aspartic peptidase inhibitors (PIs) currently used in the treatment of human immunodeficiency virus (HIV) have shown anti-F. pedrosoiactivity their exact mechanisms of action have not been elucidated. In the present study, we have investigated the effects of four HIV-PIs on crucial virulence attributes expressed byF. pedrosoiconidial cells, including surface molecules and secreted enzymes, both of which are directly involved in the disease development. In all the experiments, conidia were treated with indinavir, nelfinavir, ritonavir and saquinavir (100 μM) for 24 h, and then fungal cells were used to evaluate the effects of HIV-PIs on different virulence attributes expressed byF. pedrosoi. In comparison to untreated controls, exposure ofF. pedrosoicells to HIV-PIs caused (i) reduction on the conidial granularity; (ii) irreversible surface ultrastructural alterations, such as shedding of electron dense and amorphous material from the cell wall, undulations/invaginations of the plasma membrane with and withdrawal of this membrane from the cell wall; (iii) a decrease in both mannose-rich glycoconjugates and melanin molecules and an increase in glucosylceramides on the conidial surface; (iv) inhibition of ergosterol and lanosterol production; (v) reduction in the secretion of aspartic peptidase, esterase and phospholipase; (vi) significant reduction in the viability of non-pigmented conidia compared to pigmented ones. In summary, HIV-PIs are efficient drugs with an ability to block crucial biological processes ofF. pedrosoiand can be seriously considered as potential compounds for the development of new chromoblastomycosis chemotherapeutics.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24841/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmarcela_granato_etal_IOC_2017.pdfmarcela_granato_etal_IOC_2017.pdfapplication/pdf5852523https://www.arca.fiocruz.br/bitstream/icict/24841/2/marcela_granato_etal_IOC_2017.pdfe846ccae79cad3efe38a0666b4104396MD52TEXTmarcela_granato_etal_IOC_2017.pdf.txtmarcela_granato_etal_IOC_2017.pdf.txtExtracted texttext/plain57433https://www.arca.fiocruz.br/bitstream/icict/24841/3/marcela_granato_etal_IOC_2017.pdf.txt1fc3c68831dd61cc40e387c27889c9e0MD53icict/248412023-09-05 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dc.title.pt_BR.fl_str_mv HIV Aspartic Peptidase Inhibitors Modulate Surface Molecules and Enzyme Activities Involved with Physiopathological Events in Fonsecaea pedrosoi
title HIV Aspartic Peptidase Inhibitors Modulate Surface Molecules and Enzyme Activities Involved with Physiopathological Events in Fonsecaea pedrosoi
spellingShingle HIV Aspartic Peptidase Inhibitors Modulate Surface Molecules and Enzyme Activities Involved with Physiopathological Events in Fonsecaea pedrosoi
Palmeira, Vanila F.
Cromoblastomicose
Peptídeo Hidrolases
ação antifúngica
Inibidores de peptidase aspártica por HIV
chromoblastomycosis
Fonsecaea pedrosoi
peptidases
HIV aspartic peptidase inhibitors
antifungal action
title_short HIV Aspartic Peptidase Inhibitors Modulate Surface Molecules and Enzyme Activities Involved with Physiopathological Events in Fonsecaea pedrosoi
title_full HIV Aspartic Peptidase Inhibitors Modulate Surface Molecules and Enzyme Activities Involved with Physiopathological Events in Fonsecaea pedrosoi
title_fullStr HIV Aspartic Peptidase Inhibitors Modulate Surface Molecules and Enzyme Activities Involved with Physiopathological Events in Fonsecaea pedrosoi
title_full_unstemmed HIV Aspartic Peptidase Inhibitors Modulate Surface Molecules and Enzyme Activities Involved with Physiopathological Events in Fonsecaea pedrosoi
title_sort HIV Aspartic Peptidase Inhibitors Modulate Surface Molecules and Enzyme Activities Involved with Physiopathological Events in Fonsecaea pedrosoi
author Palmeira, Vanila F.
author_facet Palmeira, Vanila F.
Alviano, Daniela S.
Silva, Lys A. Braga
Goulart, Fátima R. V.
Granato, Marcela Q.
Rozental, Sonia
Alviano, Celuta S.
Santos, André L. S.
Kneipp, Lucimar F.
author_role author
author2 Alviano, Daniela S.
Silva, Lys A. Braga
Goulart, Fátima R. V.
Granato, Marcela Q.
Rozental, Sonia
Alviano, Celuta S.
Santos, André L. S.
Kneipp, Lucimar F.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Palmeira, Vanila F.
Alviano, Daniela S.
Silva, Lys A. Braga
Goulart, Fátima R. V.
Granato, Marcela Q.
Rozental, Sonia
Alviano, Celuta S.
Santos, André L. S.
Kneipp, Lucimar F.
dc.subject.other.pt_BR.fl_str_mv Cromoblastomicose
Peptídeo Hidrolases
ação antifúngica
Inibidores de peptidase aspártica por HIV
topic Cromoblastomicose
Peptídeo Hidrolases
ação antifúngica
Inibidores de peptidase aspártica por HIV
chromoblastomycosis
Fonsecaea pedrosoi
peptidases
HIV aspartic peptidase inhibitors
antifungal action
dc.subject.en.pt_BR.fl_str_mv chromoblastomycosis
Fonsecaea pedrosoi
peptidases
HIV aspartic peptidase inhibitors
antifungal action
description Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Investigação de Peptidases.. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Departamento de Microbiologia Geral. Laboratório de Estrutura de Microrganismos. Rio de Janeiro, RJ, Brasil.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-02-15T16:03:56Z
dc.date.available.fl_str_mv 2018-02-15T16:03:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PALMEIRA, Vanila F. et al. HIV Aspartic Peptidase Inhibitors Modulate Surface Molecules and Enzyme Activities Involved with Physiopathological Events in Fonsecaea pedrosoi. Frontiers in Microbiology, v.8, Article 918, 12p, May 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/24841
dc.identifier.issn.pt_BR.fl_str_mv 1664-302X
dc.identifier.doi.none.fl_str_mv 10.3389/fmicb.2017.00918
identifier_str_mv PALMEIRA, Vanila F. et al. HIV Aspartic Peptidase Inhibitors Modulate Surface Molecules and Enzyme Activities Involved with Physiopathological Events in Fonsecaea pedrosoi. Frontiers in Microbiology, v.8, Article 918, 12p, May 2017.
1664-302X
10.3389/fmicb.2017.00918
url https://www.arca.fiocruz.br/handle/icict/24841
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/24841/2/marcela_granato_etal_IOC_2017.pdf
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