Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Rigato, Paula Ordonhez
Data de Publicação: 2011
Outros Autores: Bargieri, Bruna Cunha de Alencar, Vasconcelos, Jose Ronnie Carvalho de, Dominguez, Mariana Ribeiro, Araujo, Adriano Fernando, Machado, Alexandre Vieira, Gazzinelli, Ricardo Tostes, Romero, Oscar Bruna, Rodrigues, Mauricio Martins
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9029
Resumo: Universidade Federal de São Paulo. Centro de Terapia Celular e Molecular. São Paulo, SP, Brasil /Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
id CRUZ_6bc56681f0884fda5d1d63302cc0cc86
oai_identifier_str oai:www.arca.fiocruz.br:icict/9029
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Rigato, Paula OrdonhezBargieri, Bruna Cunha de AlencarVasconcelos, Jose Ronnie Carvalho deDominguez, Mariana RibeiroAraujo, Adriano FernandoMachado, Alexandre VieiraGazzinelli, Ricardo TostesRomero, Oscar BrunaRodrigues, Mauricio Martins2014-12-02T13:27:20Z2014-12-02T13:27:20Z20110019-9567https://www.arca.fiocruz.br/handle/icict/9029engAmerican Society for MicrobiologyHeterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Centro de Terapia Celular e Molecular. São Paulo, SP, Brasil /Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrasilUniversidade Federal de São Paulo. Centro de Terapia Celular e Molecular. São Paulo, SP, Brasil /Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrasilUniversidade Federal de São Paulo. Centro de Terapia Celular e Molecular. São Paulo, SP, Brasil /Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrasilUniversidade Federal de São Paulo. Centro de Terapia Celular e Molecular. São Paulo, SP, Brasil /Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrasilUniversidade Federal de São Paulo. Centro de Terapia Celular e Molecular. São Paulo, SP, Brasil /Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil/University of Massachusetts Medical School. Department of Medicine. Division of Infectious Disease and Immunology. Worcester, MA, USAUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, BrasilUniversidade Federal de São Paulo. Centro de Terapia Celular e Molecular/ Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, BrasilRIGATO, Paula Ordonhez et al. Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi. Infect Immun, v. 79, n. 5, p. 2120-2130, 2011.Recently, we described a heterologous prime-boost strategy using plasmid DNA followed by replication defective human recombinant adenovirus type 5 as a powerful strategy to elicit long-lived CD8 T-cellmediated protective immunity against experimental systemic infection of mice with a human intracellular protozoan parasite, Trypanosoma cruzi. In the present study, we further characterized the protective long-lived CD8 T cells. We compared several functional and phenotypic aspects of specific CD8 T cells present 14 or 98 days after the last immunizing dose and found the following: (i) the numbers of specific cells were similar, as determined by multimer staining or by determining the number of gamma interferon (IFN-)-secreting cells by enzyme-linked immunospot (ELISPOT) assay; (ii) these cells were equally cytotoxic in vivo; (iii) following in vitro stimulation, a slight decline in the frequency of multifunctional cells (CD107a IFN- or CD107a IFN- tumor necrosis factor alpha positive [TNF-]) was paralleled by a significant increase of CD107a singly positive cells after 98 days; (iv) the expression of several surface markers was identical, except for the reexpression of CD127 after 98 days; (v) the use of genetically deficient mice revealed a role for interleukin-12 (IL-12)/IL-23, but not IFN-, in the maintenance of these memory cells; and (vi) subsequent immunizations with an unrelated virus or a plasmid vaccine or the depletion of CD4 T cells did not significantly erode the number or function of these CD8 T cells during the 15-week period. From these results, we concluded that heterologous plasmid DNA prime-adenovirus boost vaccination generated a stable pool of functional protective long-lived CD8 T cells with an effector memory phenotype.heterologousTrypanosoma cruziinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9029/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALHeterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi.pdfHeterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi.pdfapplication/pdf2398637https://www.arca.fiocruz.br/bitstream/icict/9029/2/Heterologous%20plasmid%20DNA%20prime-recombinant%20human%20adenovirus%205%20boost%20vaccination%20generates%20a%20stable%20pool%20of%20protective%20long-lived%20CD8%28%2b%29%20T%20effector%20memory%20cells%20specific%20for%20a%20human%20parasite%2c%20Trypanosoma%20cruzi.pdfe9232344aacc0a4d2a9f97ba98a21e84MD52TEXTHeterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi.pdf.txtHeterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi.pdf.txtExtracted texttext/plain64538https://www.arca.fiocruz.br/bitstream/icict/9029/3/Heterologous%20plasmid%20DNA%20prime-recombinant%20human%20adenovirus%205%20boost%20vaccination%20generates%20a%20stable%20pool%20of%20protective%20long-lived%20CD8%28%2b%29%20T%20effector%20memory%20cells%20specific%20for%20a%20human%20parasite%2c%20Trypanosoma%20cruzi.pdf.txt97b33dd805dbf9cdbc4b9fe993bbcccbMD53icict/90292019-06-19 10:13:24.364oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:13:24Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi
title Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi
spellingShingle Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi
Rigato, Paula Ordonhez
heterologous
Trypanosoma cruzi
title_short Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi
title_full Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi
title_fullStr Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi
title_full_unstemmed Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi
title_sort Heterologous plasmid DNA prime-recombinant human adenovirus 5 boost vaccination generates a stable pool of protective long-lived CD8(+) T effector memory cells specific for a human parasite, Trypanosoma cruzi
author Rigato, Paula Ordonhez
author_facet Rigato, Paula Ordonhez
Bargieri, Bruna Cunha de Alencar
Vasconcelos, Jose Ronnie Carvalho de
Dominguez, Mariana Ribeiro
Araujo, Adriano Fernando
Machado, Alexandre Vieira
Gazzinelli, Ricardo Tostes
Romero, Oscar Bruna
Rodrigues, Mauricio Martins
author_role author
author2 Bargieri, Bruna Cunha de Alencar
Vasconcelos, Jose Ronnie Carvalho de
Dominguez, Mariana Ribeiro
Araujo, Adriano Fernando
Machado, Alexandre Vieira
Gazzinelli, Ricardo Tostes
Romero, Oscar Bruna
Rodrigues, Mauricio Martins
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rigato, Paula Ordonhez
Bargieri, Bruna Cunha de Alencar
Vasconcelos, Jose Ronnie Carvalho de
Dominguez, Mariana Ribeiro
Araujo, Adriano Fernando
Machado, Alexandre Vieira
Gazzinelli, Ricardo Tostes
Romero, Oscar Bruna
Rodrigues, Mauricio Martins
dc.subject.en.pt_BR.fl_str_mv heterologous
Trypanosoma cruzi
topic heterologous
Trypanosoma cruzi
description Universidade Federal de São Paulo. Centro de Terapia Celular e Molecular. São Paulo, SP, Brasil /Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-12-02T13:27:20Z
dc.date.available.fl_str_mv 2014-12-02T13:27:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9029
dc.identifier.issn.none.fl_str_mv 0019-9567
identifier_str_mv 0019-9567
url https://www.arca.fiocruz.br/handle/icict/9029
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/9029/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/9029/2/Heterologous%20plasmid%20DNA%20prime-recombinant%20human%20adenovirus%205%20boost%20vaccination%20generates%20a%20stable%20pool%20of%20protective%20long-lived%20CD8%28%2b%29%20T%20effector%20memory%20cells%20specific%20for%20a%20human%20parasite%2c%20Trypanosoma%20cruzi.pdf
https://www.arca.fiocruz.br/bitstream/icict/9029/3/Heterologous%20plasmid%20DNA%20prime-recombinant%20human%20adenovirus%205%20boost%20vaccination%20generates%20a%20stable%20pool%20of%20protective%20long-lived%20CD8%28%2b%29%20T%20effector%20memory%20cells%20specific%20for%20a%20human%20parasite%2c%20Trypanosoma%20cruzi.pdf.txt
bitstream.checksum.fl_str_mv 7d48279ffeed55da8dfe2f8e81f3b81f
e9232344aacc0a4d2a9f97ba98a21e84
97b33dd805dbf9cdbc4b9fe993bbcccb
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798325091416670208

Registros relacionados