Comparative Elisa reagents for detection of hepatitis B surface atingen (HBsAg)

Detalhes bibliográficos
Autor(a) principal: Camargo, I. F.
Data de Publicação: 1987
Outros Autores: Gaspar, Ana Maria, Yoshida, Clara F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/42876
Resumo: Conjugados de IgG anti-HBs de cabra e peroxidase, preparados por dois métodos diferentes, um usando NaIO4 e o outro SPDP foram comparados. Anticorpos anti-HBs obtidos de cabra, coelho e cobaio foram testados como captura. O ELISA apresentou sensibilidade similar ao RIA e o nível de captação antigênica foi de 4,37 a 8,75 nanogramas/ml quando utilizados captura de coelho ou cobaio combinados tanto com o conjugado pelo NaIO4 como com o conjugado pelo SPDP.
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spelling Camargo, I. F.Gaspar, Ana MariaYoshida, Clara F.2020-08-22T14:14:23Z2020-08-22T14:14:23Z1987CAMARGO, I. F.; GASPAR, A. M. C.; YOSHIDA, C. F. Comparative Elisa reagents for detection of Hepatitis B surface Antigen (HBsAg). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 82, n. 2, p. 181-187, abr./jun. 1987.0074-0276https://www.arca.fiocruz.br/handle/icict/4287610.1590/s0074-027619870002000051678-8060Conjugados de IgG anti-HBs de cabra e peroxidase, preparados por dois métodos diferentes, um usando NaIO4 e o outro SPDP foram comparados. Anticorpos anti-HBs obtidos de cabra, coelho e cobaio foram testados como captura. O ELISA apresentou sensibilidade similar ao RIA e o nível de captação antigênica foi de 4,37 a 8,75 nanogramas/ml quando utilizados captura de coelho ou cobaio combinados tanto com o conjugado pelo NaIO4 como com o conjugado pelo SPDP.Conjugates of goat anti-HBs IgG and horseradish peroxidase (HRP) prepared by two different methods, one using NaIO4 and the other SPDP, were compared. Anti-HBs antibodies obtained from goat, rabbit and guinea-pig were tested as capture serum. The ELISA showed a sensitivity similar to RIA and a level of antigen captation ranging from 4.37 to 8.75 nanograms/ml was obtained when rabbit or guinea-pig captures were used combined with both NaIO4 or SPDP conjugates.Fundação Oswaldo Cruz. Bio-Manguinhos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.engFundação Oswaldo Cruz. 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dc.title.pt_BR.fl_str_mv Comparative Elisa reagents for detection of hepatitis B surface atingen (HBsAg)
title Comparative Elisa reagents for detection of hepatitis B surface atingen (HBsAg)
spellingShingle Comparative Elisa reagents for detection of hepatitis B surface atingen (HBsAg)
Camargo, I. F.
ELISA
Reagentes
Anticorpos anti-HB
ELISA
IgG anti-HBs
Hepatitis B surface antigen
title_short Comparative Elisa reagents for detection of hepatitis B surface atingen (HBsAg)
title_full Comparative Elisa reagents for detection of hepatitis B surface atingen (HBsAg)
title_fullStr Comparative Elisa reagents for detection of hepatitis B surface atingen (HBsAg)
title_full_unstemmed Comparative Elisa reagents for detection of hepatitis B surface atingen (HBsAg)
title_sort Comparative Elisa reagents for detection of hepatitis B surface atingen (HBsAg)
author Camargo, I. F.
author_facet Camargo, I. F.
Gaspar, Ana Maria
Yoshida, Clara F.
author_role author
author2 Gaspar, Ana Maria
Yoshida, Clara F.
author2_role author
author
dc.contributor.author.fl_str_mv Camargo, I. F.
Gaspar, Ana Maria
Yoshida, Clara F.
dc.subject.other.pt_BR.fl_str_mv ELISA
Reagentes
Anticorpos anti-HB
topic ELISA
Reagentes
Anticorpos anti-HB
ELISA
IgG anti-HBs
Hepatitis B surface antigen
dc.subject.en.pt_BR.fl_str_mv ELISA
IgG anti-HBs
Hepatitis B surface antigen
description Conjugados de IgG anti-HBs de cabra e peroxidase, preparados por dois métodos diferentes, um usando NaIO4 e o outro SPDP foram comparados. Anticorpos anti-HBs obtidos de cabra, coelho e cobaio foram testados como captura. O ELISA apresentou sensibilidade similar ao RIA e o nível de captação antigênica foi de 4,37 a 8,75 nanogramas/ml quando utilizados captura de coelho ou cobaio combinados tanto com o conjugado pelo NaIO4 como com o conjugado pelo SPDP.
publishDate 1987
dc.date.issued.fl_str_mv 1987
dc.date.accessioned.fl_str_mv 2020-08-22T14:14:23Z
dc.date.available.fl_str_mv 2020-08-22T14:14:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CAMARGO, I. F.; GASPAR, A. M. C.; YOSHIDA, C. F. Comparative Elisa reagents for detection of Hepatitis B surface Antigen (HBsAg). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 82, n. 2, p. 181-187, abr./jun. 1987.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/42876
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/s0074-02761987000200005
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv CAMARGO, I. F.; GASPAR, A. M. C.; YOSHIDA, C. F. Comparative Elisa reagents for detection of Hepatitis B surface Antigen (HBsAg). Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 82, n. 2, p. 181-187, abr./jun. 1987.
0074-0276
10.1590/s0074-02761987000200005
1678-8060
url https://www.arca.fiocruz.br/handle/icict/42876
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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