Comparative ELISA reagents for detection of hepatitis B surface antigen (HBsAg)
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 1987 |
| Outros Autores: | , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Memórias do Instituto Oswaldo Cruz |
| Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761987000200005 |
Resumo: | Conjugates of goat anti-HBs IgG and horseradish peroxidase (HRP) prepared by two different methods, one using NaIO4 and the other SPDP, were compared. Anti-HBs antibodies obtained from goat, rabbit and guinea-pig were tested as capture serum. The ELISA showed a sensitivity similar to RIA and a level of antigen captation ranging from 4.37 to 8.75 nanograms/ml was obtained when rabbit or guinea-pig captures were used combined with both NaIO4 or SPDP conjugates. |
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Memórias do Instituto Oswaldo Cruz |
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Comparative ELISA reagents for detection of hepatitis B surface antigen (HBsAg)ElisaHBsAgConjugates of goat anti-HBs IgG and horseradish peroxidase (HRP) prepared by two different methods, one using NaIO4 and the other SPDP, were compared. Anti-HBs antibodies obtained from goat, rabbit and guinea-pig were tested as capture serum. The ELISA showed a sensitivity similar to RIA and a level of antigen captation ranging from 4.37 to 8.75 nanograms/ml was obtained when rabbit or guinea-pig captures were used combined with both NaIO4 or SPDP conjugates.Instituto Oswaldo Cruz, Ministério da Saúde1987-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761987000200005Memórias do Instituto Oswaldo Cruz v.82 n.2 1987reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761987000200005info:eu-repo/semantics/openAccessCamargo,I. F.Gaspar,A. M. C.Yoshida,C. F. T.eng2020-04-25T17:45:52Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:01:41.269Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
| dc.title.none.fl_str_mv |
Comparative ELISA reagents for detection of hepatitis B surface antigen (HBsAg) |
| title |
Comparative ELISA reagents for detection of hepatitis B surface antigen (HBsAg) |
| spellingShingle |
Comparative ELISA reagents for detection of hepatitis B surface antigen (HBsAg) Camargo,I. F. Elisa HBsAg |
| title_short |
Comparative ELISA reagents for detection of hepatitis B surface antigen (HBsAg) |
| title_full |
Comparative ELISA reagents for detection of hepatitis B surface antigen (HBsAg) |
| title_fullStr |
Comparative ELISA reagents for detection of hepatitis B surface antigen (HBsAg) |
| title_full_unstemmed |
Comparative ELISA reagents for detection of hepatitis B surface antigen (HBsAg) |
| title_sort |
Comparative ELISA reagents for detection of hepatitis B surface antigen (HBsAg) |
| author |
Camargo,I. F. |
| author_facet |
Camargo,I. F. Gaspar,A. M. C. Yoshida,C. F. T. |
| author_role |
author |
| author2 |
Gaspar,A. M. C. Yoshida,C. F. T. |
| author2_role |
author author |
| dc.contributor.author.fl_str_mv |
Camargo,I. F. Gaspar,A. M. C. Yoshida,C. F. T. |
| dc.subject.por.fl_str_mv |
Elisa HBsAg |
| topic |
Elisa HBsAg |
| dc.description.none.fl_txt_mv |
Conjugates of goat anti-HBs IgG and horseradish peroxidase (HRP) prepared by two different methods, one using NaIO4 and the other SPDP, were compared. Anti-HBs antibodies obtained from goat, rabbit and guinea-pig were tested as capture serum. The ELISA showed a sensitivity similar to RIA and a level of antigen captation ranging from 4.37 to 8.75 nanograms/ml was obtained when rabbit or guinea-pig captures were used combined with both NaIO4 or SPDP conjugates. |
| description |
Conjugates of goat anti-HBs IgG and horseradish peroxidase (HRP) prepared by two different methods, one using NaIO4 and the other SPDP, were compared. Anti-HBs antibodies obtained from goat, rabbit and guinea-pig were tested as capture serum. The ELISA showed a sensitivity similar to RIA and a level of antigen captation ranging from 4.37 to 8.75 nanograms/ml was obtained when rabbit or guinea-pig captures were used combined with both NaIO4 or SPDP conjugates. |
| publishDate |
1987 |
| dc.date.none.fl_str_mv |
1987-06-01 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761987000200005 |
| url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761987000200005 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
10.1590/S0074-02761987000200005 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
text/html |
| dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
| publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
| dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.82 n.2 1987 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
| reponame_str |
Memórias do Instituto Oswaldo Cruz |
| collection |
Memórias do Instituto Oswaldo Cruz |
| instname_str |
Fundação Oswaldo Cruz |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
| repository.mail.fl_str_mv |
|
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1669937648332963840 |