HIV-1 Tat protein enhances the intracellular growth of Leishmania amazonensis via the ds-RNA induced protein PKR

Detalhes bibliográficos
Autor(a) principal: Vivarini, Aislan de Carvalho
Data de Publicação: 2015
Outros Autores: Pereira, Renata de Meirelles Santos, Souza, Victor Barreto de, Temerozo, Jairo Ramos, Soares, Deivid C., Saraiva, Elvira M., Saliba, Alessandra Mattos, Bou-Habib, Dumith Chequer, Lopes, Ulisses Gazos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13529
Resumo: Universidade Federal do Rio Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Vivarini, Aislan de CarvalhoPereira, Renata de Meirelles SantosSouza, Victor Barreto deTemerozo, Jairo RamosSoares, Deivid C.Saraiva, Elvira M.Saliba, Alessandra MattosBou-Habib, Dumith ChequerLopes, Ulisses Gazos2016-04-05T13:55:25Z2016-04-05T13:55:25Z2015VIVARINI, Aislan de Carvalho; et al. HIV-1 Tat protein enhances the intracellular growth of Leishmania amazonensis via the ds-RNA induced protein PKR. Scientific Reports, v.5:16777, 11p, Nov. 2015.2045-2322https://www.arca.fiocruz.br/handle/icict/1352910.1038/srep16777engNature Publishing GroupHIV-1 Tat protein enhances the intracellular growth of Leishmania amazonensis via the ds-RNA induced protein PKRinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Janeiro. Instituto de Microbiologia Paulo Góes. Laboratório de Imunobiologia de Leishmanioses. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Janeiro. Instituto de Microbiologia Paulo Góes. Laboratório de Imunobiologia de Leishmanioses. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.HIV-1 co-infection with human parasitic diseases is a growing public health problem worldwide. Leishmania parasites infect and replicate inside macrophages, thereby subverting host signaling pathways, including the response mediated by PKR. The HIV-1 Tat protein interacts with PKR and plays a pivotal role in HIV-1 replication. This study shows that Tat increases both the expression and activation of PKR in Leishmania-infected macrophages. Importantly, the positive effect of Tat addition on parasite growth was dependent on PKR signaling, as demonstrated in PKR-deficient macrophages or macrophages treated with the PKR inhibitor. The effect of HIV-1 Tat on parasite growth was prevented when the supernatant of HIV-1-infected macrophages was treated with neutralizing anti-HIV-1 Tat prior to Leishmania infection. The addition of HIV-1 Tat to Leishmania-infected macrophages led to inhibition of iNOS expression, modulation of NF-kB activation and enhancement of IL-10 expression. Accordingly, the expression of a Tat construct containing mutations in the basic region (49-57aa), which is responsible for the interaction with PKR, favored neither parasite growth nor IL-10 expression in infected macrophages. In summary, we show that Tat enhances Leishmania growth through PKR signaling.HIV-1Tat proteinLeishmania amazonensisprotein PKRds-RNAHIV-1Produtos do Gene tatLeishmania braziliensiseIF-2 Quinaseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13529/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjairo_temerozo_etal_IOC_2015.pdfjairo_temerozo_etal_IOC_2015.pdfapplication/pdf1066333https://www.arca.fiocruz.br/bitstream/icict/13529/2/jairo_temerozo_etal_IOC_2015.pdf1894875d5be6eb5dea98e3f11ac0ce98MD52TEXTjairo_temerozo_etal_IOC_2015.pdf.txtjairo_temerozo_etal_IOC_2015.pdf.txtExtracted texttext/plain48896https://www.arca.fiocruz.br/bitstream/icict/13529/3/jairo_temerozo_etal_IOC_2015.pdf.txt33dc24f01ea05b62bf93494d349a0ca9MD53icict/135292018-04-02 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dc.title.pt_BR.fl_str_mv HIV-1 Tat protein enhances the intracellular growth of Leishmania amazonensis via the ds-RNA induced protein PKR
title HIV-1 Tat protein enhances the intracellular growth of Leishmania amazonensis via the ds-RNA induced protein PKR
spellingShingle HIV-1 Tat protein enhances the intracellular growth of Leishmania amazonensis via the ds-RNA induced protein PKR
Vivarini, Aislan de Carvalho
HIV-1
Tat protein
Leishmania amazonensis
protein PKR
ds-RNA
HIV-1
Produtos do Gene tat
Leishmania braziliensis
eIF-2 Quinase
title_short HIV-1 Tat protein enhances the intracellular growth of Leishmania amazonensis via the ds-RNA induced protein PKR
title_full HIV-1 Tat protein enhances the intracellular growth of Leishmania amazonensis via the ds-RNA induced protein PKR
title_fullStr HIV-1 Tat protein enhances the intracellular growth of Leishmania amazonensis via the ds-RNA induced protein PKR
title_full_unstemmed HIV-1 Tat protein enhances the intracellular growth of Leishmania amazonensis via the ds-RNA induced protein PKR
title_sort HIV-1 Tat protein enhances the intracellular growth of Leishmania amazonensis via the ds-RNA induced protein PKR
author Vivarini, Aislan de Carvalho
author_facet Vivarini, Aislan de Carvalho
Pereira, Renata de Meirelles Santos
Souza, Victor Barreto de
Temerozo, Jairo Ramos
Soares, Deivid C.
Saraiva, Elvira M.
Saliba, Alessandra Mattos
Bou-Habib, Dumith Chequer
Lopes, Ulisses Gazos
author_role author
author2 Pereira, Renata de Meirelles Santos
Souza, Victor Barreto de
Temerozo, Jairo Ramos
Soares, Deivid C.
Saraiva, Elvira M.
Saliba, Alessandra Mattos
Bou-Habib, Dumith Chequer
Lopes, Ulisses Gazos
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vivarini, Aislan de Carvalho
Pereira, Renata de Meirelles Santos
Souza, Victor Barreto de
Temerozo, Jairo Ramos
Soares, Deivid C.
Saraiva, Elvira M.
Saliba, Alessandra Mattos
Bou-Habib, Dumith Chequer
Lopes, Ulisses Gazos
dc.subject.en.pt_BR.fl_str_mv HIV-1
Tat protein
Leishmania amazonensis
protein PKR
ds-RNA
topic HIV-1
Tat protein
Leishmania amazonensis
protein PKR
ds-RNA
HIV-1
Produtos do Gene tat
Leishmania braziliensis
eIF-2 Quinase
dc.subject.decs.pt_BR.fl_str_mv HIV-1
Produtos do Gene tat
Leishmania braziliensis
eIF-2 Quinase
description Universidade Federal do Rio Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-04-05T13:55:25Z
dc.date.available.fl_str_mv 2016-04-05T13:55:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VIVARINI, Aislan de Carvalho; et al. HIV-1 Tat protein enhances the intracellular growth of Leishmania amazonensis via the ds-RNA induced protein PKR. Scientific Reports, v.5:16777, 11p, Nov. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13529
dc.identifier.issn.none.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/srep16777
identifier_str_mv VIVARINI, Aislan de Carvalho; et al. HIV-1 Tat protein enhances the intracellular growth of Leishmania amazonensis via the ds-RNA induced protein PKR. Scientific Reports, v.5:16777, 11p, Nov. 2015.
2045-2322
10.1038/srep16777
url https://www.arca.fiocruz.br/handle/icict/13529
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/13529/2/jairo_temerozo_etal_IOC_2015.pdf
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