IL-27 enhances Leishmania amazonensis infection via ds-RNAdependent kinase (PKR) and IL-10 signaling

Detalhes bibliográficos
Autor(a) principal: Souza, Victor Barreto de
Data de Publicação: 2015
Outros Autores: Ferreira, Pedro L. C., Vivarini, Aislan de Carvalho, Silva, Teresa Calegari, Soares, Deivid Costa, Regis, Eduardo G., Pereira, Renata M. S., Silva, Aristóbolo M., Saraiva, Elvira M., Lopes, Ulisses G., Bou-Habib, Dumith Chequer
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12091
Resumo: 2016-03-31
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spelling Souza, Victor Barreto deFerreira, Pedro L. C.Vivarini, Aislan de CarvalhoSilva, Teresa CalegariSoares, Deivid CostaRegis, Eduardo G.Pereira, Renata M. S.Silva, Aristóbolo M.Saraiva, Elvira M.Lopes, Ulisses G.Bou-Habib, Dumith Chequer2015-10-27T12:32:46Z2016-04-01T07:30:09Z2015SOUZA, Victor Barreto de; et al. IL-27 enhances Leishmania amazonensis infection via ds-RNAdependent kinase (PKR) and IL-10 signaling. Immunobiology, v.220, n.4, p.437–444, Apr. 2015.0171-2985https://www.arca.fiocruz.br/handle/icict/1209110.1016/j.imbio.2014.11.006engElsevierIL-27 enhances Leishmania amazonensis infection via ds-RNAdependent kinase (PKR) and IL-10 signalinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2016-03-31Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Imunologia. Instituto de Microbiologia Paulo de Góes. Laboratório de Imunobiologia de Leishmaniose. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Genes Inflamatórios. Belo Horizonte, MG, Brasil.Universidade Federal do Rio de Janeiro. Departamento de Imunologia. Instituto de Microbiologia Paulo de Góes. Laboratório de Imunobiologia de Leishmaniose. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia. Transnacional Pesquisa em Saúde e Meio Ambiente na Região Amazônica (INCT/IMPeTAM). Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.tThe protozoan parasite Leishmania infects and replicates in macrophages, causing a spectrum of diseasesin the human host, varying from cutaneous to visceral clinical forms. It is known that cytokines modulatethe immunological response against Leishmania and are relevant for infection resolution. Here, we reportthat Interleukin (IL)-27 increases Leishmania amazonensis replication in human and murine macrophagesand that the blockage of the IL-10 receptor on the surface of infected cells abolished the IL-27-mediatedenhancement of Leishmania growth. IL-27 induced the activation/phosphorylation of protein kinase R(PKR) in macrophages, and PKR blockage or PKR gene deletion abrogated the enhancement of the parasitegrowth driven by IL-27, as well as the L. amazonensis-induced macrophage production of IL-27. We alsoobserved that L. amazonensis-induced expression of IL-27 depends on type I interferon signaling and theengagement of Toll-like receptor 2. Treatment of Leishmania-infected mice with IL-27 increased lesionsize and parasite loads in the footpad and lymph nodes of infected animals, indicating that this cytokineexerts a local and a systemic effect on parasite growth and propagation. In conclusion, we show thatIL-27 is a L. amazonensis-enhancing factor and that the PKR/IFN1 axis and IL-10 are critical mediators ofthis IL-27 induced effect.IL-27IL-10LeishmaniaMacrophagesPKRLeishmania amazonensisLeishmaniaMacrófagosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/12091/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALvitor_souza_etal_IOC_2015.pdfvitor_souza_etal_IOC_2015.pdfapplication/pdf1152074https://www.arca.fiocruz.br/bitstream/icict/12091/2/vitor_souza_etal_IOC_2015.pdfe7631d176c55775a0431747e9b74e70aMD52TEXTvitor_souza_etal_IOC_2015.pdf.txtvitor_souza_etal_IOC_2015.pdf.txtExtracted texttext/plain47739https://www.arca.fiocruz.br/bitstream/icict/12091/3/vitor_souza_etal_IOC_2015.pdf.txtbf286000b18a942f8560e91a8c9743bfMD53icict/120912023-09-05 10:36:29.193oai:www.arca.fiocruz.br:icict/12091Q0VTU8ODTyBOw4NPLUVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcyBhdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKQ0VERSBlIFRSQU5TRkVSRSwgdG90YWwgZSBncmF0dWl0YW1lbnRlLCDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVosIGVtIGNhcsOhdGVyIHBlcm1hbmVudGUsIGlycmV2b2fDoXZlbCBlIE7Dg08gRVhDTFVTSVZPLCAKdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08gQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcyAKZGUgdm96IGUgaW1hZ2VtIHZpbmN1bGFkb3Mgw6AgT0JSQSwgZHVyYW50ZSB0b2RvIG8gcHJhem8gZGUgZHVyYcOnw6NvIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgZW0gcXVhbHF1ZXIgaWRpb21hIGUgZW0gdG9kb3Mgb3MgcGHDrXNlczsKICAgICAgICAKQUNFSVRBIHF1ZSBhIGNlc3PDo28gdG90YWwgbsOjbyBleGNsdXNpdmEsIHBlcm1hbmVudGUgZSBpcnJldm9nw6F2ZWwgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIHBhdHJpbW9uaWFpcyBuw6NvIGNvbWVyY2lhaXMgZGUgdXRpbGl6YcOnw6NvIApkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwgb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciAKbWVpbyBvdSB2ZcOtY3VsbywgaW5jbHVzaXZlIGVtIFJlcG9zaXTDs3Jpb3MgRGlnaXRhaXMsIGJlbSBjb21vIG9zIGRpcmVpdG9zIGRlIHJlcHJvZHXDp8OjbywgZXhpYmnDp8OjbywgZXhlY3XDp8OjbywgZGVjbGFtYcOnw6NvLCBleHBvc2nDp8OjbywgCmFycXVpdmFtZW50bywgaW5jbHVzw6NvIGVtIGJhbmNvIGRlIGRhZG9zLCBwcmVzZXJ2YcOnw6NvLCBkaWZ1c8OjbywgZGlzdHJpYnVpw6fDo28sIGRpdnVsZ2HDp8OjbywgZW1wcsOpc3RpbW8sIHRyYWR1w6fDo28sIGluY2x1c8OjbyBlbSBub3ZhcyAKb2JyYXMgb3UgY29sZXTDom5lYXMsIHJldXRpbGl6YcOnw6NvLCBlZGnDp8OjbywgcHJvZHXDp8OjbyBkZSBtYXRlcmlhbCBkaWTDoXRpY28gZSBjdXJzb3Mgb3UgcXVhbHF1ZXIgZm9ybWEgZGUgdXRpbGl6YcOnw6NvIG7Do28gY29tZXJjaWFsOwogICAgICAgIApSRUNPTkhFQ0UgcXVlIGEgY2Vzc8OjbyBhcXVpIGVzcGVjaWZpY2FkYSBjb25jZWRlIMOgIEZJT0NSVVogLSBGVU5EQcOHw4NPIE9TV0FMRE8gQ1JVWiBvIGRpcmVpdG8gZGUgYXV0b3JpemFyIHF1YWxxdWVyIHBlc3NvYSDigJMgZsOtc2ljYSAKb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdSBlc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlciAKZmluYWxpZGFkZXMgbsOjbyBjb21lcmNpYWlzOwogICAgICAgIApERUNMQVJBIHF1ZSBhIG9icmEgw6kgY3JpYcOnw6NvIG9yaWdpbmFsIGUgcXVlIMOpIG8gdGl0dWxhciBkb3MgZGlyZWl0b3MgYXF1aSBjZWRpZG9zIGUgYXV0b3JpemFkb3MsIHJlc3BvbnNhYmlsaXphbmRvLXNlIGludGVncmFsbWVudGUgCnBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwgaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIAppbmRlbml6YXIgdGVyY2Vpcm9zIHBvciBkYW5vcywgYmVtIGNvbW8gaW5kZW5pemFyIGUgcmVzc2FyY2lyIMOgIEZJT0NSVVogLSBGVU5EQcOHw4NPIE9TV0FMRE8gQ1JVWiBkZSBldmVudHVhaXMgZGVzcGVzYXMgcXVlIHZpZXJlbSBhIApzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91IGRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIAplIHZpb2xhw6fDtWVzIGRlIGRpcmVpdG9zOwogICAgICAgIApBRklSTUEgcXVlIGNvbmhlY2UgYSBQb2zDrXRpY2EgSW5zdGl0dWNpb25hbCBkZSBBY2Vzc28gQWJlcnRvIGRhIEluc3RpdHVpw6fDo28gZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyAKaW5zdGl0dWNpb25hbCBBUkNBLgo=Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-05T13:36:29Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv IL-27 enhances Leishmania amazonensis infection via ds-RNAdependent kinase (PKR) and IL-10 signaling
title IL-27 enhances Leishmania amazonensis infection via ds-RNAdependent kinase (PKR) and IL-10 signaling
spellingShingle IL-27 enhances Leishmania amazonensis infection via ds-RNAdependent kinase (PKR) and IL-10 signaling
Souza, Victor Barreto de
IL-27
IL-10
Leishmania
Macrophages
PKR
Leishmania amazonensis
Leishmania
Macrófagos
title_short IL-27 enhances Leishmania amazonensis infection via ds-RNAdependent kinase (PKR) and IL-10 signaling
title_full IL-27 enhances Leishmania amazonensis infection via ds-RNAdependent kinase (PKR) and IL-10 signaling
title_fullStr IL-27 enhances Leishmania amazonensis infection via ds-RNAdependent kinase (PKR) and IL-10 signaling
title_full_unstemmed IL-27 enhances Leishmania amazonensis infection via ds-RNAdependent kinase (PKR) and IL-10 signaling
title_sort IL-27 enhances Leishmania amazonensis infection via ds-RNAdependent kinase (PKR) and IL-10 signaling
author Souza, Victor Barreto de
author_facet Souza, Victor Barreto de
Ferreira, Pedro L. C.
Vivarini, Aislan de Carvalho
Silva, Teresa Calegari
Soares, Deivid Costa
Regis, Eduardo G.
Pereira, Renata M. S.
Silva, Aristóbolo M.
Saraiva, Elvira M.
Lopes, Ulisses G.
Bou-Habib, Dumith Chequer
author_role author
author2 Ferreira, Pedro L. C.
Vivarini, Aislan de Carvalho
Silva, Teresa Calegari
Soares, Deivid Costa
Regis, Eduardo G.
Pereira, Renata M. S.
Silva, Aristóbolo M.
Saraiva, Elvira M.
Lopes, Ulisses G.
Bou-Habib, Dumith Chequer
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Victor Barreto de
Ferreira, Pedro L. C.
Vivarini, Aislan de Carvalho
Silva, Teresa Calegari
Soares, Deivid Costa
Regis, Eduardo G.
Pereira, Renata M. S.
Silva, Aristóbolo M.
Saraiva, Elvira M.
Lopes, Ulisses G.
Bou-Habib, Dumith Chequer
dc.subject.en.pt_BR.fl_str_mv IL-27
IL-10
Leishmania
Macrophages
PKR
Leishmania amazonensis
topic IL-27
IL-10
Leishmania
Macrophages
PKR
Leishmania amazonensis
Leishmania
Macrófagos
dc.subject.decs.pt_BR.fl_str_mv Leishmania
Macrófagos
description 2016-03-31
publishDate 2015
dc.date.accessioned.fl_str_mv 2015-10-27T12:32:46Z
dc.date.issued.fl_str_mv 2015
dc.date.available.fl_str_mv 2016-04-01T07:30:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA, Victor Barreto de; et al. IL-27 enhances Leishmania amazonensis infection via ds-RNAdependent kinase (PKR) and IL-10 signaling. Immunobiology, v.220, n.4, p.437–444, Apr. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12091
dc.identifier.issn.none.fl_str_mv 0171-2985
dc.identifier.doi.none.fl_str_mv 10.1016/j.imbio.2014.11.006
identifier_str_mv SOUZA, Victor Barreto de; et al. IL-27 enhances Leishmania amazonensis infection via ds-RNAdependent kinase (PKR) and IL-10 signaling. Immunobiology, v.220, n.4, p.437–444, Apr. 2015.
0171-2985
10.1016/j.imbio.2014.11.006
url https://www.arca.fiocruz.br/handle/icict/12091
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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