Long non-coding RNAs associated with ribosomes in human adipose-derived stem cells: from RNAs to microproteins

Detalhes bibliográficos
Autor(a) principal: Bonilauri, Bernardo
Data de Publicação: 2021
Outros Autores: Holetz, Fabíola Barbieri, Dallagiovanna, Bruno
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50365
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
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spelling Bonilauri, BernardoHoletz, Fabíola BarbieriDallagiovanna, Bruno2021-12-13T19:08:03Z2021-12-13T19:08:03Z2021BONILAURI, Bernardo et al. Long non-coding RNAs associated with ribosomes in human adipose-derived stem cells: From RNAs to microproteins. Biomolecules, v.11, n. 1673, p. 1–17, 2021.2218-273Xhttps://www.arca.fiocruz.br/handle/icict/5036510.3390/biom11111673porMDPIMicroproteínalncRNARNA, Long NoncodingRibosomesOpen Reading FramesMicroproteinProtein BiosynthesisStem CellsARN Largo no CodificanteRibosomasSistemas de Lectura AbiertaBiosíntesis de ProteínasCélulas MadreARN long non codantRibosomesCadres ouverts de lectureBiosynthèse des protéinesCellules souchesRNA Longo não CodificanteRibossomosFases de Leitura AbertaBiossíntese de ProteínasCélulas-TroncoLong non-coding RNAs associated with ribosomes in human adipose-derived stem cells: from RNAs to microproteinsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Ribosome profiling reveals the translational dynamics of mRNAs by capturing a ribosomal footprint snapshot. Growing evidence shows that several long non-coding RNAs (lncRNAs) contain small open reading frames (smORFs) that are translated into functional peptides. The difficulty in identifying bona-fide translated smORFs is a constant challenge in experimental and bioinformatics fields due to their unconventional characteristics. This motivated us to isolate human adipose-derived stem cells (hASC) from adipose tissue and perform a ribosome profiling followed by bioinformatics analysis of transcriptome, translatome, and ribosome-protected fragments of lncRNAs. Here, we demonstrated that 222 lncRNAs were associated with the translational machinery in hASC, including the already demonstrated lncRNAs coding microproteins. The ribosomal occupancy of some transcripts was consistent with the translation of smORFs. In conclusion, we were able to identify a subset of 15 lncRNAs containing 35 smORFs that likely encode functional microproteins, including four previously demonstrated smORF-derived microproteins, suggesting a possible dual role of these lncRNAs in hASC self-renewal.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/50365/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALbiomolecules-11-01673OK.pdfbiomolecules-11-01673OK.pdfapplication/pdf2857284https://www.arca.fiocruz.br/bitstream/icict/50365/2/biomolecules-11-01673OK.pdf62f96d35528d955b48653e4bc416fe3aMD52icict/503652022-12-12 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dc.title.pt_BR.fl_str_mv Long non-coding RNAs associated with ribosomes in human adipose-derived stem cells: from RNAs to microproteins
title Long non-coding RNAs associated with ribosomes in human adipose-derived stem cells: from RNAs to microproteins
spellingShingle Long non-coding RNAs associated with ribosomes in human adipose-derived stem cells: from RNAs to microproteins
Bonilauri, Bernardo
Microproteína
lncRNA
RNA, Long Noncoding
Ribosomes
Open Reading Frames
Microprotein
Protein Biosynthesis
Stem Cells
ARN Largo no Codificante
Ribosomas
Sistemas de Lectura Abierta
Biosíntesis de Proteínas
Células Madre
ARN long non codant
Ribosomes
Cadres ouverts de lecture
Biosynthèse des protéines
Cellules souches
RNA Longo não Codificante
Ribossomos
Fases de Leitura Aberta
Biossíntese de Proteínas
Células-Tronco
title_short Long non-coding RNAs associated with ribosomes in human adipose-derived stem cells: from RNAs to microproteins
title_full Long non-coding RNAs associated with ribosomes in human adipose-derived stem cells: from RNAs to microproteins
title_fullStr Long non-coding RNAs associated with ribosomes in human adipose-derived stem cells: from RNAs to microproteins
title_full_unstemmed Long non-coding RNAs associated with ribosomes in human adipose-derived stem cells: from RNAs to microproteins
title_sort Long non-coding RNAs associated with ribosomes in human adipose-derived stem cells: from RNAs to microproteins
author Bonilauri, Bernardo
author_facet Bonilauri, Bernardo
Holetz, Fabíola Barbieri
Dallagiovanna, Bruno
author_role author
author2 Holetz, Fabíola Barbieri
Dallagiovanna, Bruno
author2_role author
author
dc.contributor.author.fl_str_mv Bonilauri, Bernardo
Holetz, Fabíola Barbieri
Dallagiovanna, Bruno
dc.subject.other.pt_BR.fl_str_mv Microproteína
topic Microproteína
lncRNA
RNA, Long Noncoding
Ribosomes
Open Reading Frames
Microprotein
Protein Biosynthesis
Stem Cells
ARN Largo no Codificante
Ribosomas
Sistemas de Lectura Abierta
Biosíntesis de Proteínas
Células Madre
ARN long non codant
Ribosomes
Cadres ouverts de lecture
Biosynthèse des protéines
Cellules souches
RNA Longo não Codificante
Ribossomos
Fases de Leitura Aberta
Biossíntese de Proteínas
Células-Tronco
dc.subject.en.pt_BR.fl_str_mv lncRNA
RNA, Long Noncoding
Ribosomes
Open Reading Frames
Microprotein
Protein Biosynthesis
Stem Cells
dc.subject.es.pt_BR.fl_str_mv ARN Largo no Codificante
Ribosomas
Sistemas de Lectura Abierta
Biosíntesis de Proteínas
Células Madre
dc.subject.fr.pt_BR.fl_str_mv ARN long non codant
Ribosomes
Cadres ouverts de lecture
Biosynthèse des protéines
Cellules souches
dc.subject.decs.pt_BR.fl_str_mv RNA Longo não Codificante
Ribossomos
Fases de Leitura Aberta
Biossíntese de Proteínas
Células-Tronco
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-12-13T19:08:03Z
dc.date.available.fl_str_mv 2021-12-13T19:08:03Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BONILAURI, Bernardo et al. Long non-coding RNAs associated with ribosomes in human adipose-derived stem cells: From RNAs to microproteins. Biomolecules, v.11, n. 1673, p. 1–17, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50365
dc.identifier.issn.pt_BR.fl_str_mv 2218-273X
dc.identifier.doi.none.fl_str_mv 10.3390/biom11111673
identifier_str_mv BONILAURI, Bernardo et al. Long non-coding RNAs associated with ribosomes in human adipose-derived stem cells: From RNAs to microproteins. Biomolecules, v.11, n. 1673, p. 1–17, 2021.
2218-273X
10.3390/biom11111673
url https://www.arca.fiocruz.br/handle/icict/50365
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50365/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50365/2/biomolecules-11-01673OK.pdf
bitstream.checksum.fl_str_mv 783568c2893d2e25a99990b126be1772
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bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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