Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil

Detalhes bibliográficos
Autor(a) principal: Rodríguez-Arellanes, Gabriela
Data de Publicação: 2013
Outros Autores: Sousa, Carolina Nascimento de, Medeiros-Muniz, Mauro de, Ramírez, José A., Pizzini, Cláudia V., Abreu-Almeida, Marcos de, Oliveira, Manoel M. Evangelista de, Fusco-Almeida, Ana-Marisa, Vite-Garín, Tania, Pitangui, Nayla S., Estrada-Bárcenas, Daniel A., González-González, Antonio E., Mendes-Giannini, María José Soares, Zancopé-Oliveira, Rosely M., Taylor, Maria-Lucia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35677
Resumo: 2020-03-19
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spelling Rodríguez-Arellanes, GabrielaSousa, Carolina Nascimento deMedeiros-Muniz, Mauro deRamírez, José A.Pizzini, Cláudia V.Abreu-Almeida, Marcos deOliveira, Manoel M. Evangelista deFusco-Almeida, Ana-MarisaVite-Garín, TaniaPitangui, Nayla S.Estrada-Bárcenas, Daniel A.González-González, Antonio E.Mendes-Giannini, María José SoaresZancopé-Oliveira, Rosely M.Taylor, Maria-Lucia2019-09-19T13:24:06Z2019-09-19T13:24:06Z2013RODRÍGUEZ-ARELLANA, Gabriela et al. Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil. Eukaryotic Cell, v. 12, n. 7, p. 1033-1038, 2013.1535-9778https://www.arca.fiocruz.br/handle/icict/3567710.1128/EC.00012-131535-9786engAmerican Society for MicrobiologyFrequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-03-19Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. México DF, Mexico.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. México DF, Mexico.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Araraquara, SP, Brasil.Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. México DF, Mexico.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Araraquara, SP, Brasil.Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Colección Nacional de Cultivos Microbianos. México DF, Mexico.Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. México DF, Mexico.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Araraquara, SP, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. México DF, Mexico.The MAT1-1 and MAT1-2 idiomorphs associated with the MAT1 locus of Histoplasma capsulatum were identified by PCR. A total of 28 fungal isolates, 6 isolates from human clinical samples and 22 isolates from environmental (infected bat and contaminated soil) samples, were studied. Among the 14 isolates from Mexico, 71.4% (95% confidence interval [95% CI], 48.3% to 94.5%) were of the MAT1-2 genotype, whereas 100% of the isolates from Brazil were of the MAT1-1 genotype. Each MAT1 idiomorphic region was sequenced and aligned, using the sequences of the G-217B (+ mating type) and G-186AR (- mating type) strains as references. BLASTn analyses of the MAT1-1 and MAT1-2 sequences studied correlated with their respective + and - mating type genotypes. Trees were generated by the maximum likelihood (ML) method to search for similarity among isolates of each MAT1 idiomorph. All MAT1-1 isolates originated from Brazilian bats formed a well-defined group; three isolates from Mexico, the G-217B strain, and a subgroup encompassing all soil-derived isolates and two clinical isolates from Brazil formed a second group; last, one isolate (EH-696P) from a migratory bat captured in Mexico formed a third group of the MAT1-1 genotype. The MAT1-2 idiomorph formed two groups, one of which included two H. capsulatum isolates from infected bats that were closely related to the G-186AR strain. The other group was formed by two human isolates and six isolates from infected bats. Concatenated ML trees, with internal transcribed spacer 1 (ITS1) -5.8S-ITS2 and MAT1-1 or MAT1-2 sequences, support the relatedness of MAT1-1 or MAT1-2 isolates. H. capsulatum mating types were associated with the geographical origin of the isolates, and all isolates from Brazil correlated with their environmental sources.Histoplasma capsulatumMAT1MexicoBrazilinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/35677/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_ Rodríguez-Arellanes_Gabriela_etal_INI_2013.pdfve_ Rodríguez-Arellanes_Gabriela_etal_INI_2013.pdfapplication/pdf443208https://www.arca.fiocruz.br/bitstream/icict/35677/2/ve_%20Rodr%c3%adguez-Arellanes_Gabriela_etal_INI_2013.pdf2008e9d7a51af037f6035bce06556548MD52TEXTve_ Rodríguez-Arellanes_Gabriela_etal_INI_2013.pdf.txtve_ Rodríguez-Arellanes_Gabriela_etal_INI_2013.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil
title Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil
spellingShingle Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil
Rodríguez-Arellanes, Gabriela
Histoplasma capsulatum
MAT1
Mexico
Brazil
title_short Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil
title_full Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil
title_fullStr Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil
title_full_unstemmed Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil
title_sort Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil
author Rodríguez-Arellanes, Gabriela
author_facet Rodríguez-Arellanes, Gabriela
Sousa, Carolina Nascimento de
Medeiros-Muniz, Mauro de
Ramírez, José A.
Pizzini, Cláudia V.
Abreu-Almeida, Marcos de
Oliveira, Manoel M. Evangelista de
Fusco-Almeida, Ana-Marisa
Vite-Garín, Tania
Pitangui, Nayla S.
Estrada-Bárcenas, Daniel A.
González-González, Antonio E.
Mendes-Giannini, María José Soares
Zancopé-Oliveira, Rosely M.
Taylor, Maria-Lucia
author_role author
author2 Sousa, Carolina Nascimento de
Medeiros-Muniz, Mauro de
Ramírez, José A.
Pizzini, Cláudia V.
Abreu-Almeida, Marcos de
Oliveira, Manoel M. Evangelista de
Fusco-Almeida, Ana-Marisa
Vite-Garín, Tania
Pitangui, Nayla S.
Estrada-Bárcenas, Daniel A.
González-González, Antonio E.
Mendes-Giannini, María José Soares
Zancopé-Oliveira, Rosely M.
Taylor, Maria-Lucia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rodríguez-Arellanes, Gabriela
Sousa, Carolina Nascimento de
Medeiros-Muniz, Mauro de
Ramírez, José A.
Pizzini, Cláudia V.
Abreu-Almeida, Marcos de
Oliveira, Manoel M. Evangelista de
Fusco-Almeida, Ana-Marisa
Vite-Garín, Tania
Pitangui, Nayla S.
Estrada-Bárcenas, Daniel A.
González-González, Antonio E.
Mendes-Giannini, María José Soares
Zancopé-Oliveira, Rosely M.
Taylor, Maria-Lucia
dc.subject.en.pt_BR.fl_str_mv Histoplasma capsulatum
MAT1
Mexico
Brazil
topic Histoplasma capsulatum
MAT1
Mexico
Brazil
description 2020-03-19
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2019-09-19T13:24:06Z
dc.date.available.fl_str_mv 2019-09-19T13:24:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RODRÍGUEZ-ARELLANA, Gabriela et al. Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil. Eukaryotic Cell, v. 12, n. 7, p. 1033-1038, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35677
dc.identifier.issn.pt_BR.fl_str_mv 1535-9778
dc.identifier.doi.none.fl_str_mv 10.1128/EC.00012-13
dc.identifier.eissn.none.fl_str_mv 1535-9786
identifier_str_mv RODRÍGUEZ-ARELLANA, Gabriela et al. Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil. Eukaryotic Cell, v. 12, n. 7, p. 1033-1038, 2013.
1535-9778
10.1128/EC.00012-13
1535-9786
url https://www.arca.fiocruz.br/handle/icict/35677
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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