Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/35677 |
Resumo: | 2020-03-19 |
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Rodríguez-Arellanes, GabrielaSousa, Carolina Nascimento deMedeiros-Muniz, Mauro deRamírez, José A.Pizzini, Cláudia V.Abreu-Almeida, Marcos deOliveira, Manoel M. Evangelista deFusco-Almeida, Ana-MarisaVite-Garín, TaniaPitangui, Nayla S.Estrada-Bárcenas, Daniel A.González-González, Antonio E.Mendes-Giannini, María José SoaresZancopé-Oliveira, Rosely M.Taylor, Maria-Lucia2019-09-19T13:24:06Z2019-09-19T13:24:06Z2013RODRÍGUEZ-ARELLANA, Gabriela et al. Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil. Eukaryotic Cell, v. 12, n. 7, p. 1033-1038, 2013.1535-9778https://www.arca.fiocruz.br/handle/icict/3567710.1128/EC.00012-131535-9786engAmerican Society for MicrobiologyFrequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-03-19Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. México DF, Mexico.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. México DF, Mexico.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Araraquara, SP, Brasil.Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. México DF, Mexico.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Araraquara, SP, Brasil.Instituto Politécnico Nacional. Centro de Investigación y de Estudios Avanzados. Colección Nacional de Cultivos Microbianos. México DF, Mexico.Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. México DF, Mexico.Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas. Araraquara, SP, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidad Nacional Autónoma de México. Facultad de Medicina. Departamento de Microbiología-Parasitología. México DF, Mexico.The MAT1-1 and MAT1-2 idiomorphs associated with the MAT1 locus of Histoplasma capsulatum were identified by PCR. A total of 28 fungal isolates, 6 isolates from human clinical samples and 22 isolates from environmental (infected bat and contaminated soil) samples, were studied. Among the 14 isolates from Mexico, 71.4% (95% confidence interval [95% CI], 48.3% to 94.5%) were of the MAT1-2 genotype, whereas 100% of the isolates from Brazil were of the MAT1-1 genotype. Each MAT1 idiomorphic region was sequenced and aligned, using the sequences of the G-217B (+ mating type) and G-186AR (- mating type) strains as references. BLASTn analyses of the MAT1-1 and MAT1-2 sequences studied correlated with their respective + and - mating type genotypes. Trees were generated by the maximum likelihood (ML) method to search for similarity among isolates of each MAT1 idiomorph. All MAT1-1 isolates originated from Brazilian bats formed a well-defined group; three isolates from Mexico, the G-217B strain, and a subgroup encompassing all soil-derived isolates and two clinical isolates from Brazil formed a second group; last, one isolate (EH-696P) from a migratory bat captured in Mexico formed a third group of the MAT1-1 genotype. The MAT1-2 idiomorph formed two groups, one of which included two H. capsulatum isolates from infected bats that were closely related to the G-186AR strain. The other group was formed by two human isolates and six isolates from infected bats. Concatenated ML trees, with internal transcribed spacer 1 (ITS1) -5.8S-ITS2 and MAT1-1 or MAT1-2 sequences, support the relatedness of MAT1-1 or MAT1-2 isolates. H. capsulatum mating types were associated with the geographical origin of the isolates, and all isolates from Brazil correlated with their environmental sources.Histoplasma capsulatumMAT1MexicoBrazilinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/35677/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_ Rodríguez-Arellanes_Gabriela_etal_INI_2013.pdfve_ Rodríguez-Arellanes_Gabriela_etal_INI_2013.pdfapplication/pdf443208https://www.arca.fiocruz.br/bitstream/icict/35677/2/ve_%20Rodr%c3%adguez-Arellanes_Gabriela_etal_INI_2013.pdf2008e9d7a51af037f6035bce06556548MD52TEXTve_ Rodríguez-Arellanes_Gabriela_etal_INI_2013.pdf.txtve_ Rodríguez-Arellanes_Gabriela_etal_INI_2013.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil |
title |
Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil |
spellingShingle |
Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil Rodríguez-Arellanes, Gabriela Histoplasma capsulatum MAT1 Mexico Brazil |
title_short |
Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil |
title_full |
Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil |
title_fullStr |
Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil |
title_full_unstemmed |
Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil |
title_sort |
Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil |
author |
Rodríguez-Arellanes, Gabriela |
author_facet |
Rodríguez-Arellanes, Gabriela Sousa, Carolina Nascimento de Medeiros-Muniz, Mauro de Ramírez, José A. Pizzini, Cláudia V. Abreu-Almeida, Marcos de Oliveira, Manoel M. Evangelista de Fusco-Almeida, Ana-Marisa Vite-Garín, Tania Pitangui, Nayla S. Estrada-Bárcenas, Daniel A. González-González, Antonio E. Mendes-Giannini, María José Soares Zancopé-Oliveira, Rosely M. Taylor, Maria-Lucia |
author_role |
author |
author2 |
Sousa, Carolina Nascimento de Medeiros-Muniz, Mauro de Ramírez, José A. Pizzini, Cláudia V. Abreu-Almeida, Marcos de Oliveira, Manoel M. Evangelista de Fusco-Almeida, Ana-Marisa Vite-Garín, Tania Pitangui, Nayla S. Estrada-Bárcenas, Daniel A. González-González, Antonio E. Mendes-Giannini, María José Soares Zancopé-Oliveira, Rosely M. Taylor, Maria-Lucia |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rodríguez-Arellanes, Gabriela Sousa, Carolina Nascimento de Medeiros-Muniz, Mauro de Ramírez, José A. Pizzini, Cláudia V. Abreu-Almeida, Marcos de Oliveira, Manoel M. Evangelista de Fusco-Almeida, Ana-Marisa Vite-Garín, Tania Pitangui, Nayla S. Estrada-Bárcenas, Daniel A. González-González, Antonio E. Mendes-Giannini, María José Soares Zancopé-Oliveira, Rosely M. Taylor, Maria-Lucia |
dc.subject.en.pt_BR.fl_str_mv |
Histoplasma capsulatum MAT1 Mexico Brazil |
topic |
Histoplasma capsulatum MAT1 Mexico Brazil |
description |
2020-03-19 |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2019-09-19T13:24:06Z |
dc.date.available.fl_str_mv |
2019-09-19T13:24:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RODRÍGUEZ-ARELLANA, Gabriela et al. Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil. Eukaryotic Cell, v. 12, n. 7, p. 1033-1038, 2013. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/35677 |
dc.identifier.issn.pt_BR.fl_str_mv |
1535-9778 |
dc.identifier.doi.none.fl_str_mv |
10.1128/EC.00012-13 |
dc.identifier.eissn.none.fl_str_mv |
1535-9786 |
identifier_str_mv |
RODRÍGUEZ-ARELLANA, Gabriela et al. Frequency and genetic diversity of the MAT1 locus of Histoplasma capsulatum isolates in Mexico and Brazil. Eukaryotic Cell, v. 12, n. 7, p. 1033-1038, 2013. 1535-9778 10.1128/EC.00012-13 1535-9786 |
url |
https://www.arca.fiocruz.br/handle/icict/35677 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
American Society for Microbiology |
publisher.none.fl_str_mv |
American Society for Microbiology |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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