The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin

Detalhes bibliográficos
Autor(a) principal: Salgado, Vanessa Riesz
Data de Publicação: 2016
Outros Autores: Queiroz, Artur Trancoso Lopo de, Sanabani, Sabri Saeed, Oliveira, Camila Indiani de, Carvalho Filho, Edgar Marcelino, Costa, Jackson Mauricio Lopes, Barral Netto, Manoel, Barral, Aldina Maria Prado
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14092
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Salgado, Vanessa RieszQueiroz, Artur Trancoso Lopo deSanabani, Sabri SaeedOliveira, Camila Indiani deCarvalho Filho, Edgar MarcelinoCosta, Jackson Mauricio LopesBarral Netto, ManoelBarral, Aldina Maria Prado2016-05-04T17:12:28Z2016-05-04T17:12:28Z2016SALGADO, V. R. et al. The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin. Memórias do Instituto Oswaldo Cruz, v. 111, n. 4, p. 241-251, 2016.0074-0276https://www.arca.fiocruz.br/handle/icict/14092engInstituto Oswaldo CruzThe microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / 2Instituto de Investigação em Imunologia. São Paulo, SP, BrasilUniversidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. Departamento de Patologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / 2Instituto de Investigação em Imunologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Universitário Prof Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / 2Instituto de Investigação em Imunologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / 2Instituto de Investigação em Imunologia. São Paulo, SP, BrasilLocalised cutaneous leishmaniasis (LCL) is the most common form of cutaneous leishmaniasis characterised by single or multiple painless chronic ulcers, which commonly presents with secondary bacterial infection. Previous culture- based studies have found staphylococci, streptococci, and opportunistic pathogenic bacteria in LCL lesions, but there have been no comparisons to normal skin. In addition, this approach has strong bias for determining bacterial composition. The present study tested the hypothesis that bacterial communities in LCL lesions differ from those found on healthy skin (HS). Using a high throughput amplicon sequencing approach, which allows for better populational evaluation due to greater depth coverage and the Quantitative Insights Into Microbial Ecology pipeline, we compared the microbiological signature of LCL lesions with that of contralateral HS from the same individuals. Streptococcus, Staphylococcus, Fusobacterium and other strict or facultative anaerobic bacteria composed the LCL microbiome. Aerobic and facultative anaerobic bacteria found in HS, including environmental bacteria, were significantly decreased in LCL lesions (p < 0.01). This paper presents the first comprehensive microbiome identification from LCL lesions with next generation sequence methodology and shows a marked reduction of bacterial diversity in the lesions.MicrobiomeLCLWoundHealthy skinHigh-throughputSequencinginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14092/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSalgado VR The microbiological....pdfSalgado VR The microbiological....pdfapplication/pdf2206956https://www.arca.fiocruz.br/bitstream/icict/14092/2/Salgado%20VR%20The%20microbiological....pdfe426fb702e026af9d0b5211590b5e6bdMD52TEXTSalgado VR The microbiological....pdf.txtSalgado VR The microbiological....pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin
title The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin
spellingShingle The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin
Salgado, Vanessa Riesz
Microbiome
LCL
Wound
Healthy skin
High-throughput
Sequencing
title_short The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin
title_full The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin
title_fullStr The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin
title_full_unstemmed The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin
title_sort The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin
author Salgado, Vanessa Riesz
author_facet Salgado, Vanessa Riesz
Queiroz, Artur Trancoso Lopo de
Sanabani, Sabri Saeed
Oliveira, Camila Indiani de
Carvalho Filho, Edgar Marcelino
Costa, Jackson Mauricio Lopes
Barral Netto, Manoel
Barral, Aldina Maria Prado
author_role author
author2 Queiroz, Artur Trancoso Lopo de
Sanabani, Sabri Saeed
Oliveira, Camila Indiani de
Carvalho Filho, Edgar Marcelino
Costa, Jackson Mauricio Lopes
Barral Netto, Manoel
Barral, Aldina Maria Prado
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Salgado, Vanessa Riesz
Queiroz, Artur Trancoso Lopo de
Sanabani, Sabri Saeed
Oliveira, Camila Indiani de
Carvalho Filho, Edgar Marcelino
Costa, Jackson Mauricio Lopes
Barral Netto, Manoel
Barral, Aldina Maria Prado
dc.subject.en.pt_BR.fl_str_mv Microbiome
LCL
Wound
Healthy skin
High-throughput
Sequencing
topic Microbiome
LCL
Wound
Healthy skin
High-throughput
Sequencing
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-05-04T17:12:28Z
dc.date.available.fl_str_mv 2016-05-04T17:12:28Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SALGADO, V. R. et al. The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin. Memórias do Instituto Oswaldo Cruz, v. 111, n. 4, p. 241-251, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14092
dc.identifier.issn.none.fl_str_mv 0074-0276
identifier_str_mv SALGADO, V. R. et al. The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin. Memórias do Instituto Oswaldo Cruz, v. 111, n. 4, p. 241-251, 2016.
0074-0276
url https://www.arca.fiocruz.br/handle/icict/14092
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz
publisher.none.fl_str_mv Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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