The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/14092 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
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Salgado, Vanessa RieszQueiroz, Artur Trancoso Lopo deSanabani, Sabri SaeedOliveira, Camila Indiani deCarvalho Filho, Edgar MarcelinoCosta, Jackson Mauricio LopesBarral Netto, ManoelBarral, Aldina Maria Prado2016-05-04T17:12:28Z2016-05-04T17:12:28Z2016SALGADO, V. R. et al. The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin. Memórias do Instituto Oswaldo Cruz, v. 111, n. 4, p. 241-251, 2016.0074-0276https://www.arca.fiocruz.br/handle/icict/14092engInstituto Oswaldo CruzThe microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / 2Instituto de Investigação em Imunologia. São Paulo, SP, BrasilUniversidade de São Paulo. Faculdade de Medicina. Hospital das Clínicas. Departamento de Patologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / 2Instituto de Investigação em Imunologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Universitário Prof Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / 2Instituto de Investigação em Imunologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / 2Instituto de Investigação em Imunologia. São Paulo, SP, BrasilLocalised cutaneous leishmaniasis (LCL) is the most common form of cutaneous leishmaniasis characterised by single or multiple painless chronic ulcers, which commonly presents with secondary bacterial infection. Previous culture- based studies have found staphylococci, streptococci, and opportunistic pathogenic bacteria in LCL lesions, but there have been no comparisons to normal skin. In addition, this approach has strong bias for determining bacterial composition. The present study tested the hypothesis that bacterial communities in LCL lesions differ from those found on healthy skin (HS). Using a high throughput amplicon sequencing approach, which allows for better populational evaluation due to greater depth coverage and the Quantitative Insights Into Microbial Ecology pipeline, we compared the microbiological signature of LCL lesions with that of contralateral HS from the same individuals. Streptococcus, Staphylococcus, Fusobacterium and other strict or facultative anaerobic bacteria composed the LCL microbiome. Aerobic and facultative anaerobic bacteria found in HS, including environmental bacteria, were significantly decreased in LCL lesions (p < 0.01). This paper presents the first comprehensive microbiome identification from LCL lesions with next generation sequence methodology and shows a marked reduction of bacterial diversity in the lesions.MicrobiomeLCLWoundHealthy skinHigh-throughputSequencinginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14092/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSalgado VR The microbiological....pdfSalgado VR The microbiological....pdfapplication/pdf2206956https://www.arca.fiocruz.br/bitstream/icict/14092/2/Salgado%20VR%20The%20microbiological....pdfe426fb702e026af9d0b5211590b5e6bdMD52TEXTSalgado VR The microbiological....pdf.txtSalgado VR The microbiological....pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin |
title |
The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin |
spellingShingle |
The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin Salgado, Vanessa Riesz Microbiome LCL Wound Healthy skin High-throughput Sequencing |
title_short |
The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin |
title_full |
The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin |
title_fullStr |
The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin |
title_full_unstemmed |
The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin |
title_sort |
The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin |
author |
Salgado, Vanessa Riesz |
author_facet |
Salgado, Vanessa Riesz Queiroz, Artur Trancoso Lopo de Sanabani, Sabri Saeed Oliveira, Camila Indiani de Carvalho Filho, Edgar Marcelino Costa, Jackson Mauricio Lopes Barral Netto, Manoel Barral, Aldina Maria Prado |
author_role |
author |
author2 |
Queiroz, Artur Trancoso Lopo de Sanabani, Sabri Saeed Oliveira, Camila Indiani de Carvalho Filho, Edgar Marcelino Costa, Jackson Mauricio Lopes Barral Netto, Manoel Barral, Aldina Maria Prado |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Salgado, Vanessa Riesz Queiroz, Artur Trancoso Lopo de Sanabani, Sabri Saeed Oliveira, Camila Indiani de Carvalho Filho, Edgar Marcelino Costa, Jackson Mauricio Lopes Barral Netto, Manoel Barral, Aldina Maria Prado |
dc.subject.en.pt_BR.fl_str_mv |
Microbiome LCL Wound Healthy skin High-throughput Sequencing |
topic |
Microbiome LCL Wound Healthy skin High-throughput Sequencing |
description |
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-05-04T17:12:28Z |
dc.date.available.fl_str_mv |
2016-05-04T17:12:28Z |
dc.date.issued.fl_str_mv |
2016 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SALGADO, V. R. et al. The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin. Memórias do Instituto Oswaldo Cruz, v. 111, n. 4, p. 241-251, 2016. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/14092 |
dc.identifier.issn.none.fl_str_mv |
0074-0276 |
identifier_str_mv |
SALGADO, V. R. et al. The microbiological signature of human cutaneous leishmaniasis lesions exhibits restricted bacterial diversity compared to healthy skin. Memórias do Instituto Oswaldo Cruz, v. 111, n. 4, p. 241-251, 2016. 0074-0276 |
url |
https://www.arca.fiocruz.br/handle/icict/14092 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz |
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Instituto Oswaldo Cruz |
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