A novel monoclonal antibody against canine monocytes/macrophages.
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2004 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/3267 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. |
| id |
CRUZ_6f3f48f6d1b566744403cc6e003712c6 |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/3267 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Aguiar, Paulo Henrique PalisSantos, Roberto Robson Borges dosLarangeira, Daniela FariasSantos, Marta Almeida dosMelo, Stella Maria BarrouinSilva, Tânia Maria CorreiaMengele Junior, José OrivaldodosSantos, Washington Luis ConradoPontes-de-Carvalho, Lain Carlos2011-10-20T20:45:07Z2011-10-20T20:45:07Z2004AGUIAR, Paulo Henrique Palis et al. A novel monoclonal antibody against canine monocytes/macrophages. Hybrid Hybridomics, v. 23, n. 4, p. 250-257, Aug. 2004.1536-8599https://www.arca.fiocruz.br/handle/icict/3267engA novel monoclonal antibody against canine monocytes/macrophages.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia, Escola de Medicina Veterinária. Salvador, BA, Brasil.Fundação para Desenvolvimento da Ciência, Escola Bahiana de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.The production and partial characterization of a monoclonal antibody, the IgG1 IH1, which recognizes an antigen distributed in canine monocytes/macrophages, is reported here. The distribution and apparent molecular weight of the antigen recognized by the IH1 MAb was determined in peripheral blood leukocytes, peripheral blood monocyte-derived macrophages and tissue sections of spleen, liver and skin, using Western blotting, immunocytochemistry, immunohistochemistry and flow cytometry. The IH1 MAb-recognized antigen was detected in Western blotting under non-reducing conditions spread out as a large band covering the position corresponding to the migration of molecules with molecular weights from 55 to 73 kDa. The IH1 MAb labeled blood monocytes, tissue macrophages in lymph nodes, and in the mantle zone of the spleen, and Kupffer cells in the liver. It did not react with human cells. In flow cytometric analysis, the IH1 MAb reacted with a subpopulation of monocytes. The MAb described herein may become a valuable tool for diagnosis and research on canine diseases.Anticorpos MonoclonaisWestern BlottingDiferenciação CelularCitometria de FluxoTécnica Indireta de Fluorescência para AnticorpoImunoistoquímicaMacrófagosCãesMonócitosAnimaisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALAguiar PH A novel monoclonal antibody....pdfAguiar PH A novel monoclonal antibody....pdfapplication/pdf427328https://www.arca.fiocruz.br/bitstream/icict/3267/1/Aguiar%20PH%20A%20novel%20monoclonal%20antibody....pdf9e48391ed21dca5d3847895897e92373MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3267/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTAguiar PH A novel monoclonal antibody....pdf.txtAguiar PH A novel monoclonal antibody....pdf.txtExtracted texttext/plain30222https://www.arca.fiocruz.br/bitstream/icict/3267/5/Aguiar%20PH%20A%20novel%20monoclonal%20antibody....pdf.txtc510436cffbb483717546e8df7d568eeMD55THUMBNAILAguiar PH A novel monoclonal antibody....pdf.jpgAguiar PH A novel monoclonal antibody....pdf.jpgGenerated Thumbnailimage/jpeg2243https://www.arca.fiocruz.br/bitstream/icict/3267/4/Aguiar%20PH%20A%20novel%20monoclonal%20antibody....pdf.jpg1ebfe67adc9fc27fc9ea861e438cc8faMD54icict/32672023-03-15 14:33:26.958oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:26Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
A novel monoclonal antibody against canine monocytes/macrophages. |
| title |
A novel monoclonal antibody against canine monocytes/macrophages. |
| spellingShingle |
A novel monoclonal antibody against canine monocytes/macrophages. Aguiar, Paulo Henrique Palis Anticorpos Monoclonais Western Blotting Diferenciação Celular Citometria de Fluxo Técnica Indireta de Fluorescência para Anticorpo Imunoistoquímica Macrófagos Cães Monócitos Animais |
| title_short |
A novel monoclonal antibody against canine monocytes/macrophages. |
| title_full |
A novel monoclonal antibody against canine monocytes/macrophages. |
| title_fullStr |
A novel monoclonal antibody against canine monocytes/macrophages. |
| title_full_unstemmed |
A novel monoclonal antibody against canine monocytes/macrophages. |
| title_sort |
A novel monoclonal antibody against canine monocytes/macrophages. |
| author |
Aguiar, Paulo Henrique Palis |
| author_facet |
Aguiar, Paulo Henrique Palis Santos, Roberto Robson Borges dos Larangeira, Daniela Farias Santos, Marta Almeida dos Melo, Stella Maria Barrouin Silva, Tânia Maria Correia Mengele Junior, José Orivaldo dosSantos, Washington Luis Conrado Pontes-de-Carvalho, Lain Carlos |
| author_role |
author |
| author2 |
Santos, Roberto Robson Borges dos Larangeira, Daniela Farias Santos, Marta Almeida dos Melo, Stella Maria Barrouin Silva, Tânia Maria Correia Mengele Junior, José Orivaldo dosSantos, Washington Luis Conrado Pontes-de-Carvalho, Lain Carlos |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Aguiar, Paulo Henrique Palis Santos, Roberto Robson Borges dos Larangeira, Daniela Farias Santos, Marta Almeida dos Melo, Stella Maria Barrouin Silva, Tânia Maria Correia Mengele Junior, José Orivaldo dosSantos, Washington Luis Conrado Pontes-de-Carvalho, Lain Carlos |
| dc.subject.decs.pt_BR.fl_str_mv |
Anticorpos Monoclonais Western Blotting Diferenciação Celular Citometria de Fluxo Técnica Indireta de Fluorescência para Anticorpo Imunoistoquímica Macrófagos Cães Monócitos Animais |
| topic |
Anticorpos Monoclonais Western Blotting Diferenciação Celular Citometria de Fluxo Técnica Indireta de Fluorescência para Anticorpo Imunoistoquímica Macrófagos Cães Monócitos Animais |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil. |
| publishDate |
2004 |
| dc.date.issued.fl_str_mv |
2004 |
| dc.date.accessioned.fl_str_mv |
2011-10-20T20:45:07Z |
| dc.date.available.fl_str_mv |
2011-10-20T20:45:07Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
AGUIAR, Paulo Henrique Palis et al. A novel monoclonal antibody against canine monocytes/macrophages. Hybrid Hybridomics, v. 23, n. 4, p. 250-257, Aug. 2004. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/3267 |
| dc.identifier.issn.none.fl_str_mv |
1536-8599 |
| identifier_str_mv |
AGUIAR, Paulo Henrique Palis et al. A novel monoclonal antibody against canine monocytes/macrophages. Hybrid Hybridomics, v. 23, n. 4, p. 250-257, Aug. 2004. 1536-8599 |
| url |
https://www.arca.fiocruz.br/handle/icict/3267 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/3267/1/Aguiar%20PH%20A%20novel%20monoclonal%20antibody....pdf https://www.arca.fiocruz.br/bitstream/icict/3267/2/license.txt https://www.arca.fiocruz.br/bitstream/icict/3267/5/Aguiar%20PH%20A%20novel%20monoclonal%20antibody....pdf.txt https://www.arca.fiocruz.br/bitstream/icict/3267/4/Aguiar%20PH%20A%20novel%20monoclonal%20antibody....pdf.jpg |
| bitstream.checksum.fl_str_mv |
9e48391ed21dca5d3847895897e92373 e095249ac7cacefbfe39684dfe45e706 c510436cffbb483717546e8df7d568ee 1ebfe67adc9fc27fc9ea861e438cc8fa |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1813009068859588608 |