Production of monoclonal antibodies against canine leukocytes.

Detalhes bibliográficos
Autor(a) principal: Aguiar, Paulo Henrique Palis
Data de Publicação: 2004
Outros Autores: Santos, Roberto Robson Borges dos, Lima, Carla Andrade, Gomes, Hilton Rios de Sousa, Larangeira, Daniela Farias, Santos, Patrícia Oliveira Meira, Melo, Stella Maria Barrouin, dosSantos, Washington Luis Conrado, Pontes-de-Carvalho, Lain Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/3679
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
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spelling Aguiar, Paulo Henrique PalisSantos, Roberto Robson Borges dosLima, Carla AndradeGomes, Hilton Rios de SousaLarangeira, Daniela FariasSantos, Patrícia Oliveira MeiraMelo, Stella Maria BarrouindosSantos, Washington Luis ConradoPontes-de-Carvalho, Lain Carlos2011-11-09T20:08:36Z2011-11-09T20:08:36Z2004AGUIAR, P. H. P. et al. Production of monoclonal antibodies against canine leukocytes. Hybrid Hybridomics, v. 23, n. 2, p. 127-32, apr. 2004.1536-8599https://www.arca.fiocruz.br/handle/icict/3679engProduction of monoclonal antibodies against canine leukocytes.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Department of Pathology and Clinics, Veterinary School. Salvador, BA, Brasil.Foundation for Development of Science. Bahiana School of Medicine. Salvador, BA, Brasil.Foundation for Development of Science. Bahiana School of Medicine. Salvador, BA, Brasil.Universidade Federal da Bahia. Department of Pathology and Clinics, Veterinary School. Salvador, BA, Brasil.Universidade Federal da Bahia. Department of Pathology and Clinics, Veterinary School. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.A panel of anti-canine leukocyte monoclonal antibodies (MAbs) was produced by immunizing BALB/c mice with canine peripheral blood mononuclear cells (PBMC), either resting or stimulated with concanavalin A (ConA). Three out of 28 clones—IH1, AB6, and HG6–screened by ELISA and producing antibody with the highest specificity for canine cell immunostaining, were subjected to three subsequent subcloning steps by limiting dilution, and selected for further characterization. These MAbs belonged to IgG1 (HG6 and IH1) and IgG2a (AB6) isotypes. The distribution of cell populations expressing the antigen recognized by the antibodies was identified by indirect immunoflorescence on canine PBMC and on tissue sections of lymph node, spleen, liver and skin. The possible crossreactivity with human PBMC was also examined in immunocytochemistry. One of the antibodies specifically recognized macrophages. The MAbs presented here can be foreseen as possible valuable diagnostic and research tools to study immune functions in dogs.Anticorpos MonoclonaisConcanavalinaAnimaisCãesHibridomasHumanosImunoistoquímicaLeucócitosCamundongos Endogâmicos BALB CCamundongosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALAguiar PH Production of monoclonal antibodies.pdfAguiar PH Production of monoclonal antibodies.pdfapplication/pdf359331https://www.arca.fiocruz.br/bitstream/icict/3679/1/Aguiar%20PH%20Production%20of%20monoclonal%20antibodies.pdf2e777888c8c5a6e42a1d9ee7d3c10ae7MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3679/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTAguiar PH Production of monoclonal antibodies.pdf.txtAguiar PH Production of monoclonal antibodies.pdf.txtExtracted texttext/plain24487https://www.arca.fiocruz.br/bitstream/icict/3679/5/Aguiar%20PH%20Production%20of%20monoclonal%20antibodies.pdf.txt8be62449206c0ac76a6f80a2798058edMD55THUMBNAILAguiar PH Production of monoclonal antibodies.pdf.jpgAguiar PH Production of monoclonal antibodies.pdf.jpgGenerated Thumbnailimage/jpeg2269https://www.arca.fiocruz.br/bitstream/icict/3679/4/Aguiar%20PH%20Production%20of%20monoclonal%20antibodies.pdf.jpg373969341346e9baaf14ca866ce88e8bMD54icict/36792023-03-15 14:33:02.046oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:02Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Production of monoclonal antibodies against canine leukocytes.
title Production of monoclonal antibodies against canine leukocytes.
spellingShingle Production of monoclonal antibodies against canine leukocytes.
Aguiar, Paulo Henrique Palis
Anticorpos Monoclonais
Concanavalina
Animais
Cães
Hibridomas
Humanos
Imunoistoquímica
Leucócitos
Camundongos Endogâmicos BALB C
Camundongos
title_short Production of monoclonal antibodies against canine leukocytes.
title_full Production of monoclonal antibodies against canine leukocytes.
title_fullStr Production of monoclonal antibodies against canine leukocytes.
title_full_unstemmed Production of monoclonal antibodies against canine leukocytes.
title_sort Production of monoclonal antibodies against canine leukocytes.
author Aguiar, Paulo Henrique Palis
author_facet Aguiar, Paulo Henrique Palis
Santos, Roberto Robson Borges dos
Lima, Carla Andrade
Gomes, Hilton Rios de Sousa
Larangeira, Daniela Farias
Santos, Patrícia Oliveira Meira
Melo, Stella Maria Barrouin
dosSantos, Washington Luis Conrado
Pontes-de-Carvalho, Lain Carlos
author_role author
author2 Santos, Roberto Robson Borges dos
Lima, Carla Andrade
Gomes, Hilton Rios de Sousa
Larangeira, Daniela Farias
Santos, Patrícia Oliveira Meira
Melo, Stella Maria Barrouin
dosSantos, Washington Luis Conrado
Pontes-de-Carvalho, Lain Carlos
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Aguiar, Paulo Henrique Palis
Santos, Roberto Robson Borges dos
Lima, Carla Andrade
Gomes, Hilton Rios de Sousa
Larangeira, Daniela Farias
Santos, Patrícia Oliveira Meira
Melo, Stella Maria Barrouin
dosSantos, Washington Luis Conrado
Pontes-de-Carvalho, Lain Carlos
dc.subject.decs.pt_BR.fl_str_mv Anticorpos Monoclonais
Concanavalina
Animais
Cães
Hibridomas
Humanos
Imunoistoquímica
Leucócitos
Camundongos Endogâmicos BALB C
Camundongos
topic Anticorpos Monoclonais
Concanavalina
Animais
Cães
Hibridomas
Humanos
Imunoistoquímica
Leucócitos
Camundongos Endogâmicos BALB C
Camundongos
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2011-11-09T20:08:36Z
dc.date.available.fl_str_mv 2011-11-09T20:08:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv AGUIAR, P. H. P. et al. Production of monoclonal antibodies against canine leukocytes. Hybrid Hybridomics, v. 23, n. 2, p. 127-32, apr. 2004.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/3679
dc.identifier.issn.none.fl_str_mv 1536-8599
identifier_str_mv AGUIAR, P. H. P. et al. Production of monoclonal antibodies against canine leukocytes. Hybrid Hybridomics, v. 23, n. 2, p. 127-32, apr. 2004.
1536-8599
url https://www.arca.fiocruz.br/handle/icict/3679
dc.language.iso.fl_str_mv eng
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