Morphological and biochemical characterization of macrophages activated by carrageenan and lipopolysaccharide in vivo
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2004 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/40489 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. |
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Nacife, Valéria PereiraSoeiro, Maria de Nazaré C.Gomes, Rachel NovaesD'Avila, HeloisaFaria Neto, Hugo Caire C.Meirelles, Maria de Nazareth Leal2020-03-24T18:14:54Z2020-03-24T18:14:54Z2004NACIFE, Valéria Pereira et al. Morphological and Biochemical characterization of macrophages activated by Carrageenan and Lipopolysaccaride In Vivo. Cell Structure and Function, v. 29, p. 27-34, 2004.0386-7196https://www.arca.fiocruz.br/handle/icict/4048910.1247/csf.29.271347-3700engJapan Society of Cell BiologyMacrófagosMicroscopia eletrônicaCarrageninaReceptores de LipopolissacarídeosMacrophagesCarrageenanLipopolysaccharideElectron microscopyCarrageninaReceptores de LipopolissacarídeosMorphological and biochemical characterization of macrophages activated by carrageenan and lipopolysaccharide in vivoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil.Macrophages are able to recognize, internalize and destroy a large number of pathogens, thus restricting the infection until adaptive immunity is initiated. In this work our aim was to analyze the surface charge of cells activated by carrageenan (CAR) and lipopolysaccharide (LPS) through light and electron microscopy approaches as well as the release of inflammatory mediators in vitro. The ultrastuctural analysis and the light microscopy data showed that in vivo administration of CAR represents a potent inflammatory stimulation for macrophages leading to a high degree of spreading, an increase in their size, in the number of the intracellular vacuoles and membrane projections as compared to the macrophages collected from untreated animals as well as mice submitted to LPS. Our data demonstrated that CAR stimulated-macrophages displayed a remarkable increase in nitric oxide production and PGE2 release as compared to the cells collected from non-stimulated and stimulated mice with LPS in vivo. On the other hand, non-stimulated macrophages as well as macrophages stimulated by LPS produce almost the same quantities of TNF-alpha, while in vivo stimulation by CAR leads to a 30-40% increase of cytokine release in vitro compared to the other groups. In conclusion, our morphological and biochemical data clearly showed that in vivo stimulation with CAR induces a potent inflammatory response in macrophages representing an interesting model to analyze inflammatory responses.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/40489/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMAriaNLMeireles_HugoCastro_etal_IOC_2004.pdfMAriaNLMeireles_HugoCastro_etal_IOC_2004.pdfapplication/pdf2267449https://www.arca.fiocruz.br/bitstream/icict/40489/2/MAriaNLMeireles_HugoCastro_etal_IOC_2004.pdfbdc8dd768e7ed7eb077fdb3eb44ffb8aMD52icict/404892022-06-24 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| dc.title.pt_BR.fl_str_mv |
Morphological and biochemical characterization of macrophages activated by carrageenan and lipopolysaccharide in vivo |
| title |
Morphological and biochemical characterization of macrophages activated by carrageenan and lipopolysaccharide in vivo |
| spellingShingle |
Morphological and biochemical characterization of macrophages activated by carrageenan and lipopolysaccharide in vivo Nacife, Valéria Pereira Macrófagos Microscopia eletrônica Carragenina Receptores de Lipopolissacarídeos Macrophages Carrageenan Lipopolysaccharide Electron microscopy Carragenina Receptores de Lipopolissacarídeos |
| title_short |
Morphological and biochemical characterization of macrophages activated by carrageenan and lipopolysaccharide in vivo |
| title_full |
Morphological and biochemical characterization of macrophages activated by carrageenan and lipopolysaccharide in vivo |
| title_fullStr |
Morphological and biochemical characterization of macrophages activated by carrageenan and lipopolysaccharide in vivo |
| title_full_unstemmed |
Morphological and biochemical characterization of macrophages activated by carrageenan and lipopolysaccharide in vivo |
| title_sort |
Morphological and biochemical characterization of macrophages activated by carrageenan and lipopolysaccharide in vivo |
| author |
Nacife, Valéria Pereira |
| author_facet |
Nacife, Valéria Pereira Soeiro, Maria de Nazaré C. Gomes, Rachel Novaes D'Avila, Heloisa Faria Neto, Hugo Caire C. Meirelles, Maria de Nazareth Leal |
| author_role |
author |
| author2 |
Soeiro, Maria de Nazaré C. Gomes, Rachel Novaes D'Avila, Heloisa Faria Neto, Hugo Caire C. Meirelles, Maria de Nazareth Leal |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Nacife, Valéria Pereira Soeiro, Maria de Nazaré C. Gomes, Rachel Novaes D'Avila, Heloisa Faria Neto, Hugo Caire C. Meirelles, Maria de Nazareth Leal |
| dc.subject.other.pt_BR.fl_str_mv |
Macrófagos Microscopia eletrônica Carragenina Receptores de Lipopolissacarídeos |
| topic |
Macrófagos Microscopia eletrônica Carragenina Receptores de Lipopolissacarídeos Macrophages Carrageenan Lipopolysaccharide Electron microscopy Carragenina Receptores de Lipopolissacarídeos |
| dc.subject.en.pt_BR.fl_str_mv |
Macrophages Carrageenan Lipopolysaccharide Electron microscopy |
| dc.subject.decs.pt_BR.fl_str_mv |
Carragenina Receptores de Lipopolissacarídeos |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ultra-estrutura e Biologia Celular. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2004 |
| dc.date.issued.fl_str_mv |
2004 |
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2020-03-24T18:14:54Z |
| dc.date.available.fl_str_mv |
2020-03-24T18:14:54Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
NACIFE, Valéria Pereira et al. Morphological and Biochemical characterization of macrophages activated by Carrageenan and Lipopolysaccaride In Vivo. Cell Structure and Function, v. 29, p. 27-34, 2004. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/40489 |
| dc.identifier.issn.pt_BR.fl_str_mv |
0386-7196 |
| dc.identifier.doi.none.fl_str_mv |
10.1247/csf.29.27 |
| dc.identifier.eissn.none.fl_str_mv |
1347-3700 |
| identifier_str_mv |
NACIFE, Valéria Pereira et al. Morphological and Biochemical characterization of macrophages activated by Carrageenan and Lipopolysaccaride In Vivo. Cell Structure and Function, v. 29, p. 27-34, 2004. 0386-7196 10.1247/csf.29.27 1347-3700 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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Japan Society of Cell Biology |
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Japan Society of Cell Biology |
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