Curine inhibits macrophage activation and neutrophil recruitment in a mouse model of lipopolysaccharide-induced inflammation

Detalhes bibliográficos
Autor(a) principal: Ribeiro Filho, Jaime
Data de Publicação: 2019
Outros Autores: Leite, Fagner Carvalho, Calheiros, Andrea Surrage, Carneiro, Alan de Brito, Azeredo, Juliana Alves, Assis, Edson Fernandes de, Dias, Celidarque da Silva, Piuvezam, Márcia Regina, Bozza, Patrícia T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/39336
Resumo: PRONEX/MCT
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spelling Ribeiro Filho, JaimeLeite, Fagner CarvalhoCalheiros, Andrea SurrageCarneiro, Alan de BritoAzeredo, Juliana AlvesAssis, Edson Fernandes deDias, Celidarque da SilvaPiuvezam, Márcia ReginaBozza, Patrícia T.2020-01-21T12:21:53Z2020-01-21T12:21:53Z2019RIBEIRO FILHO, Jaime et al. Curine inhibits macrophage activation and neutrophil recruitment in a mouse model of lipopolysaccharide-induced inflammation. Toxins, v. 11, n. 12, p. 1-12, 3 Dec. 2019.2072-6651https://www.arca.fiocruz.br/handle/icict/3933610.3390/toxins11120705PRONEX/MCTCNPqFAPERJINCT-CâncerFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Laboratório de Investigação em Genética e Hematologia Translacional. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Laboratório de Pesquisa em Anemias. Departamento de Análises Clínicas e Toxicologicas. Salvador, BA, Brasil.Universidade Federal da Paraíba. Laboratório de Imunofarmacologia. Departamento de Fisiologia e Patologia. João Pessoa, PB, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Universidade Federal da Paraíba. Laboratório de Fitoquímica. Departamento de Ciências Farmacêuticas. João Pessoa, PB, Brasil.Universidade Federal da Paraíba. Laboratório de Imunofarmacologia. Departamento de Fisiologia e Patologia. João Pessoa, PB, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Curine is a bisbenzylisoquinoline alkaloid (BBA) with anti-allergic, analgesic, and anti-inflammatory properties. Previous studies have demonstrated that this alkaloid is orally active at non-toxic doses. However, the mechanisms underlying its anti-inflammatory effects remain to be elucidated. This work aimed to investigate the effects of curine on macrophage activation and neutrophil recruitment. Using a murine model of lipopolysaccharide (LPS)-induced pleurisy, we demonstrated that curine significantly inhibited the recruitment of neutrophils in association with the inhibition of cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, IL-6, monocyte chemotactic protein (CCL2/MCP-1) as well as leukotriene B4 in the pleural lavage of mice. Curine treatment reduced cytokine levels and the expression of iNOS in in vitro cultures of macrophages stimulated with LPS. Treatment with a calcium channel blocker resulted in comparable inhibition of TNF-α and IL-1β production, as well as iNOS expression by macrophages, suggesting that the anti-inflammatory effects of curine may be related to the inhibition of calcium-dependent mechanisms involved in macrophage activation. In conclusion, curine presented anti-inflammatory effects that are associated with inhibition of macrophage activation and neutrophil recruitment by inhibiting the production of inflammatory cytokines, LTB4 and nitric oxide (NO), and possibly by negatively modulating Ca2+ influx.engMDPICurineAlcalóideMacrófagoNeutrófiloLipopolissacarídeoCurineAlkaloidMacrophageNeutrophilLipopolysaccharideCurine inhibits macrophage activation and neutrophil recruitment in a mouse model of lipopolysaccharide-induced inflammationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/39336/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRibeiro-Filho j Curine Inhibits Tosins.pdfRibeiro-Filho j Curine Inhibits Tosins.pdfapplication/pdf4153596https://www.arca.fiocruz.br/bitstream/icict/39336/2/Ribeiro-Filho%20j%20Curine%20Inhibits%20Tosins.pdfcc6b46647db9fe4e1ee57c25f4dcf6a8MD52TEXTRibeiro-Filho j Curine Inhibits Tosins.pdf.txtRibeiro-Filho j Curine Inhibits Tosins.pdf.txtExtracted texttext/plain52014https://www.arca.fiocruz.br/bitstream/icict/39336/3/Ribeiro-Filho%20j%20Curine%20Inhibits%20Tosins.pdf.txt15b96b76f200af408338e758ff184d4eMD53icict/393362023-03-15 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dc.title.en.fl_str_mv Curine inhibits macrophage activation and neutrophil recruitment in a mouse model of lipopolysaccharide-induced inflammation
title Curine inhibits macrophage activation and neutrophil recruitment in a mouse model of lipopolysaccharide-induced inflammation
spellingShingle Curine inhibits macrophage activation and neutrophil recruitment in a mouse model of lipopolysaccharide-induced inflammation
Ribeiro Filho, Jaime
Curine
Alcalóide
Macrófago
Neutrófilo
Lipopolissacarídeo
Curine
Alkaloid
Macrophage
Neutrophil
Lipopolysaccharide
title_short Curine inhibits macrophage activation and neutrophil recruitment in a mouse model of lipopolysaccharide-induced inflammation
title_full Curine inhibits macrophage activation and neutrophil recruitment in a mouse model of lipopolysaccharide-induced inflammation
title_fullStr Curine inhibits macrophage activation and neutrophil recruitment in a mouse model of lipopolysaccharide-induced inflammation
title_full_unstemmed Curine inhibits macrophage activation and neutrophil recruitment in a mouse model of lipopolysaccharide-induced inflammation
title_sort Curine inhibits macrophage activation and neutrophil recruitment in a mouse model of lipopolysaccharide-induced inflammation
author Ribeiro Filho, Jaime
author_facet Ribeiro Filho, Jaime
Leite, Fagner Carvalho
Calheiros, Andrea Surrage
Carneiro, Alan de Brito
Azeredo, Juliana Alves
Assis, Edson Fernandes de
Dias, Celidarque da Silva
Piuvezam, Márcia Regina
Bozza, Patrícia T.
author_role author
author2 Leite, Fagner Carvalho
Calheiros, Andrea Surrage
Carneiro, Alan de Brito
Azeredo, Juliana Alves
Assis, Edson Fernandes de
Dias, Celidarque da Silva
Piuvezam, Márcia Regina
Bozza, Patrícia T.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ribeiro Filho, Jaime
Leite, Fagner Carvalho
Calheiros, Andrea Surrage
Carneiro, Alan de Brito
Azeredo, Juliana Alves
Assis, Edson Fernandes de
Dias, Celidarque da Silva
Piuvezam, Márcia Regina
Bozza, Patrícia T.
dc.subject.other.pt_BR.fl_str_mv Curine
Alcalóide
Macrófago
Neutrófilo
Lipopolissacarídeo
topic Curine
Alcalóide
Macrófago
Neutrófilo
Lipopolissacarídeo
Curine
Alkaloid
Macrophage
Neutrophil
Lipopolysaccharide
dc.subject.en.pt_BR.fl_str_mv Curine
Alkaloid
Macrophage
Neutrophil
Lipopolysaccharide
description PRONEX/MCT
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-01-21T12:21:53Z
dc.date.available.fl_str_mv 2020-01-21T12:21:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RIBEIRO FILHO, Jaime et al. Curine inhibits macrophage activation and neutrophil recruitment in a mouse model of lipopolysaccharide-induced inflammation. Toxins, v. 11, n. 12, p. 1-12, 3 Dec. 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/39336
dc.identifier.issn.pt_BR.fl_str_mv 2072-6651
dc.identifier.doi.pt_BR.fl_str_mv 10.3390/toxins11120705
identifier_str_mv RIBEIRO FILHO, Jaime et al. Curine inhibits macrophage activation and neutrophil recruitment in a mouse model of lipopolysaccharide-induced inflammation. Toxins, v. 11, n. 12, p. 1-12, 3 Dec. 2019.
2072-6651
10.3390/toxins11120705
url https://www.arca.fiocruz.br/handle/icict/39336
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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