Toxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: a promising ocular toxoplasmosis model

Detalhes bibliográficos
Autor(a) principal: Nasaré, Alex M.
Data de Publicação: 2015
Outros Autores: Tedesco, Roberto C., Cristovam, Priscila C., Cenedese, Marcos A., Galisteo Jr., Andrés J., Andrade Jr., Heitor F., Gomes, José Álvaro P., Guimarães, Érik V., Barbosa, Helene S., Alonso, Luis G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14397
Resumo: Universidade Federal de São Paulo. Departamento de Genética e Morfologia. Botucatu, SP, Brasil.
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spelling Nasaré, Alex M.Tedesco, Roberto C.Cristovam, Priscila C.Cenedese, Marcos A.Galisteo Jr., Andrés J.Andrade Jr., Heitor F.Gomes, José Álvaro P.Guimarães, Érik V.Barbosa, Helene S.Alonso, Luis G.2016-05-19T16:11:15Z2016-05-19T16:11:15Z2015NASARÉ, Alex M. et al. Toxoplasma Gondii Infection of Chicken Embryos Causes Retinal Changes and Modulates HSP90B1 Gene Expression: A Promising Ocular Toxoplasmosis Model. Eur J Microbiol Immunol (Bp), v.5, n.4, p.316-20, Nov. 2015.2062-8633https://www.arca.fiocruz.br/handle/icict/1439710.1556/1886.2015.00024engElsevierDesenvolvimento embrionárioModelos experimentaisToxoplasmose ocularInfecções oculares parasitáriasRetinaEmbryo developmentExperimental modelsOcular toxoplasmosisParasitic ocular infectionsRegulation of gene expression in developmenRetinaToxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: a promising ocular toxoplasmosis modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de São Paulo. Departamento de Genética e Morfologia. Botucatu, SP, Brasil.Universidade Federal de São Paulo. Departamento de Genética e Morfologia. Botucatu, SP, Brasil.Instituto de Medicina Tropical de São Paulo. Departamento de Oftalmologia. Botucatu, SP, Brasil.Universidade Federal de São Paulo. Disciplina de Nefrologia. Botucatu, SP, Brasil.Universidade Federal de São Paulo. Departamento de Protozoologia. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Departamento de Protozoologia. São Paulo, SP, Brasil.Instituto de Medicina Tropical de São Paulo. Departamento de Oftalmologia. Botucatu, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Universidade Federal de São Paulo. Departamento de Genética e Morfologia. Botucatu, SP, Brasil.HSP90B1 is a gene that codifies heat shock protein 108 (HSP108) that belongs to a group of proteins induced under stress situation, and it has close relation with the nervous system, especially in the retina. Toxoplasma gondii causes ocular toxoplasmosis that has been associated with a late manifestation of the congenital toxoplasmosis although experimental models show that morphological alterations are already present during embryological development. Here, we used 18 eyes of Gallus domesticus embryos in 7th and 20th embryonic days to establish a model of congenital ocular toxoplasmosis, experimentally infected in its fifth day correlating with HSP90B1 gene expression. Embryos' eyes were histologically evaluated, and gene expression was performed by real-time polymerase chain reaction (PCR). Our data showed parasite present in the choroid, unusual migration of retinal pigment epithelium, and chorioretinal scars, and a tendency to a lower expression of the HSP90B1 gene upon experimental infection. This is a promising model to better understand T. gondii etiopathogeny.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14397/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALerik_guimaraes_etal_IOC_2015.pdferik_guimaraes_etal_IOC_2015.pdfapplication/pdf441857https://www.arca.fiocruz.br/bitstream/icict/14397/2/erik_guimaraes_etal_IOC_2015.pdf1b9f72499c7ee0799049af5364ad0a6eMD52TEXTerik_guimaraes_etal_IOC_2015.pdf.txterik_guimaraes_etal_IOC_2015.pdf.txtExtracted texttext/plain18882https://www.arca.fiocruz.br/bitstream/icict/14397/3/erik_guimaraes_etal_IOC_2015.pdf.txt2eb04b018b2d1f98c1e7b2c733da94a5MD53icict/143972018-04-02 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dc.title.pt_BR.fl_str_mv Toxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: a promising ocular toxoplasmosis model
title Toxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: a promising ocular toxoplasmosis model
spellingShingle Toxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: a promising ocular toxoplasmosis model
Nasaré, Alex M.
Desenvolvimento embrionário
Modelos experimentais
Toxoplasmose ocular
Infecções oculares parasitárias
Retina
Embryo development
Experimental models
Ocular toxoplasmosis
Parasitic ocular infections
Regulation of gene expression in developmen
Retina
title_short Toxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: a promising ocular toxoplasmosis model
title_full Toxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: a promising ocular toxoplasmosis model
title_fullStr Toxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: a promising ocular toxoplasmosis model
title_full_unstemmed Toxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: a promising ocular toxoplasmosis model
title_sort Toxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: a promising ocular toxoplasmosis model
author Nasaré, Alex M.
author_facet Nasaré, Alex M.
Tedesco, Roberto C.
Cristovam, Priscila C.
Cenedese, Marcos A.
Galisteo Jr., Andrés J.
Andrade Jr., Heitor F.
Gomes, José Álvaro P.
Guimarães, Érik V.
Barbosa, Helene S.
Alonso, Luis G.
author_role author
author2 Tedesco, Roberto C.
Cristovam, Priscila C.
Cenedese, Marcos A.
Galisteo Jr., Andrés J.
Andrade Jr., Heitor F.
Gomes, José Álvaro P.
Guimarães, Érik V.
Barbosa, Helene S.
Alonso, Luis G.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nasaré, Alex M.
Tedesco, Roberto C.
Cristovam, Priscila C.
Cenedese, Marcos A.
Galisteo Jr., Andrés J.
Andrade Jr., Heitor F.
Gomes, José Álvaro P.
Guimarães, Érik V.
Barbosa, Helene S.
Alonso, Luis G.
dc.subject.other.pt_BR.fl_str_mv Desenvolvimento embrionário
Modelos experimentais
Toxoplasmose ocular
Infecções oculares parasitárias
Retina
topic Desenvolvimento embrionário
Modelos experimentais
Toxoplasmose ocular
Infecções oculares parasitárias
Retina
Embryo development
Experimental models
Ocular toxoplasmosis
Parasitic ocular infections
Regulation of gene expression in developmen
Retina
dc.subject.en.pt_BR.fl_str_mv Embryo development
Experimental models
Ocular toxoplasmosis
Parasitic ocular infections
Regulation of gene expression in developmen
Retina
description Universidade Federal de São Paulo. Departamento de Genética e Morfologia. Botucatu, SP, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-05-19T16:11:15Z
dc.date.available.fl_str_mv 2016-05-19T16:11:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NASARÉ, Alex M. et al. Toxoplasma Gondii Infection of Chicken Embryos Causes Retinal Changes and Modulates HSP90B1 Gene Expression: A Promising Ocular Toxoplasmosis Model. Eur J Microbiol Immunol (Bp), v.5, n.4, p.316-20, Nov. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14397
dc.identifier.issn.none.fl_str_mv 2062-8633
dc.identifier.doi.none.fl_str_mv 10.1556/1886.2015.00024
identifier_str_mv NASARÉ, Alex M. et al. Toxoplasma Gondii Infection of Chicken Embryos Causes Retinal Changes and Modulates HSP90B1 Gene Expression: A Promising Ocular Toxoplasmosis Model. Eur J Microbiol Immunol (Bp), v.5, n.4, p.316-20, Nov. 2015.
2062-8633
10.1556/1886.2015.00024
url https://www.arca.fiocruz.br/handle/icict/14397
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/14397/2/erik_guimaraes_etal_IOC_2015.pdf
https://www.arca.fiocruz.br/bitstream/icict/14397/3/erik_guimaraes_etal_IOC_2015.pdf.txt
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