High correlation between Chagas’ disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 1994 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/14675 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. |
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Wincker, PatrickBosseno, Marie-FranceBritto, ConstançaYaksic, NinaCardoso, Maria AngélicaMorel, Carlos MedicisBrenière, Simone Frédérique2016-06-29T15:43:03Z2016-07-07T12:13:56Z2016-06-29T15:43:03Z2016-07-07T12:13:56Z1994WINCKER, Patrick et al. High correlation between chagas'disease serology and pcr-based detection of trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area. FEMS Microbiology Letters, v. 124, p. 419-424, 1994.0378-1097https://www.arca.fiocruz.br/handle/icict/1467510.1111/j.1574-6968.1994.tb07318.x1574-6968engWileyHigh correlation between Chagas’ disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic areainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.UMR CNRS/ ORSTOM, Génétique Moléculaire des Parasites et des Vecteurs. CP 9214, La Paz, Bolivia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Universidad Mayor de San Andres. Instituto Boliviano de Biologia de Altura. La Paz, Bolivia.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.UMR CNRS/ ORSTOM, Génétique Moléculaire des Parasites et des Vecteurs. CP 9214, La Paz, Bolivia.The detection of Tr)tpunosomu crirzi kinetoplast DNA by polymerase chain reaction (PCR) amplification is a potentially powerful tool for the parasitological diagnosis of Chagas’ disease. We have applied this technique in a field situation in Bolivia, where 45 children from a primary school were subjected to serological testing, buffy coat analysis and PCR diagnosis. 26 of the 28 serology-positive individuals were also positive by PCR. In addition, two serology-negative children gave a positive result by PCR, including one who was positive in the buffy coat test. These results suggest that PCR detection of T. cruzi DNA in blood can be a very useful complement to serology in Chagas’ disease diagnosis in Bolivia.Trypanosoma cruziChagas`DiseaseKinetoplast DNAPCR DiagonosisBoliviaTrypanosoma cruziDoença de ChagasDNA de CinetoplastoReação em Cadeia da PolimeraseDiagnósticoBolíviainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTcarlos20_morel_etal_IOC_1994.pdf.txtcarlos20_morel_etal_IOC_1994.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
High correlation between Chagas’ disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area |
| title |
High correlation between Chagas’ disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area |
| spellingShingle |
High correlation between Chagas’ disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area Wincker, Patrick Trypanosoma cruzi Chagas`Disease Kinetoplast DNA PCR Diagonosis Bolivia Trypanosoma cruzi Doença de Chagas DNA de Cinetoplasto Reação em Cadeia da Polimerase Diagnóstico Bolívia |
| title_short |
High correlation between Chagas’ disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area |
| title_full |
High correlation between Chagas’ disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area |
| title_fullStr |
High correlation between Chagas’ disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area |
| title_full_unstemmed |
High correlation between Chagas’ disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area |
| title_sort |
High correlation between Chagas’ disease serology and PCR-based detection of Trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area |
| author |
Wincker, Patrick |
| author_facet |
Wincker, Patrick Bosseno, Marie-France Britto, Constança Yaksic, Nina Cardoso, Maria Angélica Morel, Carlos Medicis Brenière, Simone Frédérique |
| author_role |
author |
| author2 |
Bosseno, Marie-France Britto, Constança Yaksic, Nina Cardoso, Maria Angélica Morel, Carlos Medicis Brenière, Simone Frédérique |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Wincker, Patrick Bosseno, Marie-France Britto, Constança Yaksic, Nina Cardoso, Maria Angélica Morel, Carlos Medicis Brenière, Simone Frédérique |
| dc.subject.en.pt_BR.fl_str_mv |
Trypanosoma cruzi Chagas`Disease Kinetoplast DNA PCR Diagonosis Bolivia |
| topic |
Trypanosoma cruzi Chagas`Disease Kinetoplast DNA PCR Diagonosis Bolivia Trypanosoma cruzi Doença de Chagas DNA de Cinetoplasto Reação em Cadeia da Polimerase Diagnóstico Bolívia |
| dc.subject.decs.pt_BR.fl_str_mv |
Trypanosoma cruzi Doença de Chagas DNA de Cinetoplasto Reação em Cadeia da Polimerase Diagnóstico Bolívia |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. |
| publishDate |
1994 |
| dc.date.issued.fl_str_mv |
1994 |
| dc.date.accessioned.fl_str_mv |
2016-06-29T15:43:03Z 2016-07-07T12:13:56Z |
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2016-06-29T15:43:03Z 2016-07-07T12:13:56Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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| dc.identifier.citation.fl_str_mv |
WINCKER, Patrick et al. High correlation between chagas'disease serology and pcr-based detection of trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area. FEMS Microbiology Letters, v. 124, p. 419-424, 1994. |
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https://www.arca.fiocruz.br/handle/icict/14675 |
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0378-1097 |
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10.1111/j.1574-6968.1994.tb07318.x |
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1574-6968 |
| identifier_str_mv |
WINCKER, Patrick et al. High correlation between chagas'disease serology and pcr-based detection of trypanosoma cruzi kinetoplast DNA in Bolivian children living in an endemic area. FEMS Microbiology Letters, v. 124, p. 419-424, 1994. 0378-1097 10.1111/j.1574-6968.1994.tb07318.x 1574-6968 |
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Wiley |
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