Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction

Detalhes bibliográficos
Autor(a) principal: Britto, Constança
Data de Publicação: 2001
Outros Autores: Silveira, Celeste, Cardoso, Maria Angelica, Marques, Patricia, Luquetti, Alejandro, Macêdo, Vanize, Fernandes, Octávio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44152
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Britto, ConstançaSilveira, CelesteCardoso, Maria AngelicaMarques, PatriciaLuquetti, AlejandroMacêdo, VanizeFernandes, Octávio2020-10-23T19:43:20Z2020-10-23T19:43:20Z2001BRITTO, Constança et al. Parasite Persistence in Treated Chagasic Patients Revealed by Xenodiagnosis and Polymerase Chain Reaction. Memórias do instituto Oswaldo Cruz, Rio de Janeiro, v. 96, 4p, 2001.0074-0276https://www.arca.fiocruz.br/handle/icict/441521678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Trypanosoma cruziDoença de ChagasReação em Cadeia da PolimeraseTratamentoDNA de cinetoplastoTrypanosoma cruzikinetoplast DNAChagas diseasePCRTreatmentParasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reactioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade de Brasília. Departamento de Medicina Tropical. Brasília, DF, Brasil.Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, Go, Brasil.Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, Go, Brasil.Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, Go, Brasil.Universidade de Brasília. Departamento de Medicina Tropical. Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Rio de Janeiro, RJ, Brasil.Polymerase chain reaction (PCR) was compared with xenodiagnosis performed 20 years after trypanocidal chemotherapy to investigate parasite clearance. Eighty-five seropositive individuals for Chagas disease presenting a positive xenodiagnosis were treated with specific drugs; 37 in the acute phase and 48 in the chronic phase. Fifteen chronic assymptomatic patients received a placebo. Treatment in the acute phase led to PCR negative results in 73% of the cases, while xenodiagnosis was negative in 86%. In the chronic phase, PCR was negative in 65% of the patients and 83% led to xenodiagnosis negative results. Regarding the untreated group (placebo), 73% gave negative results by xenodiagnosis, of which 36% were positive by PCR. Individuals that were considered seronegative (n=10), presented unequivocally negative results in the PCR demonstrating the elimination of parasite DNA. Seventeen individuals had their antibodies titers decreased to such a level that the final results were considered as doubtful and 16 of them presented negative PCR. The molecular method represents a clear advantage over conventional techniques to demonstrate persistent infections in Chagas disease patients that underwent chemotherapy.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44152/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALConstançaBrito_CelesteSilveira_etal_IOC_2001.pdfConstançaBrito_CelesteSilveira_etal_IOC_2001.pdfapplication/pdf41134https://www.arca.fiocruz.br/bitstream/icict/44152/2/Constan%c3%a7aBrito_CelesteSilveira_etal_IOC_2001.pdf6c44e7d593709af09f2af0d61546a012MD52TEXTConstançaBrito_CelesteSilveira_etal_IOC_2001.pdf.txtConstançaBrito_CelesteSilveira_etal_IOC_2001.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
title Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
spellingShingle Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
Britto, Constança
Trypanosoma cruzi
Doença de Chagas
Reação em Cadeia da Polimerase
Tratamento
DNA de cinetoplasto
Trypanosoma cruzi
kinetoplast DNA
Chagas disease
PCR
Treatment
title_short Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
title_full Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
title_fullStr Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
title_full_unstemmed Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
title_sort Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
author Britto, Constança
author_facet Britto, Constança
Silveira, Celeste
Cardoso, Maria Angelica
Marques, Patricia
Luquetti, Alejandro
Macêdo, Vanize
Fernandes, Octávio
author_role author
author2 Silveira, Celeste
Cardoso, Maria Angelica
Marques, Patricia
Luquetti, Alejandro
Macêdo, Vanize
Fernandes, Octávio
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Britto, Constança
Silveira, Celeste
Cardoso, Maria Angelica
Marques, Patricia
Luquetti, Alejandro
Macêdo, Vanize
Fernandes, Octávio
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Doença de Chagas
Reação em Cadeia da Polimerase
Tratamento
DNA de cinetoplasto
topic Trypanosoma cruzi
Doença de Chagas
Reação em Cadeia da Polimerase
Tratamento
DNA de cinetoplasto
Trypanosoma cruzi
kinetoplast DNA
Chagas disease
PCR
Treatment
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
kinetoplast DNA
Chagas disease
PCR
Treatment
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2020-10-23T19:43:20Z
dc.date.available.fl_str_mv 2020-10-23T19:43:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BRITTO, Constança et al. Parasite Persistence in Treated Chagasic Patients Revealed by Xenodiagnosis and Polymerase Chain Reaction. Memórias do instituto Oswaldo Cruz, Rio de Janeiro, v. 96, 4p, 2001.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44152
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv BRITTO, Constança et al. Parasite Persistence in Treated Chagasic Patients Revealed by Xenodiagnosis and Polymerase Chain Reaction. Memórias do instituto Oswaldo Cruz, Rio de Janeiro, v. 96, 4p, 2001.
0074-0276
1678-8060
url https://www.arca.fiocruz.br/handle/icict/44152
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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