Transcriptome analysis of gossypium hirsutum flower buds infested by cotton boll weevil (anthonomus grandis) larvae

Detalhes bibliográficos
Autor(a) principal: Artico, Sinara
Data de Publicação: 2014
Outros Autores: Alves, Marcelo Ribeiro, Oliveira Neto, Osmundo Brilhante, Macedo, Leonardo Lima Pepino de, Silveira, Sylvia, Sa, Maria Fátima Grossi de, Martinelli, Adriana Pinheiro, Ferreira, Marcio Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11051
Resumo: We thank Prof. S.M. Tsai, Cell and Molecular Biology Laboratory, CENA/USP, for maintaining the multiuser LMD facility (FAPESP 2009/53998-3). APM acknowledges CNPq (Grant 310.612/2011-0). The authors gratefully thank Marco A. Takita and Carolina M. Rodrigues for use of the computational structure in the Laboratory of Biotechnology, Center for Citrus Sylvio Moreira, Cordeirópolis-Sao Paulo, Brazil. We thank Julia Lambret-Frotté for help us with the phylogenetic analysis. This work was part of S.A’s Ph. D. research in Genetics at the Department of Genetics of the Universidade Federal do Rio de Janeiro (UFRJ) and was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; M. Alves-Ferreira: # 306025/2010-8) and Fundação de Amparo à Pesquisa do Rio de Janeiro (FAPERJ).
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spelling Artico, SinaraAlves, Marcelo RibeiroOliveira Neto, Osmundo BrilhanteMacedo, Leonardo Lima Pepino deSilveira, SylviaSa, Maria Fátima Grossi deMartinelli, Adriana PinheiroFerreira, Marcio Alves2015-07-01T18:37:30Z2015-07-01T18:37:30Z2014ARTICO, Sinara et al. Transcriptome analysis of Gossypium hirsutum flower buds infested by cotton boll weevil (Anthonomus grandis) larvae. BMC Genomics, v.15, n.854, p.1-24, 2014.1471-2164https://www.arca.fiocruz.br/handle/icict/1105110.1186/1471-2164-15-8541471-2164We thank Prof. S.M. Tsai, Cell and Molecular Biology Laboratory, CENA/USP, for maintaining the multiuser LMD facility (FAPESP 2009/53998-3). APM acknowledges CNPq (Grant 310.612/2011-0). The authors gratefully thank Marco A. Takita and Carolina M. Rodrigues for use of the computational structure in the Laboratory of Biotechnology, Center for Citrus Sylvio Moreira, Cordeirópolis-Sao Paulo, Brazil. We thank Julia Lambret-Frotté for help us with the phylogenetic analysis. This work was part of S.A’s Ph. D. research in Genetics at the Department of Genetics of the Universidade Federal do Rio de Janeiro (UFRJ) and was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; M. Alves-Ferreira: # 306025/2010-8) and Fundação de Amparo à Pesquisa do Rio de Janeiro (FAPERJ).Universidade Federal do Rio de Janeiro. Departamento de Genetica. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST e AIDS. Rio de Janeiro, RJ, BrasilEmbrapa Recursos Genéticos e Biotecnologia. Brasília, DF, Brasil / Escola de Medicina Faciplac. Departamento de Bioquímica e Biologia Molecular. Brasília, DF, BrasilEmbrapa Recursos Genéticos e Biotecnologia. Brasília, DF, BrasilUniversidade de São Paulo. Piracicaba, SP, BrasilEmbrapa Recursos Genéticos e Biotecnologia. Brasília, DF, Brasil / Universidade Católica de Brasília. Brasília, DF, BrasilUniversidade de São Paulo. Piracicaba, SP, BrasilUniversidade Federal do Rio de Janeiro. Departamento de Genetica. Rio de Janeiro, RJ, BrasilBackground: Cotton is a major fibre crop grown worldwide that suffers extensive damage from chewing insects, including the cotton boll weevil larvae (Anthonomus grandis). Transcriptome analysis was performed to understand the molecular interactions between Gossypium hirsutum L. and cotton boll weevil larvae. The Illumina HiSeq 2000 platform was used to sequence the transcriptome of cotton flower buds infested with boll weevil larvae. Results: The analysis generated a total of 327,489,418 sequence reads that were aligned to the G. hirsutum reference transcriptome. The total number of expressed genes was over 21,697 per sample with an average length of 1,063 bp. The DEGseq analysis identified 443 differentially expressed genes (DEG) in cotton flower buds infected with boll weevil larvae. Among them, 402 (90.7%) were up-regulated, 41 (9.3%) were down-regulated and 432 (97.5%) were identified as orthologues of A. thaliana genes using Blastx. Mapman analysis of DEG indicated that many genes were involved in the biotic stress response spanning a range of functions, from a gene encoding a receptor-like kinase to genes involved in triggering defensive responses such as MAPK, transcription factors (WRKY and ERF) and signalling by ethylene (ET) and jasmonic acid (JA) hormones. Furthermore, the spatial expression pattern of 32 of the genes responsive to boll weevil larvae feeding was determined by “in situ” qPCR analysis from RNA isolated from two flower structures, the stamen and the carpel, by laser microdissection (LMD). Conclusion: A large number of cotton transcripts were significantly altered upon infestation by larvae. Among the changes in gene expression, we highlighted the transcription of receptors/sensors that recognise chitin or insect oral secretions; the altered regulation of transcripts encoding enzymes related to kinase cascades, transcription factors, Ca2+ influxes, and reactive oxygen species; and the modulation of transcripts encoding enzymes from phytohormone signalling pathways. These data will aid in the selection of target genes to genetically engineer cotton to control the cotton boll weevil.engBMC GenomicsLarvaSequenciamento TranscriptomaEstresse BióticoTranscriptomeLaser Capture MicrodissectionGossypiumRNALarvaeTranscriptome SequencingBiotic StressTranscriptomaMicrodissecção e Captura a LaserGossypiumRNATranscriptome analysis of gossypium hirsutum flower buds infested by cotton boll weevil (anthonomus grandis) larvaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/11051/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALTranscriptome analysis of Gossypium hirsutum.pdfTranscriptome analysis of Gossypium hirsutum.pdfapplication/pdf2247313https://www.arca.fiocruz.br/bitstream/icict/11051/2/Transcriptome%20analysis%20of%20Gossypium%20hirsutum.pdfab7c8ebe86d265a46f75dc18a173d623MD52TEXTTranscriptome analysis of Gossypium hirsutum.pdf.txtTranscriptome analysis of Gossypium hirsutum.pdf.txtExtracted texttext/plain112996https://www.arca.fiocruz.br/bitstream/icict/11051/3/Transcriptome%20analysis%20of%20Gossypium%20hirsutum.pdf.txt5a6c0d01819b589c3928ffb3f773d7b5MD53icict/110512018-04-04 08:26:28.3oai:www.arca.fiocruz.br:icict/11051Q0VTU8ODTyBOw4NPLUVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcyBhdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKQ0VERSBlIFRSQU5TRkVSRSwgdG90YWwgZSBncmF0dWl0YW1lbnRlLCDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVosIGVtIGNhcsOhdGVyIHBlcm1hbmVudGUsIGlycmV2b2fDoXZlbCBlIE7Dg08gRVhDTFVTSVZPLCAKdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08gQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcyAKZGUgdm96IGUgaW1hZ2VtIHZpbmN1bGFkb3Mgw6AgT0JSQSwgZHVyYW50ZSB0b2RvIG8gcHJhem8gZGUgZHVyYcOnw6NvIGRvcyBkaXJlaXRvcyBhdXRvcmFpcywgZW0gcXVhbHF1ZXIgaWRpb21hIGUgZW0gdG9kb3Mgb3MgcGHDrXNlczsKICAgICAgICAKQUNFSVRBIHF1ZSBhIGNlc3PDo28gdG90YWwgbsOjbyBleGNsdXNpdmEsIHBlcm1hbmVudGUgZSBpcnJldm9nw6F2ZWwgZG9zIGRpcmVpdG9zIGF1dG9yYWlzIHBhdHJpbW9uaWFpcyBuw6NvIGNvbWVyY2lhaXMgZGUgdXRpbGl6YcOnw6NvIApkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwgb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciAKbWVpbyBvdSB2ZcOtY3VsbywgaW5jbHVzaXZlIGVtIFJlcG9zaXTDs3Jpb3MgRGlnaXRhaXMsIGJlbSBjb21vIG9zIGRpcmVpdG9zIGRlIHJlcHJvZHXDp8OjbywgZXhpYmnDp8OjbywgZXhlY3XDp8OjbywgZGVjbGFtYcOnw6NvLCBleHBvc2nDp8OjbywgCmFycXVpdmFtZW50bywgaW5jbHVzw6NvIGVtIGJhbmNvIGRlIGRhZG9zLCBwcmVzZXJ2YcOnw6NvLCBkaWZ1c8OjbywgZGlzdHJpYnVpw6fDo28sIGRpdnVsZ2HDp8OjbywgZW1wcsOpc3RpbW8sIHRyYWR1w6fDo28sIGluY2x1c8OjbyBlbSBub3ZhcyAKb2JyYXMgb3UgY29sZXTDom5lYXMsIHJldXRpbGl6YcOnw6NvLCBlZGnDp8OjbywgcHJvZHXDp8OjbyBkZSBtYXRlcmlhbCBkaWTDoXRpY28gZSBjdXJzb3Mgb3UgcXVhbHF1ZXIgZm9ybWEgZGUgdXRpbGl6YcOnw6NvIG7Do28gY29tZXJjaWFsOwogICAgICAgIApSRUNPTkhFQ0UgcXVlIGEgY2Vzc8OjbyBhcXVpIGVzcGVjaWZpY2FkYSBjb25jZWRlIMOgIEZJT0NSVVogLSBGVU5EQcOHw4NPIE9TV0FMRE8gQ1JVWiBvIGRpcmVpdG8gZGUgYXV0b3JpemFyIHF1YWxxdWVyIHBlc3NvYSDigJMgZsOtc2ljYSAKb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdSBlc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlciAKZmluYWxpZGFkZXMgbsOjbyBjb21lcmNpYWlzOwogICAgICAgIApERUNMQVJBIHF1ZSBhIG9icmEgw6kgY3JpYcOnw6NvIG9yaWdpbmFsIGUgcXVlIMOpIG8gdGl0dWxhciBkb3MgZGlyZWl0b3MgYXF1aSBjZWRpZG9zIGUgYXV0b3JpemFkb3MsIHJlc3BvbnNhYmlsaXphbmRvLXNlIGludGVncmFsbWVudGUgCnBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwgaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIAppbmRlbml6YXIgdGVyY2Vpcm9zIHBvciBkYW5vcywgYmVtIGNvbW8gaW5kZW5pemFyIGUgcmVzc2FyY2lyIMOgIEZJT0NSVVogLSBGVU5EQcOHw4NPIE9TV0FMRE8gQ1JVWiBkZSBldmVudHVhaXMgZGVzcGVzYXMgcXVlIHZpZXJlbSBhIApzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91IGRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIAplIHZpb2xhw6fDtWVzIGRlIGRpcmVpdG9zOwogICAgICAgIApBRklSTUEgcXVlIGNvbmhlY2UgYSBQb2zDrXRpY2EgSW5zdGl0dWNpb25hbCBkZSBBY2Vzc28gQWJlcnRvIGRhIEluc3RpdHVpw6fDo28gZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyAKaW5zdGl0dWNpb25hbCBBUkNBLgo=Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-04T11:26:28Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Transcriptome analysis of gossypium hirsutum flower buds infested by cotton boll weevil (anthonomus grandis) larvae
title Transcriptome analysis of gossypium hirsutum flower buds infested by cotton boll weevil (anthonomus grandis) larvae
spellingShingle Transcriptome analysis of gossypium hirsutum flower buds infested by cotton boll weevil (anthonomus grandis) larvae
Artico, Sinara
Larva
Sequenciamento Transcriptoma
Estresse Biótico
Transcriptome
Laser Capture Microdissection
Gossypium
RNA
Larvae
Transcriptome Sequencing
Biotic Stress
Transcriptoma
Microdissecção e Captura a Laser
Gossypium
RNA
title_short Transcriptome analysis of gossypium hirsutum flower buds infested by cotton boll weevil (anthonomus grandis) larvae
title_full Transcriptome analysis of gossypium hirsutum flower buds infested by cotton boll weevil (anthonomus grandis) larvae
title_fullStr Transcriptome analysis of gossypium hirsutum flower buds infested by cotton boll weevil (anthonomus grandis) larvae
title_full_unstemmed Transcriptome analysis of gossypium hirsutum flower buds infested by cotton boll weevil (anthonomus grandis) larvae
title_sort Transcriptome analysis of gossypium hirsutum flower buds infested by cotton boll weevil (anthonomus grandis) larvae
author Artico, Sinara
author_facet Artico, Sinara
Alves, Marcelo Ribeiro
Oliveira Neto, Osmundo Brilhante
Macedo, Leonardo Lima Pepino de
Silveira, Sylvia
Sa, Maria Fátima Grossi de
Martinelli, Adriana Pinheiro
Ferreira, Marcio Alves
author_role author
author2 Alves, Marcelo Ribeiro
Oliveira Neto, Osmundo Brilhante
Macedo, Leonardo Lima Pepino de
Silveira, Sylvia
Sa, Maria Fátima Grossi de
Martinelli, Adriana Pinheiro
Ferreira, Marcio Alves
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Artico, Sinara
Alves, Marcelo Ribeiro
Oliveira Neto, Osmundo Brilhante
Macedo, Leonardo Lima Pepino de
Silveira, Sylvia
Sa, Maria Fátima Grossi de
Martinelli, Adriana Pinheiro
Ferreira, Marcio Alves
dc.subject.other.pt_BR.fl_str_mv Larva
Sequenciamento Transcriptoma
Estresse Biótico
topic Larva
Sequenciamento Transcriptoma
Estresse Biótico
Transcriptome
Laser Capture Microdissection
Gossypium
RNA
Larvae
Transcriptome Sequencing
Biotic Stress
Transcriptoma
Microdissecção e Captura a Laser
Gossypium
RNA
dc.subject.en.pt_BR.fl_str_mv Transcriptome
Laser Capture Microdissection
Gossypium
RNA
Larvae
Transcriptome Sequencing
Biotic Stress
dc.subject.decs.pt_BR.fl_str_mv Transcriptoma
Microdissecção e Captura a Laser
Gossypium
RNA
description We thank Prof. S.M. Tsai, Cell and Molecular Biology Laboratory, CENA/USP, for maintaining the multiuser LMD facility (FAPESP 2009/53998-3). APM acknowledges CNPq (Grant 310.612/2011-0). The authors gratefully thank Marco A. Takita and Carolina M. Rodrigues for use of the computational structure in the Laboratory of Biotechnology, Center for Citrus Sylvio Moreira, Cordeirópolis-Sao Paulo, Brazil. We thank Julia Lambret-Frotté for help us with the phylogenetic analysis. This work was part of S.A’s Ph. D. research in Genetics at the Department of Genetics of the Universidade Federal do Rio de Janeiro (UFRJ) and was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq; M. Alves-Ferreira: # 306025/2010-8) and Fundação de Amparo à Pesquisa do Rio de Janeiro (FAPERJ).
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-07-01T18:37:30Z
dc.date.available.fl_str_mv 2015-07-01T18:37:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv ARTICO, Sinara et al. Transcriptome analysis of Gossypium hirsutum flower buds infested by cotton boll weevil (Anthonomus grandis) larvae. BMC Genomics, v.15, n.854, p.1-24, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11051
dc.identifier.issn.none.fl_str_mv 1471-2164
dc.identifier.doi.none.fl_str_mv 10.1186/1471-2164-15-854
dc.identifier.eissn.none.fl_str_mv 1471-2164
identifier_str_mv ARTICO, Sinara et al. Transcriptome analysis of Gossypium hirsutum flower buds infested by cotton boll weevil (Anthonomus grandis) larvae. BMC Genomics, v.15, n.854, p.1-24, 2014.
1471-2164
10.1186/1471-2164-15-854
url https://www.arca.fiocruz.br/handle/icict/11051
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC Genomics
publisher.none.fl_str_mv BMC Genomics
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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