Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy.

Vasconcelos, Juliana Fraga; Souza, Bruno Solano de Freitas; Lins, Thayse Fernanda da Silva; Garcia, Letícia Maria da Silva; Kaneto, Carla Martins; Sampaio, Geraldo Pedral; Alcantara, Adriano Costa de; Meira, Cássio Santana; Macambira, Simone Garcia; Santos, Ricardo Ribeiro dos; Soares, Milena Botelho Pereira

Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy.

Detalhes bibliográficos
Autor(a) principal:	Vasconcelos, Juliana Fraga
Data de Publicação:	2013
Outros Autores:	Souza, Bruno Solano de Freitas, Lins, Thayse Fernanda da Silva, Garcia, Letícia Maria da Silva, Kaneto, Carla Martins, Sampaio, Geraldo Pedral, Alcantara, Adriano Costa de, Meira, Cássio Santana, Macambira, Simone Garcia, Santos, Ricardo Ribeiro dos, Soares, Milena Botelho Pereira
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/8571
Resumo:	Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil

Metadados do item

id	CRUZ_7124e31f492a798e7927cf026f2c4122
oai_identifier_str	oai:www.arca.fiocruz.br:icict/8571
network_acronym_str	CRUZ
network_name_str	Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str	2135
spelling	Vasconcelos, Juliana FragaSouza, Bruno Solano de FreitasLins, Thayse Fernanda da SilvaGarcia, Letícia Maria da SilvaKaneto, Carla MartinsSampaio, Geraldo PedralAlcantara, Adriano Costa deMeira, Cássio SantanaMacambira, Simone GarciaSantos, Ricardo Ribeiro dosSoares, Milena Botelho Pereira2014-10-09T18:48:31Z2014-10-09T18:48:31Z2013VASCONCELOS, J. F. et al. Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy. FASEB Journal, v. 27, n. 12, p. 4691-4702, 2013.1530-6860https://www.arca.fiocruz.br/handle/icict/857110.1096/fj.13-229351engFederation of American Society of Experimental BiologyAdministration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Universidade Estadual de Santa Cruz. Ilhéus, BA, BrasilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasilospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilChagas disease, caused by Trypanosoma cruzi infection, is a leading cause of heart failure in Latin American countries. In a previous study, we showed beneficial effects of granulocyte colony-stimulating factor (G-CSF) administration in the heart function of mice with chronic T. cruzi infection. Presently, we investigated the mechanisms by which this cytokine exerts its beneficial effects. Mice chronically infected with T. cruzi were treated with human recombinant G-CSF (3 courses of 200 µg/kg/d for 5 d). Inflammation and fibrosis were reduced in the hearts of G-CSF-treated mice, compared with the hearts of vehicle-treated mice, which correlated with decreased syndecan-4, intercellular adhesion molecule-1, and galectin-3 expressions. Marked reductions in interferon-Î³ and tumor necrosis factor-Î± and increased interleukin-10 and transforming growth factor-ß were found after G-CSF administration. Because the therapy did not induce a Th1 to Th2 immune response deviation, we investigated the role of regulatory T (Treg) cells. A significant increase in CD3(+)Foxp3(+) cells was observed in the hearts of G-CSF-treated mice. In addition, a reduction of parasitism was observed after G-CSF treatment. Our results indicate a role of Treg cells in the immunosuppression induced by G-CSF treatment and reinforces its potential therapeutic use for patients with Chagas diseaseTrypanosoma cruziCytokine therapyInflammationFibrosisTh1 modulationCardiomiopatia Chagásica/quimioterapiaFator Estimulador de Colônias de Granulócitos/uso terapêuticoImunomodulaçãoMiocardite/quimioterapiaLinfócitos T Reguladores/efeitos de drogasAnimaisAntígenos CD3/genéticaCardiomiopatia Chagásica/imunologiaCitocinas/genéticaFibrose/quimioterapiaFatores de Transcrição Forkhead/genéticaGalectina 3/genéticaFator Estimulador de Colônias de Granulócitos/administração & dosagemHumanosInjeções IntraperitoneaisMolécula 1 de Adesão Intercelular/genéticaCamundongos Endogâmicos C57BLMiocárdio/imunologiaSindecana-4/genéticaTranscrição GenéticaTrypanosoma cruzi/efeitos de drogasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8571/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALVasconcelos JF Administration....pdfVasconcelos JF Administration....pdfapplication/pdf1028504https://www.arca.fiocruz.br/bitstream/icict/8571/2/Vasconcelos%20JF%20Administration....pdfcf15c623e7c8b011509bf0ed3eb8c71bMD52TEXTVasconcelos JF Administration....pdf.txtVasconcelos JF Administration....pdf.txtExtracted texttext/plain57848https://www.arca.fiocruz.br/bitstream/icict/8571/3/Vasconcelos%20JF%20Administration....pdf.txtd8b9b061a75ef4a3c0a48a048e124697MD53icict/85712023-03-15 14:33:24.908oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:24Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv	Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy.
title	Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy.
spellingShingle	Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy. Vasconcelos, Juliana Fraga Trypanosoma cruzi Cytokine therapy Inflammation Fibrosis Th1 modulation Cardiomiopatia Chagásica/quimioterapia Fator Estimulador de Colônias de Granulócitos/uso terapêutico Imunomodulação Miocardite/quimioterapia Linfócitos T Reguladores/efeitos de drogas Animais Antígenos CD3/genética Cardiomiopatia Chagásica/imunologia Citocinas/genética Fibrose/quimioterapia Fatores de Transcrição Forkhead/genética Galectina 3/genética Fator Estimulador de Colônias de Granulócitos/administração & dosagem Humanos Injeções Intraperitoneais Molécula 1 de Adesão Intercelular/genética Camundongos Endogâmicos C57BL Miocárdio/imunologia Sindecana-4/genética Transcrição Genética Trypanosoma cruzi/efeitos de drogas
title_short	Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy.
title_full	Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy.
title_fullStr	Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy.
title_full_unstemmed	Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy.
title_sort	Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy.
author	Vasconcelos, Juliana Fraga
author_facet	Vasconcelos, Juliana Fraga Souza, Bruno Solano de Freitas Lins, Thayse Fernanda da Silva Garcia, Letícia Maria da Silva Kaneto, Carla Martins Sampaio, Geraldo Pedral Alcantara, Adriano Costa de Meira, Cássio Santana Macambira, Simone Garcia Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira
author_role	author
author2	Souza, Bruno Solano de Freitas Lins, Thayse Fernanda da Silva Garcia, Letícia Maria da Silva Kaneto, Carla Martins Sampaio, Geraldo Pedral Alcantara, Adriano Costa de Meira, Cássio Santana Macambira, Simone Garcia Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira
author2_role	author author author author author author author author author author
dc.contributor.author.fl_str_mv	Vasconcelos, Juliana Fraga Souza, Bruno Solano de Freitas Lins, Thayse Fernanda da Silva Garcia, Letícia Maria da Silva Kaneto, Carla Martins Sampaio, Geraldo Pedral Alcantara, Adriano Costa de Meira, Cássio Santana Macambira, Simone Garcia Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira
dc.subject.en.pt_BR.fl_str_mv	Trypanosoma cruzi Cytokine therapy Inflammation Fibrosis Th1 modulation
topic	Trypanosoma cruzi Cytokine therapy Inflammation Fibrosis Th1 modulation Cardiomiopatia Chagásica/quimioterapia Fator Estimulador de Colônias de Granulócitos/uso terapêutico Imunomodulação Miocardite/quimioterapia Linfócitos T Reguladores/efeitos de drogas Animais Antígenos CD3/genética Cardiomiopatia Chagásica/imunologia Citocinas/genética Fibrose/quimioterapia Fatores de Transcrição Forkhead/genética Galectina 3/genética Fator Estimulador de Colônias de Granulócitos/administração & dosagem Humanos Injeções Intraperitoneais Molécula 1 de Adesão Intercelular/genética Camundongos Endogâmicos C57BL Miocárdio/imunologia Sindecana-4/genética Transcrição Genética Trypanosoma cruzi/efeitos de drogas
dc.subject.decs.pt_BR.fl_str_mv	Cardiomiopatia Chagásica/quimioterapia Fator Estimulador de Colônias de Granulócitos/uso terapêutico Imunomodulação Miocardite/quimioterapia Linfócitos T Reguladores/efeitos de drogas Animais Antígenos CD3/genética Cardiomiopatia Chagásica/imunologia Citocinas/genética Fibrose/quimioterapia Fatores de Transcrição Forkhead/genética Galectina 3/genética Fator Estimulador de Colônias de Granulócitos/administração & dosagem Humanos Injeções Intraperitoneais Molécula 1 de Adesão Intercelular/genética Camundongos Endogâmicos C57BL Miocárdio/imunologia Sindecana-4/genética Transcrição Genética Trypanosoma cruzi/efeitos de drogas
description	Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
publishDate	2013
dc.date.issued.fl_str_mv	2013
dc.date.accessioned.fl_str_mv	2014-10-09T18:48:31Z
dc.date.available.fl_str_mv	2014-10-09T18:48:31Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	VASCONCELOS, J. F. et al. Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy. FASEB Journal, v. 27, n. 12, p. 4691-4702, 2013.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/8571
dc.identifier.issn.none.fl_str_mv	1530-6860
dc.identifier.doi.none.fl_str_mv	10.1096/fj.13-229351
identifier_str_mv	VASCONCELOS, J. F. et al. Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy. FASEB Journal, v. 27, n. 12, p. 4691-4702, 2013. 1530-6860 10.1096/fj.13-229351
url	https://www.arca.fiocruz.br/handle/icict/8571
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Federation of American Society of Experimental Biology
publisher.none.fl_str_mv	Federation of American Society of Experimental Biology
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/8571/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/8571/2/Vasconcelos%20JF%20Administration....pdf https://www.arca.fiocruz.br/bitstream/icict/8571/3/Vasconcelos%20JF%20Administration....pdf.txt
bitstream.checksum.fl_str_mv	7d48279ffeed55da8dfe2f8e81f3b81f cf15c623e7c8b011509bf0ed3eb8c71b d8b9b061a75ef4a3c0a48a048e124697
bitstream.checksumAlgorithm.fl_str_mv	MD5 MD5 MD5
repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv	repositorio.arca@fiocruz.br
_version_	1813009105839718400

Administration of granulocyte colony-stimulating factor induces immunomodulation, recruitment of T regulatory cells, reduction of myocarditis and decrease of parasite load in a mouse model of chronic Chagas disease cardiomyopathy.

Registros relacionados