Development of a multiplex bead-based assay for detection of Hepatitis C virus
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/28739 |
Resumo: | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil. |
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Fonseca, Bruna P. F.Marques, Christiane F. S.Nascimento, Lílian D.Mello, Marcelle B.Silva, Leila B. R.Rubim, Nara M.Foti, LeonardoSilva, Edimilson Domingos daFerreira, Antônio Gomes PintoKrieger, Marco Aurélio2018-09-13T14:21:41Z2018-09-13T14:21:41Z2011FONSECA, Bruna P. F. et al. Development of a multiplex bead-based assay for detection of Hepatitis C virus. Clinical and Vaccine Immunology, v. 18, n. 5, p. 802-806, 3 May 2011.1556-6811https://www.arca.fiocruz.br/handle/icict/2873910.1128/CVI.00265-101556-679XporAmerican Society for MicrobiologyHepacivirusHepatitis CHepatitis AntigensReagent KitsDiagnosticAntígenos de la HepatitisJuego de Reactivos para DiagnósticoHepatite CAntígenos de HepatiteKit de Reagentes para DiagnósticoDevelopment of a multiplex bead-based assay for detection of Hepatitis C virusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Hepatitis C virus (HCV) infection is a major burden to public health worldwide, affecting approximately 3% of the human population. Although HCV detection is currently based on reliable tests, the field of medical diagnostics has a growing need for inexpensive, accurate, and quick high-throughput assays. By using the recombinant HCV antigens NS3, NS4, NS5, and Combined, we describe a new bead-based multiplex test capable of detecting HCV infection in human serum samples. The first analysis, made in a singleplex format, showed that each antigen coupled to an individual bead set presented high-level responses for anti-HCV-positive reference serum pools and lower-level responses for the HCV-negative pools. Our next approach was to determine the sensitivity and specificity of each antigen by testing 93 HCV-positive and 93 HCV-negative sera. When assayed in the singleplex format, the NS3, NS4, and NS5 antigens presented lower sensitivity values (50.5%, 51.6%, and 55.9%, respectively) than did the Combined antigen, which presented a sensitivity of 93.5%. All antigens presented 100% specificity. These antigens were then multiplexed in a 4-plex assay, which resulted in increased sensitivity and specificity values, performing with 100% sensitivity and 100% specificity. The positive and negative predictive values for the 4-plex assay were 100%. Although preliminary, this 4-plex assay showed robust results that, aligned with its small-sample-volume requirements and also its cost- and time-effectiveness, make it a reasonable alternative to tests currently used for HCV screening of potentially infected individuals.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/28739/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALClinVacImuncd802.pdfClinVacImuncd802.pdfapplication/pdf625084https://www.arca.fiocruz.br/bitstream/icict/28739/2/ClinVacImuncd802.pdfdf031f6f40c0f22a547b8b8b2c3306deMD52TEXTClinVacImuncd802.pdf.txtClinVacImuncd802.pdf.txtExtracted texttext/plain29262https://www.arca.fiocruz.br/bitstream/icict/28739/3/ClinVacImuncd802.pdf.txt12a27d0227179115c87aae94141f9beaMD53icict/287392022-04-04 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| dc.title.en_US.fl_str_mv |
Development of a multiplex bead-based assay for detection of Hepatitis C virus |
| title |
Development of a multiplex bead-based assay for detection of Hepatitis C virus |
| spellingShingle |
Development of a multiplex bead-based assay for detection of Hepatitis C virus Fonseca, Bruna P. F. Hepacivirus Hepatitis C Hepatitis Antigens Reagent Kits Diagnostic Antígenos de la Hepatitis Juego de Reactivos para Diagnóstico Hepatite C Antígenos de Hepatite Kit de Reagentes para Diagnóstico |
| title_short |
Development of a multiplex bead-based assay for detection of Hepatitis C virus |
| title_full |
Development of a multiplex bead-based assay for detection of Hepatitis C virus |
| title_fullStr |
Development of a multiplex bead-based assay for detection of Hepatitis C virus |
| title_full_unstemmed |
Development of a multiplex bead-based assay for detection of Hepatitis C virus |
| title_sort |
Development of a multiplex bead-based assay for detection of Hepatitis C virus |
| author |
Fonseca, Bruna P. F. |
| author_facet |
Fonseca, Bruna P. F. Marques, Christiane F. S. Nascimento, Lílian D. Mello, Marcelle B. Silva, Leila B. R. Rubim, Nara M. Foti, Leonardo Silva, Edimilson Domingos da Ferreira, Antônio Gomes Pinto Krieger, Marco Aurélio |
| author_role |
author |
| author2 |
Marques, Christiane F. S. Nascimento, Lílian D. Mello, Marcelle B. Silva, Leila B. R. Rubim, Nara M. Foti, Leonardo Silva, Edimilson Domingos da Ferreira, Antônio Gomes Pinto Krieger, Marco Aurélio |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Fonseca, Bruna P. F. Marques, Christiane F. S. Nascimento, Lílian D. Mello, Marcelle B. Silva, Leila B. R. Rubim, Nara M. Foti, Leonardo Silva, Edimilson Domingos da Ferreira, Antônio Gomes Pinto Krieger, Marco Aurélio |
| dc.subject.other.pt_BR.fl_str_mv |
Hepacivirus |
| topic |
Hepacivirus Hepatitis C Hepatitis Antigens Reagent Kits Diagnostic Antígenos de la Hepatitis Juego de Reactivos para Diagnóstico Hepatite C Antígenos de Hepatite Kit de Reagentes para Diagnóstico |
| dc.subject.en.en_US.fl_str_mv |
Hepatitis C Hepatitis Antigens Reagent Kits Diagnostic |
| dc.subject.es.es_ES.fl_str_mv |
Antígenos de la Hepatitis Juego de Reactivos para Diagnóstico |
| dc.subject.decs.pt_BR.fl_str_mv |
Hepatite C Antígenos de Hepatite Kit de Reagentes para Diagnóstico |
| description |
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia Diagnóstica. Rio de Janeiro, RJ, Brasil. |
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2011 |
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2011 |
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2018-09-13T14:21:41Z |
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2018-09-13T14:21:41Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
FONSECA, Bruna P. F. et al. Development of a multiplex bead-based assay for detection of Hepatitis C virus. Clinical and Vaccine Immunology, v. 18, n. 5, p. 802-806, 3 May 2011. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/28739 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1556-6811 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1128/CVI.00265-10 |
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1556-679X |
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FONSECA, Bruna P. F. et al. Development of a multiplex bead-based assay for detection of Hepatitis C virus. Clinical and Vaccine Immunology, v. 18, n. 5, p. 802-806, 3 May 2011. 1556-6811 10.1128/CVI.00265-10 1556-679X |
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American Society for Microbiology |
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American Society for Microbiology |
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