Genetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp.

Detalhes bibliográficos
Autor(a) principal: McBride, Alan John Alexander
Data de Publicação: 2008
Outros Autores: Cerqueira, Gustavo Maia de, Suchard, Marc A, Moreira, Ângela N, Zuerner, Richard L, Reis, Mitermayer Galvão dos, Haake, David A, Ko, Albert Icksang, Dellagostin, Odir Antonio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7536
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
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spelling McBride, Alan John AlexanderCerqueira, Gustavo Maia deSuchard, Marc AMoreira, Ângela NZuerner, Richard LReis, Mitermayer Galvão dosHaake, David AKo, Albert IcksangDellagostin, Odir Antonio2014-04-25T11:49:23Z2014-04-25T11:49:23Z2008McBRIDE, A. J. A. et al. Genetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp. Infections, Genetics and Evolution, v. 9, n. 2, p. 196-205, 2009.1567-7257https://www.arca.fiocruz.br/handle/icict/753610.1016/j.meegid.2008.10.012.engNational Institute of HealthGenetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, BrasilDavid Geffen School of Medicine at UCLA. Department of Biomathematics. Department of Human Genetics. Department of Biostatistics. Los Angeles, CA, USAUniversidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, BrasilNational Animal Disease Centre. Agricultural Research Service. U.S. Department of Agriculture Ames. Iowa, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilVeterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, USA / David Geffen School of Medicine at UCLA. Department of Medicine. Los Angeles, CA, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Disease. Weill Medical College of Cornell University. New York, NY, USAUniversidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, BrasilRecent serologic, immunoprotection, and pathogenesis studies identified the Lig proteins as key virulence determinants in interactions of leptospiral pathogens with the mammalian host. We examined the sequence variation and recombination patterns of ligA, ligB, and ligC among 10 pathogenic strains from five Leptospira species. All strains were found to have intact ligB genes and genetic drift accounting for most of the ligB genetic diversity observed. The ligA gene was found exclusively in L. interrogans and L. kirschneri strains, and was created from ligB by a two-step partial gene duplication process. The aminoterminal domain of LigB and the LigA paralog were essentially identical (98.5 ± 0.8% mean identity) in strains with both genes. Like ligB, ligC gene variation also followed phylogenetic patterns, suggesting an early gene duplication event. However, ligC is a pseudogene in several strains, suggesting that LigC is not essential for virulence. Two ligB genes and one ligC gene had mosaic compositions and evidence for recombination events between related Leptospira species was also found for some ligA genes. In conclusion, the results presented here indicate that Lig diversity has important ramifications for the selection of Lig polypeptides for use in diagnosis and as vaccine candidates. This sequence information will aid the identification of highly conserved regions within the Lig proteins and improve upon the performance characteristics of the Lig proteins in diagnostic assays and in subunit vaccine formulations with the potential to confer heterologous protection.LeptospirosisLigPathogenesisMolecular evolutionSequence analysisAntígenos de Bactérias/genéticaDNA Bacteriano/genéticaVariação GenéticaAnimaisLeptospira/fisiologiaLeptospira/genéticaLeptospira/imunologiaLeptospirose/imunologiaDados de Sequência MolecularFilogeniaAnálise de Sequência de DNAAnálise de Sequência de Proteínainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALMcBRIDE AJA Genetic diversity....pdfMcBRIDE AJA Genetic diversity....pdfapplication/pdf1689793https://www.arca.fiocruz.br/bitstream/icict/7536/1/McBRIDE%20AJA%20Genetic%20diversity....pdf2a4fa33223fdb336d7d432d7c2165bcaMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7536/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTMcBRIDE AJA Genetic diversity....pdf.txtMcBRIDE AJA Genetic diversity....pdf.txtExtracted texttext/plain68564https://www.arca.fiocruz.br/bitstream/icict/7536/5/McBRIDE%20AJA%20Genetic%20diversity....pdf.txt811f3ff1da3ee96d646bf2ca20136314MD55THUMBNAILMcBRIDE AJA Genetic diversity....pdf.jpgMcBRIDE AJA Genetic diversity....pdf.jpgGenerated Thumbnailimage/jpeg2311https://www.arca.fiocruz.br/bitstream/icict/7536/4/McBRIDE%20AJA%20Genetic%20diversity....pdf.jpgda8bd9d4f14dc956bea2aafcbb09d165MD54icict/75362023-03-15 14:33:17.857oai:www.arca.fiocruz.br:icict/7536TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:17Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Genetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp.
title Genetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp.
spellingShingle Genetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp.
McBride, Alan John Alexander
Leptospirosis
Lig
Pathogenesis
Molecular evolution
Sequence analysis
Antígenos de Bactérias/genética
DNA Bacteriano/genética
Variação Genética
Animais
Leptospira/fisiologia
Leptospira/genética
Leptospira/imunologia
Leptospirose/imunologia
Dados de Sequência Molecular
Filogenia
Análise de Sequência de DNA
Análise de Sequência de Proteína
title_short Genetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp.
title_full Genetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp.
title_fullStr Genetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp.
title_full_unstemmed Genetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp.
title_sort Genetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp.
author McBride, Alan John Alexander
author_facet McBride, Alan John Alexander
Cerqueira, Gustavo Maia de
Suchard, Marc A
Moreira, Ângela N
Zuerner, Richard L
Reis, Mitermayer Galvão dos
Haake, David A
Ko, Albert Icksang
Dellagostin, Odir Antonio
author_role author
author2 Cerqueira, Gustavo Maia de
Suchard, Marc A
Moreira, Ângela N
Zuerner, Richard L
Reis, Mitermayer Galvão dos
Haake, David A
Ko, Albert Icksang
Dellagostin, Odir Antonio
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv McBride, Alan John Alexander
Cerqueira, Gustavo Maia de
Suchard, Marc A
Moreira, Ângela N
Zuerner, Richard L
Reis, Mitermayer Galvão dos
Haake, David A
Ko, Albert Icksang
Dellagostin, Odir Antonio
dc.subject.en.pt_BR.fl_str_mv Leptospirosis
Lig
Pathogenesis
Molecular evolution
Sequence analysis
topic Leptospirosis
Lig
Pathogenesis
Molecular evolution
Sequence analysis
Antígenos de Bactérias/genética
DNA Bacteriano/genética
Variação Genética
Animais
Leptospira/fisiologia
Leptospira/genética
Leptospira/imunologia
Leptospirose/imunologia
Dados de Sequência Molecular
Filogenia
Análise de Sequência de DNA
Análise de Sequência de Proteína
dc.subject.decs.pt_BR.fl_str_mv Antígenos de Bactérias/genética
DNA Bacteriano/genética
Variação Genética
Animais
Leptospira/fisiologia
Leptospira/genética
Leptospira/imunologia
Leptospirose/imunologia
Dados de Sequência Molecular
Filogenia
Análise de Sequência de DNA
Análise de Sequência de Proteína
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2014-04-25T11:49:23Z
dc.date.available.fl_str_mv 2014-04-25T11:49:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv McBRIDE, A. J. A. et al. Genetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp. Infections, Genetics and Evolution, v. 9, n. 2, p. 196-205, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7536
dc.identifier.issn.none.fl_str_mv 1567-7257
dc.identifier.doi.none.fl_str_mv 10.1016/j.meegid.2008.10.012.
identifier_str_mv McBRIDE, A. J. A. et al. Genetic diversity of the Leptospiral immunoglobulin-like (Lig) genes in pathogenic Leptospira spp. Infections, Genetics and Evolution, v. 9, n. 2, p. 196-205, 2009.
1567-7257
10.1016/j.meegid.2008.10.012.
url https://www.arca.fiocruz.br/handle/icict/7536
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv National Institute of Health
publisher.none.fl_str_mv National Institute of Health
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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