Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.

Cerqueira, Gustavo Maia de; McBride, Alan John Alexander; Picardeau, Mathieu; Ribeiro, Samuel G; Moreira, Ângela N; Morel, Viviane; Reis, Mitermayer Galvão dos; Ko, Albert Icksang; Dellagostin, Odir Antonio

Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.

Detalhes bibliográficos
Autor(a) principal:	Cerqueira, Gustavo Maia de
Data de Publicação:	2009
Outros Autores:	McBride, Alan John Alexander, Picardeau, Mathieu, Ribeiro, Samuel G, Moreira, Ângela N, Morel, Viviane, Reis, Mitermayer Galvão dos, Ko, Albert Icksang, Dellagostin, Odir Antonio
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/7520
Resumo:	Federal University of Pelotas. Biotechnology Centre. Pelotas, RS, Brasil / Institut Pasteur. Unité de Biologie des Spirochètes. Paris, France

Metadados do item

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spelling	Cerqueira, Gustavo Maia deMcBride, Alan John AlexanderPicardeau, MathieuRibeiro, Samuel GMoreira, Ângela NMorel, VivianeReis, Mitermayer Galvão dosKo, Albert IcksangDellagostin, Odir Antonio2014-04-14T18:30:01Z2014-04-14T18:30:01Z2009CERQUEIRA, G. M. et al. Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates. Journal of Medical Microbiology, v. 58, n. 9, p. 1173–1181, 2009.0022-2615https://www.arca.fiocruz.br/handle/icict/752010.1099/jmm.0.009175-0engSGMDistribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Pelotas. Biotechnology Centre. Pelotas, RS, Brasil / Institut Pasteur. Unité de Biologie des Spirochètes. Paris, FranceFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilInstitut Pasteur. Unité de Biologie des Spirochètes. Paris, FranceFederal University of Pelotas. Biotechnology Centre. Pelotas, RS, BrasilFederal University of Pelotas. Biotechnology Centre. Pelotas, RS, BrasilInstitut Pasteur. Unité de Biologie des Spirochètes. Paris, FranceFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Disease. Weill Medical College of Cornell University. New York, NY, USAFederal University of Pelotas. Biotechnology Centre. Pelotas, RS, BrasilThe family of leptospiral immunoglobulin-like (lig) genes comprises ligA, ligB and ligC. This study used PCR to demonstrate the presence of lig genes among serovars from a collection of leptospiral strains and clinical isolates. Whilst ligA and ligC appeared to be present in a limited number of pathogenic serovars, the ligB gene was distributed ubiquitously among all pathogenic strains. None of the lig genes were detected among intermediate or saprophytic Leptospira species. It was also shown that, similar to the previously characterized secY gene, a short specific PCR fragment of ligB could be used to correctly identify pathogenic Leptospira species. These findings demonstrate that ligB is widely present among pathogenic strains and may be useful for their reliable identification and classification.Antígenos de Bactérias/genéticaLeptospira/classificaçãoLeptospira/genéticaAntígenos de Bactérias/químicaAntígenos de Bactérias/imunologiaTécnicas de Tipagem BacterianaSequência de BasesDNA Bacteriano/genéticaRegulação Bacteriana da Expressão Gênica/fisiologiaLeptospira/imunologiaLeptospira/patogenicidadeDados de Sequência MolecularFilogeniaRNA Bacteriano/genéticainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALCerqueira GM Distribution of the leptospiral....pdfCerqueira GM Distribution of the leptospiral....pdfapplication/pdf198731https://www.arca.fiocruz.br/bitstream/icict/7520/1/Cerqueira%20GM%20Distribution%20of%20the%20leptospiral....pdf7a40d1f6d1574e942d3bbefd3ce063d4MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7520/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTCerqueira GM Distribution of the leptospiral....pdf.txtCerqueira GM Distribution of the leptospiral....pdf.txtExtracted texttext/plain39822https://www.arca.fiocruz.br/bitstream/icict/7520/5/Cerqueira%20GM%20Distribution%20of%20the%20leptospiral....pdf.txtcce97d5fe3b270e30fb1cb4b785c5029MD55THUMBNAILCerqueira GM Distribution of the leptospiral....pdf.jpgCerqueira GM Distribution of the leptospiral....pdf.jpgGenerated Thumbnailimage/jpeg2475https://www.arca.fiocruz.br/bitstream/icict/7520/4/Cerqueira%20GM%20Distribution%20of%20the%20leptospiral....pdf.jpga40efcc76798c20496d5b45c9c8b1456MD54icict/75202023-03-15 14:33:17.808oai:www.arca.fiocruz.br:icict/7520TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:17Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv	Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.
title	Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.
spellingShingle	Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates. Cerqueira, Gustavo Maia de Antígenos de Bactérias/genética Leptospira/classificação Leptospira/genética Antígenos de Bactérias/química Antígenos de Bactérias/imunologia Técnicas de Tipagem Bacteriana Sequência de Bases DNA Bacteriano/genética Regulação Bacteriana da Expressão Gênica/fisiologia Leptospira/imunologia Leptospira/patogenicidade Dados de Sequência Molecular Filogenia RNA Bacteriano/genética
title_short	Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.
title_full	Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.
title_fullStr	Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.
title_full_unstemmed	Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.
title_sort	Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.
author	Cerqueira, Gustavo Maia de
author_facet	Cerqueira, Gustavo Maia de McBride, Alan John Alexander Picardeau, Mathieu Ribeiro, Samuel G Moreira, Ângela N Morel, Viviane Reis, Mitermayer Galvão dos Ko, Albert Icksang Dellagostin, Odir Antonio
author_role	author
author2	McBride, Alan John Alexander Picardeau, Mathieu Ribeiro, Samuel G Moreira, Ângela N Morel, Viviane Reis, Mitermayer Galvão dos Ko, Albert Icksang Dellagostin, Odir Antonio
author2_role	author author author author author author author author
dc.contributor.author.fl_str_mv	Cerqueira, Gustavo Maia de McBride, Alan John Alexander Picardeau, Mathieu Ribeiro, Samuel G Moreira, Ângela N Morel, Viviane Reis, Mitermayer Galvão dos Ko, Albert Icksang Dellagostin, Odir Antonio
dc.subject.decs.pt_BR.fl_str_mv	Antígenos de Bactérias/genética Leptospira/classificação Leptospira/genética Antígenos de Bactérias/química Antígenos de Bactérias/imunologia Técnicas de Tipagem Bacteriana Sequência de Bases DNA Bacteriano/genética Regulação Bacteriana da Expressão Gênica/fisiologia Leptospira/imunologia Leptospira/patogenicidade Dados de Sequência Molecular Filogenia RNA Bacteriano/genética
topic	Antígenos de Bactérias/genética Leptospira/classificação Leptospira/genética Antígenos de Bactérias/química Antígenos de Bactérias/imunologia Técnicas de Tipagem Bacteriana Sequência de Bases DNA Bacteriano/genética Regulação Bacteriana da Expressão Gênica/fisiologia Leptospira/imunologia Leptospira/patogenicidade Dados de Sequência Molecular Filogenia RNA Bacteriano/genética
description	Federal University of Pelotas. Biotechnology Centre. Pelotas, RS, Brasil / Institut Pasteur. Unité de Biologie des Spirochètes. Paris, France
publishDate	2009
dc.date.issued.fl_str_mv	2009
dc.date.accessioned.fl_str_mv	2014-04-14T18:30:01Z
dc.date.available.fl_str_mv	2014-04-14T18:30:01Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	CERQUEIRA, G. M. et al. Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates. Journal of Medical Microbiology, v. 58, n. 9, p. 1173–1181, 2009.
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/7520
dc.identifier.issn.none.fl_str_mv	0022-2615
dc.identifier.doi.none.fl_str_mv	10.1099/jmm.0.009175-0
identifier_str_mv	CERQUEIRA, G. M. et al. Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates. Journal of Medical Microbiology, v. 58, n. 9, p. 1173–1181, 2009. 0022-2615 10.1099/jmm.0.009175-0
url	https://www.arca.fiocruz.br/handle/icict/7520
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	SGM
publisher.none.fl_str_mv	SGM
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
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Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.

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