Critical role of ROR t in a new thymic pathway leading to IL-17-producing invariant NKT cell differentiation

Detalhes bibliográficos
Autor(a) principal: Michel, Marie-Laure
Data de Publicação: 2008
Outros Autores: Cruz, Daniella Mendes da, Keller, Alexandre Castro, Lochner, Matthias, Schneider, Elke, Dy, Michel, Ebert, Gérard, Moraes, Maria C. Leite de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/39842
Resumo: Université Paris Descartes. Faculté de Médecine. Centre National de la Recherche Scientifique. Unité Mixte de Recherche 8147. Hôpital Necker. Paris, France.
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spelling Michel, Marie-LaureCruz, Daniella Mendes daKeller, Alexandre CastroLochner, MatthiasSchneider, ElkeDy, MichelEbert, GérardMoraes, Maria C. Leite de2020-02-11T14:08:55Z2020-02-11T14:08:55Z2008MICHEL, Marie-Laure et al. Critical role of ROR t in a new thymic pathway leading to IL-17-producing invariant NKT cell differentiation. PNAS, v; 105. n. 50, p. 19845-19850, Dec. 2008.0027-8424https://www.arca.fiocruz.br/handle/icict/3984210.1111/j.1472-765X.2008.02322.x1091-6490engNational Academy of SciencesCélulas T Matadoras NaturaisCitocinasThymic precursors-GalCerCD1dCytokinesCritical role of ROR t in a new thymic pathway leading to IL-17-producing invariant NKT cell differentiationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversité Paris Descartes. Faculté de Médecine. Centre National de la Recherche Scientifique. Unité Mixte de Recherche 8147. Hôpital Necker. Paris, France.Université Paris Descartes. Faculté de Médecine. Centre National de la Recherche Scientifique. Unité Mixte de Recherche 8147. Hôpital Necker. Paris, France / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.Université Paris Descartes. Faculté de Médecine. Centre National de la Recherche Scientifique. Unité Mixte de Recherche 8147. Hôpital Necker. Paris, France / University Federal de Sao Paulo. São Paulo, SP, Brasil.Institut Pasteur. Centre National de la Recherche Scientifique. Unité de Recherche Associée 1961. Laboratory of Lymphoid Tissue Development. Paris, France.Université Paris Descartes. Faculté de Médecine. Centre National de la Recherche Scientifique. Unité Mixte de Recherche 8147. Hôpital Necker. Paris, France.Université Paris Descartes. Faculté de Médecine. Centre National de la Recherche Scientifique. Unité Mixte de Recherche 8147. Hôpital Necker. Paris, France.Institut Pasteur. Centre National de la Recherche Scientifique. Unité de Recherche Associée 1961. Laboratory of Lymphoid Tissue Development. Paris, France.Université Paris Descartes. Faculté de Médecine. Centre National de la Recherche Scientifique. Unité Mixte de Recherche 8147. Hôpital Necker. Paris, France.Invariant natural killer T (iNKT) cells constitute a subpopulation of T cells that recognize glycolipids presented by CD1d molecules. They are characterized by their prompt production of interleukin-4 (IL-4) and interferon- (IFN- ), which enables them to modulate diverse immune responses. Recently, we enlarged this concept by identifying a distinct IL-17-producing iNKT cell subset, named iNKT17 cells. The mechanisms leading to the acquisition of this new iNKT cell activity are unknown. Herein we show that IL-17-producing iNKT cells are already present in the thymus, predominantly among a subset regarded so far as an immature stage of thymic iNKT cell development, the CD1d tetramerposCD44posNK1.1negCD4neg cells. Using EGFP reporter mice, we demonstrate that the transcription factor ROR- t is critical for the thymic differentiation of this subset because only ROR- tpos iNKT cells are capable of massively secreting IL-17. Moreover, IL-17-producing CD1d tetramerposCD44posNK1.1negCD4neg thymic iNKT cells have reached a mature differentiation stage because they fail to generate other cell subsets in fetal thymic organ culture. Conversely, thymic ROR- tneg iNKT cell precursors give rise to progeny, but acquire neither ROR- t expression nor the ability to secrete IL-17. In conclusion, our findings demonstrate an alternative thymic pathway leading to the development of iNKT17 cells that requires ROR- t expression.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/39842/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDanielaMendes_Cruz_etal_IOC_2008.pdfDanielaMendes_Cruz_etal_IOC_2008.pdfapplication/pdf1638005https://www.arca.fiocruz.br/bitstream/icict/39842/2/DanielaMendes_Cruz_etal_IOC_2008.pdf617b2380d2cc526608333882b19aab2bMD52TEXTDanielaMendes_Cruz_etal_IOC_2008.pdf.txtDanielaMendes_Cruz_etal_IOC_2008.pdf.txtExtracted texttext/plain35866https://www.arca.fiocruz.br/bitstream/icict/39842/3/DanielaMendes_Cruz_etal_IOC_2008.pdf.txt450c666c77495ea806634af376604dedMD53icict/398422020-04-09 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dc.title.pt_BR.fl_str_mv Critical role of ROR t in a new thymic pathway leading to IL-17-producing invariant NKT cell differentiation
title Critical role of ROR t in a new thymic pathway leading to IL-17-producing invariant NKT cell differentiation
spellingShingle Critical role of ROR t in a new thymic pathway leading to IL-17-producing invariant NKT cell differentiation
Michel, Marie-Laure
Células T Matadoras Naturais
Citocinas
Thymic precursors
-GalCer
CD1d
Cytokines
title_short Critical role of ROR t in a new thymic pathway leading to IL-17-producing invariant NKT cell differentiation
title_full Critical role of ROR t in a new thymic pathway leading to IL-17-producing invariant NKT cell differentiation
title_fullStr Critical role of ROR t in a new thymic pathway leading to IL-17-producing invariant NKT cell differentiation
title_full_unstemmed Critical role of ROR t in a new thymic pathway leading to IL-17-producing invariant NKT cell differentiation
title_sort Critical role of ROR t in a new thymic pathway leading to IL-17-producing invariant NKT cell differentiation
author Michel, Marie-Laure
author_facet Michel, Marie-Laure
Cruz, Daniella Mendes da
Keller, Alexandre Castro
Lochner, Matthias
Schneider, Elke
Dy, Michel
Ebert, Gérard
Moraes, Maria C. Leite de
author_role author
author2 Cruz, Daniella Mendes da
Keller, Alexandre Castro
Lochner, Matthias
Schneider, Elke
Dy, Michel
Ebert, Gérard
Moraes, Maria C. Leite de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Michel, Marie-Laure
Cruz, Daniella Mendes da
Keller, Alexandre Castro
Lochner, Matthias
Schneider, Elke
Dy, Michel
Ebert, Gérard
Moraes, Maria C. Leite de
dc.subject.other.pt_BR.fl_str_mv Células T Matadoras Naturais
Citocinas
topic Células T Matadoras Naturais
Citocinas
Thymic precursors
-GalCer
CD1d
Cytokines
dc.subject.en.pt_BR.fl_str_mv Thymic precursors
-GalCer
CD1d
Cytokines
description Université Paris Descartes. Faculté de Médecine. Centre National de la Recherche Scientifique. Unité Mixte de Recherche 8147. Hôpital Necker. Paris, France.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2020-02-11T14:08:55Z
dc.date.available.fl_str_mv 2020-02-11T14:08:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MICHEL, Marie-Laure et al. Critical role of ROR t in a new thymic pathway leading to IL-17-producing invariant NKT cell differentiation. PNAS, v; 105. n. 50, p. 19845-19850, Dec. 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/39842
dc.identifier.issn.pt_BR.fl_str_mv 0027-8424
dc.identifier.doi.none.fl_str_mv 10.1111/j.1472-765X.2008.02322.x
dc.identifier.eissn.none.fl_str_mv 1091-6490
identifier_str_mv MICHEL, Marie-Laure et al. Critical role of ROR t in a new thymic pathway leading to IL-17-producing invariant NKT cell differentiation. PNAS, v; 105. n. 50, p. 19845-19850, Dec. 2008.
0027-8424
10.1111/j.1472-765X.2008.02322.x
1091-6490
url https://www.arca.fiocruz.br/handle/icict/39842
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv National Academy of Sciences
publisher.none.fl_str_mv National Academy of Sciences
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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