Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil

Detalhes bibliográficos
Autor(a) principal: Reis, Rosani Santos
Data de Publicação: 2009
Outros Autores: Almeida-Paes, Rodrigo, Muniz, Mauro de Medeiros, Tavares, Patrícia Morais e Silva, Monteiro, Paulo Cezar Fialho, Schubach, Tânia Maria Pacheco, Gutierrez-Galhardo, Maria Clara, Zancopé-Oliveira, Rosely Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/38969
Resumo: Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
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spelling Reis, Rosani SantosAlmeida-Paes, RodrigoMuniz, Mauro de MedeirosTavares, Patrícia Morais e SilvaMonteiro, Paulo Cezar FialhoSchubach, Tânia Maria PachecoGutierrez-Galhardo, Maria ClaraZancopé-Oliveira, Rosely Maria2020-01-03T15:08:54Z2020-01-03T15:08:54Z2009REIS, Rosani Santos et al. Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 5, p. 769-774, Aug. 2009.0074-0276https://www.arca.fiocruz.br/handle/icict/3896910.1590/s0074-027620090005000181678-8060engFiocruz/IOCMolecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.An epidemic of sporotrichosis, a subcutaneous mycosis caused by the fungus Sporothrix schenckii, is ongoing in Rio de Janeiro, Brazil, in which cases of human infection are related to exposure to cats. In an attempt to demonstrate the zoonotic character of this epidemic using molecular methodology, we characterised by DNA-based typing methods 19 human and 25 animal S. schenckii isolates from the epidemic, as well as two control strains. To analyse the isolates, the random amplified polymorphic DNA (RAPD) technique was performed using three different primers, together with DNA fingerprinting using the minisatellite derived from the wild-type phage M13 core-sequence. The analyses generated amplicons with considerable polymorphism. Although isolates exhibited high levels of genetic relatedness, they could be clustered into 5-10 genotypes. The RAPD profiles of epidemic S. schenckii isolates could be distinguished from that of the United States isolate, displaying 20% similarity to each primer and 60% when amplified with the M13 primer. DNA fingerprinting of S. schenckii isolated from the nails (42.8%) and the oral cavities (66%) of cats were identical to related human samples, suggesting that there is a common infection source for animals and humans in this epidemic. It is clear that cats act as a vehicle for dissemination of S. schenckii.SporotrichosisZoonotic transmissionRAPDDNA fingerprintinginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/38969/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Reis_Rosani_etal_INI_2009.pdfve_Reis_Rosani_etal_INI_2009.pdfapplication/pdf700850https://www.arca.fiocruz.br/bitstream/icict/38969/2/ve_Reis_Rosani_etal_INI_2009.pdf6d9bea9e9bb7e58f4e700930b6a1fc3fMD52TEXTve_Reis_Rosani_etal_INI_2009.pdf.txtve_Reis_Rosani_etal_INI_2009.pdf.txtExtracted texttext/plain25717https://www.arca.fiocruz.br/bitstream/icict/38969/3/ve_Reis_Rosani_etal_INI_2009.pdf.txtd2d694506289f336f435ccb3543aa599MD53icict/389692023-10-25 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dc.title.pt_BR.fl_str_mv Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil
title Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil
spellingShingle Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil
Reis, Rosani Santos
Sporotrichosis
Zoonotic transmission
RAPD
DNA fingerprinting
title_short Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil
title_full Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil
title_fullStr Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil
title_full_unstemmed Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil
title_sort Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil
author Reis, Rosani Santos
author_facet Reis, Rosani Santos
Almeida-Paes, Rodrigo
Muniz, Mauro de Medeiros
Tavares, Patrícia Morais e Silva
Monteiro, Paulo Cezar Fialho
Schubach, Tânia Maria Pacheco
Gutierrez-Galhardo, Maria Clara
Zancopé-Oliveira, Rosely Maria
author_role author
author2 Almeida-Paes, Rodrigo
Muniz, Mauro de Medeiros
Tavares, Patrícia Morais e Silva
Monteiro, Paulo Cezar Fialho
Schubach, Tânia Maria Pacheco
Gutierrez-Galhardo, Maria Clara
Zancopé-Oliveira, Rosely Maria
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Reis, Rosani Santos
Almeida-Paes, Rodrigo
Muniz, Mauro de Medeiros
Tavares, Patrícia Morais e Silva
Monteiro, Paulo Cezar Fialho
Schubach, Tânia Maria Pacheco
Gutierrez-Galhardo, Maria Clara
Zancopé-Oliveira, Rosely Maria
dc.subject.en.pt_BR.fl_str_mv Sporotrichosis
Zoonotic transmission
RAPD
DNA fingerprinting
topic Sporotrichosis
Zoonotic transmission
RAPD
DNA fingerprinting
description Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2020-01-03T15:08:54Z
dc.date.available.fl_str_mv 2020-01-03T15:08:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv REIS, Rosani Santos et al. Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 5, p. 769-774, Aug. 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/38969
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/s0074-02762009000500018
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv REIS, Rosani Santos et al. Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 5, p. 769-774, Aug. 2009.
0074-0276
10.1590/s0074-02762009000500018
1678-8060
url https://www.arca.fiocruz.br/handle/icict/38969
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fiocruz/IOC
publisher.none.fl_str_mv Fiocruz/IOC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/38969/2/ve_Reis_Rosani_etal_INI_2009.pdf
https://www.arca.fiocruz.br/bitstream/icict/38969/3/ve_Reis_Rosani_etal_INI_2009.pdf.txt
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