Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/38969 |
Resumo: | Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. |
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Reis, Rosani SantosAlmeida-Paes, RodrigoMuniz, Mauro de MedeirosTavares, Patrícia Morais e SilvaMonteiro, Paulo Cezar FialhoSchubach, Tânia Maria PachecoGutierrez-Galhardo, Maria ClaraZancopé-Oliveira, Rosely Maria2020-01-03T15:08:54Z2020-01-03T15:08:54Z2009REIS, Rosani Santos et al. Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 5, p. 769-774, Aug. 2009.0074-0276https://www.arca.fiocruz.br/handle/icict/3896910.1590/s0074-027620090005000181678-8060engFiocruz/IOCMolecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.An epidemic of sporotrichosis, a subcutaneous mycosis caused by the fungus Sporothrix schenckii, is ongoing in Rio de Janeiro, Brazil, in which cases of human infection are related to exposure to cats. In an attempt to demonstrate the zoonotic character of this epidemic using molecular methodology, we characterised by DNA-based typing methods 19 human and 25 animal S. schenckii isolates from the epidemic, as well as two control strains. To analyse the isolates, the random amplified polymorphic DNA (RAPD) technique was performed using three different primers, together with DNA fingerprinting using the minisatellite derived from the wild-type phage M13 core-sequence. The analyses generated amplicons with considerable polymorphism. Although isolates exhibited high levels of genetic relatedness, they could be clustered into 5-10 genotypes. The RAPD profiles of epidemic S. schenckii isolates could be distinguished from that of the United States isolate, displaying 20% similarity to each primer and 60% when amplified with the M13 primer. DNA fingerprinting of S. schenckii isolated from the nails (42.8%) and the oral cavities (66%) of cats were identical to related human samples, suggesting that there is a common infection source for animals and humans in this epidemic. It is clear that cats act as a vehicle for dissemination of S. schenckii.SporotrichosisZoonotic transmissionRAPDDNA fingerprintinginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/38969/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Reis_Rosani_etal_INI_2009.pdfve_Reis_Rosani_etal_INI_2009.pdfapplication/pdf700850https://www.arca.fiocruz.br/bitstream/icict/38969/2/ve_Reis_Rosani_etal_INI_2009.pdf6d9bea9e9bb7e58f4e700930b6a1fc3fMD52TEXTve_Reis_Rosani_etal_INI_2009.pdf.txtve_Reis_Rosani_etal_INI_2009.pdf.txtExtracted texttext/plain25717https://www.arca.fiocruz.br/bitstream/icict/38969/3/ve_Reis_Rosani_etal_INI_2009.pdf.txtd2d694506289f336f435ccb3543aa599MD53icict/389692023-10-25 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dc.title.pt_BR.fl_str_mv |
Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil |
title |
Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil |
spellingShingle |
Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil Reis, Rosani Santos Sporotrichosis Zoonotic transmission RAPD DNA fingerprinting |
title_short |
Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil |
title_full |
Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil |
title_fullStr |
Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil |
title_full_unstemmed |
Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil |
title_sort |
Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil |
author |
Reis, Rosani Santos |
author_facet |
Reis, Rosani Santos Almeida-Paes, Rodrigo Muniz, Mauro de Medeiros Tavares, Patrícia Morais e Silva Monteiro, Paulo Cezar Fialho Schubach, Tânia Maria Pacheco Gutierrez-Galhardo, Maria Clara Zancopé-Oliveira, Rosely Maria |
author_role |
author |
author2 |
Almeida-Paes, Rodrigo Muniz, Mauro de Medeiros Tavares, Patrícia Morais e Silva Monteiro, Paulo Cezar Fialho Schubach, Tânia Maria Pacheco Gutierrez-Galhardo, Maria Clara Zancopé-Oliveira, Rosely Maria |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Reis, Rosani Santos Almeida-Paes, Rodrigo Muniz, Mauro de Medeiros Tavares, Patrícia Morais e Silva Monteiro, Paulo Cezar Fialho Schubach, Tânia Maria Pacheco Gutierrez-Galhardo, Maria Clara Zancopé-Oliveira, Rosely Maria |
dc.subject.en.pt_BR.fl_str_mv |
Sporotrichosis Zoonotic transmission RAPD DNA fingerprinting |
topic |
Sporotrichosis Zoonotic transmission RAPD DNA fingerprinting |
description |
Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. |
publishDate |
2009 |
dc.date.issued.fl_str_mv |
2009 |
dc.date.accessioned.fl_str_mv |
2020-01-03T15:08:54Z |
dc.date.available.fl_str_mv |
2020-01-03T15:08:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
REIS, Rosani Santos et al. Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 5, p. 769-774, Aug. 2009. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/38969 |
dc.identifier.issn.pt_BR.fl_str_mv |
0074-0276 |
dc.identifier.doi.none.fl_str_mv |
10.1590/s0074-02762009000500018 |
dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
identifier_str_mv |
REIS, Rosani Santos et al. Molecular characterisation of Sporothrix schenckii isolates from humans and cats involved in the sporotrichosis epidemic in Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 104, n. 5, p. 769-774, Aug. 2009. 0074-0276 10.1590/s0074-02762009000500018 1678-8060 |
url |
https://www.arca.fiocruz.br/handle/icict/38969 |
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eng |
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eng |
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Fiocruz/IOC |
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Fiocruz/IOC |
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