Characterization of the T-Cell receptor Vβ repertoire in the human immune response against Leishmania parasites
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2006 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/2754 |
Resumo: | Agradecimentos a Sílvia Cardoso Andrade (falecida) e Jorge Tolentino pela assistência técnica. |
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Clarêncio, JorgeOliveira, Camila Indiani deArruda, Maria da Gloria BomfimPompeu, Margarida Maria de LimaTeixeira, Maria JâniaBessa, Theolis Costa BarbosaSouza Neto, Sebastião MartinsCarvalho Filho, Edgar MarcelinoBrodskyn, Claudia IdaBarral, Aldina Maria PradoBarral Netto, Manoel2011-07-14T18:46:52Z2011-07-14T18:46:52Z2006CLARÊNCIO, J. et al. Characterization of the T-Cell receptor Vβ repertoire in the human immune response against Leishmania parasites. Infection and Immunity, v.74, n.8, p.4757–4765, 2006.0019-9567https://www.arca.fiocruz.br/handle/icict/275410.1128/IAI.00265-06Agradecimentos a Sílvia Cardoso Andrade (falecida) e Jorge Tolentino pela assistência técnica.Este trabalho foi apoiado pelo CNPq e FIOCRUZ.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilUniversidade Federal do Ceará. Núcleo de Medicina Tropical. Fortaleza, CE, BrasilUniversidade Federal do Ceará. Núcleo de Medicina Tropical. Fortaleza, CE, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilInstituto de Investigação em Imunologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Biointeração. Salvador, BA, BrasilInstituto de Investigação em Imunologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilInstituto de Investigação em Imunologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilInstituto de Investigação em Imunologia. São Paulo, SP, BrasilIn order to explore a possible presence of hyperreactive T-cell clones in human cutaneous leishmaniasis (CL), we have investigated, by flow cytometry, the expression of V chains of T-cell receptors (TCRs) in the following types of cells: (i) peripheral blood mononuclear cells (PBMCs) from CL patients, which were then compared to those from normal volunteers; (ii) unstimulated and soluble Leishmania antigen-stimulated draining lymph node cells from CL patients; (iii) PBMCs from volunteers before versus after Leishmania immunization; and (iv) PBMCs from healthy volunteers that were primed in vitro with live Leishmania parasites. Our results show a modulation in the TCR V repertoire during CL and after antigen stimulation of patients’ cells. Vaccination, however, leads to a broad expansion of different V TCRs. We also observed an association between TCR V 12 expression, T-cell activation, and gamma interferon production upon in vitro priming with Leishmania. Collectively, these results both indicate that infection with live parasites or exposure to parasite antigen can modulate the TCR V repertoire and suggest that TCR V 12 may be implicated in the response to Leishmania.engTCRResposta Imune HumanaParasitas LeishmaniaTCRHuman Immune ResponseLeishmania ParasitesGenes Codificadores da Cadeia beta de Receptores de Linfócitos TLeishmaniaCharacterization of the T-Cell receptor Vβ repertoire in the human immune response against Leishmania parasitesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALClarencio_Oliveira_Bomfim_etal.pdfClarencio_Oliveira_Bomfim_etal.pdfapplication/pdf370997https://www.arca.fiocruz.br/bitstream/icict/2754/1/Clarencio_Oliveira_Bomfim_etal.pdf61d4e0d3ffcc232086813c641e365f18MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/2754/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTClarencio_Oliveira_Bomfim_etal.pdf.txtClarencio_Oliveira_Bomfim_etal.pdf.txtExtracted texttext/plain46936https://www.arca.fiocruz.br/bitstream/icict/2754/5/Clarencio_Oliveira_Bomfim_etal.pdf.txtab7e093427c03223fc353a554ba7409eMD55THUMBNAILClarencio_Oliveira_Bomfim_etal.pdf.jpgClarencio_Oliveira_Bomfim_etal.pdf.jpgGenerated Thumbnailimage/jpeg2387https://www.arca.fiocruz.br/bitstream/icict/2754/4/Clarencio_Oliveira_Bomfim_etal.pdf.jpgead806af60f6210df0ce927ef5c63056MD54icict/27542023-03-15 14:33:18.18oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Characterization of the T-Cell receptor Vβ repertoire in the human immune response against Leishmania parasites |
| title |
Characterization of the T-Cell receptor Vβ repertoire in the human immune response against Leishmania parasites |
| spellingShingle |
Characterization of the T-Cell receptor Vβ repertoire in the human immune response against Leishmania parasites Clarêncio, Jorge TCR Resposta Imune Humana Parasitas Leishmania TCR Human Immune Response Leishmania Parasites Genes Codificadores da Cadeia beta de Receptores de Linfócitos T Leishmania |
| title_short |
Characterization of the T-Cell receptor Vβ repertoire in the human immune response against Leishmania parasites |
| title_full |
Characterization of the T-Cell receptor Vβ repertoire in the human immune response against Leishmania parasites |
| title_fullStr |
Characterization of the T-Cell receptor Vβ repertoire in the human immune response against Leishmania parasites |
| title_full_unstemmed |
Characterization of the T-Cell receptor Vβ repertoire in the human immune response against Leishmania parasites |
| title_sort |
Characterization of the T-Cell receptor Vβ repertoire in the human immune response against Leishmania parasites |
| author |
Clarêncio, Jorge |
| author_facet |
Clarêncio, Jorge Oliveira, Camila Indiani de Arruda, Maria da Gloria Bomfim Pompeu, Margarida Maria de Lima Teixeira, Maria Jânia Bessa, Theolis Costa Barbosa Souza Neto, Sebastião Martins Carvalho Filho, Edgar Marcelino Brodskyn, Claudia Ida Barral, Aldina Maria Prado Barral Netto, Manoel |
| author_role |
author |
| author2 |
Oliveira, Camila Indiani de Arruda, Maria da Gloria Bomfim Pompeu, Margarida Maria de Lima Teixeira, Maria Jânia Bessa, Theolis Costa Barbosa Souza Neto, Sebastião Martins Carvalho Filho, Edgar Marcelino Brodskyn, Claudia Ida Barral, Aldina Maria Prado Barral Netto, Manoel |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Clarêncio, Jorge Oliveira, Camila Indiani de Arruda, Maria da Gloria Bomfim Pompeu, Margarida Maria de Lima Teixeira, Maria Jânia Bessa, Theolis Costa Barbosa Souza Neto, Sebastião Martins Carvalho Filho, Edgar Marcelino Brodskyn, Claudia Ida Barral, Aldina Maria Prado Barral Netto, Manoel |
| dc.subject.other.pt_BR.fl_str_mv |
TCR Resposta Imune Humana Parasitas Leishmania |
| topic |
TCR Resposta Imune Humana Parasitas Leishmania TCR Human Immune Response Leishmania Parasites Genes Codificadores da Cadeia beta de Receptores de Linfócitos T Leishmania |
| dc.subject.en.pt_BR.fl_str_mv |
TCR Human Immune Response Leishmania Parasites |
| dc.subject.decs.pt_BR.fl_str_mv |
Genes Codificadores da Cadeia beta de Receptores de Linfócitos T Leishmania |
| description |
Agradecimentos a Sílvia Cardoso Andrade (falecida) e Jorge Tolentino pela assistência técnica. |
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2006 |
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2006 |
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2011-07-14T18:46:52Z |
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2011-07-14T18:46:52Z |
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info:eu-repo/semantics/article |
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CLARÊNCIO, J. et al. Characterization of the T-Cell receptor Vβ repertoire in the human immune response against Leishmania parasites. Infection and Immunity, v.74, n.8, p.4757–4765, 2006. |
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0019-9567 |
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10.1128/IAI.00265-06 |
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CLARÊNCIO, J. et al. Characterization of the T-Cell receptor Vβ repertoire in the human immune response against Leishmania parasites. Infection and Immunity, v.74, n.8, p.4757–4765, 2006. 0019-9567 10.1128/IAI.00265-06 |
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