Secretome analysis performed during in vitro cardiac differentiation: Discovering the cardiac microenvironment

Detalhes bibliográficos
Autor(a) principal: Robert, Anny Waloski
Data de Publicação: 2020
Outros Autores: Pereira, Isabela Tiemy, Dallagiovanna, Bruno, Stimamiglio, Marco Augusto
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/40813
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
id CRUZ_7396b6d0d3ba17150c8cc02127e66632
oai_identifier_str oai:www.arca.fiocruz.br:icict/40813
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Robert, Anny WaloskiPereira, Isabela TiemyDallagiovanna, BrunoStimamiglio, Marco Augusto2020-04-16T19:39:38Z2020-04-16T19:39:38Z2020ROBERT, Anny Waloski et al. Secretome analysis performed during in vitro cardiac differentiation: Discovering the cardiac microenvironment. Frontiers in Cell and Developmental Biology, v. 8, n. 49, p. 1-15, 2020.2296-634Xhttps://www.arca.fiocruz.br/handle/icict/4081310.3389/fcell.2020.00049porFrontiers MediaSecretome analysis performed during in vitro cardiac differentiation: Discovering the cardiac microenvironmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Human pluripotent stem cells are an important tool for the study of developmental processes, such as cardiomyogenic differentiation. Despite the advances made in this field, the molecular and cellular signals involved in the commitment of embryonic stem cells to the cardiac phenotype are still under investigation. Therefore, this study focuses on identifying the extracellular signals involved in in vitro cardiac differentiation of human embryonic stem cells. Using a three-dimensional cardiomyogenic differentiation protocol, the conditioned medium and the extracellular matrix (ECM) of embryoid body cultures were collected and characterized at four specific time points. Mass spectrometry (MS) and antibody array analysis of the secretome identified a number of secreted proteins related to signaling pathways, such as Wnt and TGFβ, as well as many ECM proteins. When comparing the proteins identified at selected time points, our data pointed out protein interactions and biological process related to cardiac differentiation. Interestingly, the great changes in secretome profile occurred during the cardiac progenitor specification. The secretome results were also compared with our previous RNAseq data, indicating that the secreted proteins undergo some level of gene regulation. During cardiac commitment it was observed an increase in complexity of the ECM, and some proteins as IGFBP7, FN1, HSPG2, as well as other members of the basal lamina could be highlighted. Thus, these findings contribute valuable information about essential microenvironmental signals working on cardiomyogenic differentiation that may be used in future strategies for cardiac differentiation, cardiomyocyte maturation, and in advances for future acellular therapies.Extracellular MatrixPluripotent Stem CellsCell DifferentiationMyocytes, CardiacEmbryonic Stem CellsCulture Media, ConditionedCélulas Madre PluripotentesDiferenciación CelularMiocitos CardíacosCélulas Madre EmbrionariasMatriz ExtracelularCélulas-Tronco PluripotentesDiferenciação CelularMiócitos CardíacosCélulas-Tronco EmbrionáriasMeios de Cultivo Condicionadosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/40813/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALfcell-08-0004.pdffcell-08-0004.pdfapplication/pdf18420620https://www.arca.fiocruz.br/bitstream/icict/40813/2/fcell-08-0004.pdfc16eecdac18ef4e1028f5de73d5926a5MD52TEXTfcell-08-0004.pdf.txtfcell-08-0004.pdf.txtExtracted texttext/plain80720https://www.arca.fiocruz.br/bitstream/icict/40813/3/fcell-08-0004.pdf.txta7a8c5ae4f1e24c6bbea2cecd53cca1bMD53icict/408132020-04-17 02:09:35.229oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352020-04-17T05:09:35Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Secretome analysis performed during in vitro cardiac differentiation: Discovering the cardiac microenvironment
title Secretome analysis performed during in vitro cardiac differentiation: Discovering the cardiac microenvironment
spellingShingle Secretome analysis performed during in vitro cardiac differentiation: Discovering the cardiac microenvironment
Robert, Anny Waloski
Extracellular Matrix
Pluripotent Stem Cells
Cell Differentiation
Myocytes, Cardiac
Embryonic Stem Cells
Culture Media, Conditioned
Células Madre Pluripotentes
Diferenciación Celular
Miocitos Cardíacos
Células Madre Embrionarias
Matriz Extracelular
Células-Tronco Pluripotentes
Diferenciação Celular
Miócitos Cardíacos
Células-Tronco Embrionárias
Meios de Cultivo Condicionados
title_short Secretome analysis performed during in vitro cardiac differentiation: Discovering the cardiac microenvironment
title_full Secretome analysis performed during in vitro cardiac differentiation: Discovering the cardiac microenvironment
title_fullStr Secretome analysis performed during in vitro cardiac differentiation: Discovering the cardiac microenvironment
title_full_unstemmed Secretome analysis performed during in vitro cardiac differentiation: Discovering the cardiac microenvironment
title_sort Secretome analysis performed during in vitro cardiac differentiation: Discovering the cardiac microenvironment
author Robert, Anny Waloski
author_facet Robert, Anny Waloski
Pereira, Isabela Tiemy
Dallagiovanna, Bruno
Stimamiglio, Marco Augusto
author_role author
author2 Pereira, Isabela Tiemy
Dallagiovanna, Bruno
Stimamiglio, Marco Augusto
author2_role author
author
author
dc.contributor.author.fl_str_mv Robert, Anny Waloski
Pereira, Isabela Tiemy
Dallagiovanna, Bruno
Stimamiglio, Marco Augusto
dc.subject.en.pt_BR.fl_str_mv Extracellular Matrix
Pluripotent Stem Cells
Cell Differentiation
Myocytes, Cardiac
Embryonic Stem Cells
Culture Media, Conditioned
topic Extracellular Matrix
Pluripotent Stem Cells
Cell Differentiation
Myocytes, Cardiac
Embryonic Stem Cells
Culture Media, Conditioned
Células Madre Pluripotentes
Diferenciación Celular
Miocitos Cardíacos
Células Madre Embrionarias
Matriz Extracelular
Células-Tronco Pluripotentes
Diferenciação Celular
Miócitos Cardíacos
Células-Tronco Embrionárias
Meios de Cultivo Condicionados
dc.subject.es.pt_BR.fl_str_mv Células Madre Pluripotentes
Diferenciación Celular
Miocitos Cardíacos
Células Madre Embrionarias
dc.subject.decs.pt_BR.fl_str_mv Matriz Extracelular
Células-Tronco Pluripotentes
Diferenciação Celular
Miócitos Cardíacos
Células-Tronco Embrionárias
Meios de Cultivo Condicionados
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-04-16T19:39:38Z
dc.date.available.fl_str_mv 2020-04-16T19:39:38Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ROBERT, Anny Waloski et al. Secretome analysis performed during in vitro cardiac differentiation: Discovering the cardiac microenvironment. Frontiers in Cell and Developmental Biology, v. 8, n. 49, p. 1-15, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/40813
dc.identifier.issn.pt_BR.fl_str_mv 2296-634X
dc.identifier.doi.none.fl_str_mv 10.3389/fcell.2020.00049
identifier_str_mv ROBERT, Anny Waloski et al. Secretome analysis performed during in vitro cardiac differentiation: Discovering the cardiac microenvironment. Frontiers in Cell and Developmental Biology, v. 8, n. 49, p. 1-15, 2020.
2296-634X
10.3389/fcell.2020.00049
url https://www.arca.fiocruz.br/handle/icict/40813
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/40813/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/40813/2/fcell-08-0004.pdf
https://www.arca.fiocruz.br/bitstream/icict/40813/3/fcell-08-0004.pdf.txt
bitstream.checksum.fl_str_mv 783568c2893d2e25a99990b126be1772
c16eecdac18ef4e1028f5de73d5926a5
a7a8c5ae4f1e24c6bbea2cecd53cca1b
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813009250883993600

Registros relacionados