Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy

Detalhes bibliográficos
Autor(a) principal: Marçal, Pedro Henrique Ferreira
Data de Publicação: 2018
Outros Autores: Fraga, Lúcia Alves de Oliveira, Mattos, Ana Márcia Menezes de, Menegati, Laura, Coelho, Angélica da Conceição Oliveira, Pinheiro, Roberta Olmo, Sarno, Euzenir Nunes, Duthie, Malcolm S., Teixeira, Henrique Couto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/26229
Resumo: Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil.
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spelling Marçal, Pedro Henrique FerreiraFraga, Lúcia Alves de OliveiraMattos, Ana Márcia Menezes deMenegati, LauraCoelho, Angélica da Conceição OliveiraPinheiro, Roberta OlmoSarno, Euzenir NunesDuthie, Malcolm S.Teixeira, Henrique Couto2018-05-03T13:02:20Z2018-05-03T13:02:20Z2018MARÇAL, Pedro Henrique Ferreira; et al. Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy. Mem Inst Oswaldo Cruz, v. 113, n.5, e170467, 2018.0074-0276https://www.arca.fiocruz.br/handle/icict/2622910.1590/0074-027601704671678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzHanseníasesorodiagnósticoAntígeno do M. lepraeIsotipos de ImunoglobulinasLeprosyserodiagnosisM. leprae antigenLID-1natural octyl disaccharideIgG1 immunoglobulin subclassUtility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Instituto de Ciências da Vida. Governador Valadares, MG, Brasil.Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil.Universidade Federal de Juiz de Fora. Faculdade de Enfermagem. Departamento de Enfermagem Básica. Juiz de Fora, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Infectious Disease Research Institute. Seattle, WA, USA.Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil.Leprosy remains a health problem in many countries, with difficulties in diagnosis resulting in delayed treatment and more severe disabilities. Antibodies against several Mycobacterium leprae antigens have, however, shown value as diagnostic and/or prognostic markers. OBJECTIVES The objective of this study was to evaluate serum immunoglobulin (Ig) IgM and IgG subclass reactivity against three M. leprae specific antigens: NDO-HSA, a conjugate formed by natural octyl disaccharide bound to human serum albumin; LID-1, the fusion protein product of the ml0405 and ml2331 genes; and NDO-LID, a combination of LID-1 and NDO. METHODS Sera from healthy controls, paucibacillary (PB) and multibacillary (MB) leprosy patients, and their respective household contacts, were evaluated for the presence of antigen-specific IgM, IgG, and IgG subclass antibodies by enzyme-linked immunosorbent assay (ELISA). The sensitivity and specificity of each ELISA were evaluated by receiver operating characteristic (ROC) curve analysis. FINDINGS Our data confirm that serum IgM antibodies against NDO-HSA and IgG antibodies against LID-1, as well as IgG/M antibodies against NDO-LID, are markedly increased in MB patients. For the first time, our data reveal a selective increase in IgG1 and IgG3 antibodies against LID-1 and NDO-LID in MB patients, demonstrating that these antibody isotypes are suitable for differentiation between MB and PB patients. ROC curve analysis indicates an improved capacity for diagnosing MB leprosy patients using the detection of IgG antibodies, particularly the IgG1 isotype, specific to LID-1 and NDO-LID over the performance levels attained with NDO-HSA. CONCLUSIONS Our findings indicate that serological tests based on the detection of antigen-specific IgG1 antibodies are a useful tool to differentiate MB from PB patients, and indicate the enhanced performance of the LID-1 and NDO-LID antigens in the serodiagnosis of leprosy.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/26229/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALrobertaO_pinheiro_etal_IOC_2018.pdfrobertaO_pinheiro_etal_IOC_2018.pdfapplication/pdf742365https://www.arca.fiocruz.br/bitstream/icict/26229/2/robertaO_pinheiro_etal_IOC_2018.pdff31dc862c218949907f11827ebecdb9dMD52TEXTrobertaO_pinheiro_etal_IOC_2018.pdf.txtrobertaO_pinheiro_etal_IOC_2018.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy
title Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy
spellingShingle Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy
Marçal, Pedro Henrique Ferreira
Hanseníase
sorodiagnóstico
Antígeno do M. leprae
Isotipos de Imunoglobulinas
Leprosy
serodiagnosis
M. leprae antigen
LID-1
natural octyl disaccharide
IgG1 immunoglobulin subclass
title_short Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy
title_full Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy
title_fullStr Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy
title_full_unstemmed Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy
title_sort Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy
author Marçal, Pedro Henrique Ferreira
author_facet Marçal, Pedro Henrique Ferreira
Fraga, Lúcia Alves de Oliveira
Mattos, Ana Márcia Menezes de
Menegati, Laura
Coelho, Angélica da Conceição Oliveira
Pinheiro, Roberta Olmo
Sarno, Euzenir Nunes
Duthie, Malcolm S.
Teixeira, Henrique Couto
author_role author
author2 Fraga, Lúcia Alves de Oliveira
Mattos, Ana Márcia Menezes de
Menegati, Laura
Coelho, Angélica da Conceição Oliveira
Pinheiro, Roberta Olmo
Sarno, Euzenir Nunes
Duthie, Malcolm S.
Teixeira, Henrique Couto
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Marçal, Pedro Henrique Ferreira
Fraga, Lúcia Alves de Oliveira
Mattos, Ana Márcia Menezes de
Menegati, Laura
Coelho, Angélica da Conceição Oliveira
Pinheiro, Roberta Olmo
Sarno, Euzenir Nunes
Duthie, Malcolm S.
Teixeira, Henrique Couto
dc.subject.other.pt_BR.fl_str_mv Hanseníase
sorodiagnóstico
Antígeno do M. leprae
Isotipos de Imunoglobulinas
topic Hanseníase
sorodiagnóstico
Antígeno do M. leprae
Isotipos de Imunoglobulinas
Leprosy
serodiagnosis
M. leprae antigen
LID-1
natural octyl disaccharide
IgG1 immunoglobulin subclass
dc.subject.en.pt_BR.fl_str_mv Leprosy
serodiagnosis
M. leprae antigen
LID-1
natural octyl disaccharide
IgG1 immunoglobulin subclass
description Universidade Federal de Juiz de Fora. Instituto de Ciências Biológicas. Departamento de Parasitologia, Microbiologia e Imunologia. Juiz de Fora, MG, Brasil.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-05-03T13:02:20Z
dc.date.available.fl_str_mv 2018-05-03T13:02:20Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARÇAL, Pedro Henrique Ferreira; et al. Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy. Mem Inst Oswaldo Cruz, v. 113, n.5, e170467, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/26229
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760170467
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv MARÇAL, Pedro Henrique Ferreira; et al. Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy. Mem Inst Oswaldo Cruz, v. 113, n.5, e170467, 2018.
0074-0276
10.1590/0074-02760170467
1678-8060
url https://www.arca.fiocruz.br/handle/icict/26229
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/26229/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/26229/2/robertaO_pinheiro_etal_IOC_2018.pdf
https://www.arca.fiocruz.br/bitstream/icict/26229/3/robertaO_pinheiro_etal_IOC_2018.pdf.txt
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