Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFJF |
| Texto Completo: | http://dx.doi.org/10.1590/0074-02760170467 https://repositorio.ufjf.br/jspui/handle/ufjf/9248 |
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2019-02-27T15:14:37Z2019-02-252019-02-27T15:14:37Z2018-021135918http://dx.doi.org/10.1590/0074-02760170467https://repositorio.ufjf.br/jspui/handle/ufjf/9248-BACKGROUND Leprosy remains a health problem in many countries, with difficulties in diagnosis resulting in delayed treatment and more severe disabilities. Antibodies against several Mycobacterium leprae antigens have, however, shown value as diagnostic and/or prognostic markers. OBJECTIVES The objective of this study was to evaluate serum immunoglobulin (Ig) IgM and IgG subclass reactivity against three M. leprae specific antigens: NDO-HSA, a conjugate formed by natural octyl disaccharide bound to human serum albumin; LID-1, the fusion protein product of the ml0405 and ml2331 genes; and NDO-LID, a combination of LID-1 and NDO. METHODS Sera from healthy controls, paucibacillary (PB) and multibacillary (MB) leprosy patients, and their respective household contacts, were evaluated for the presence of antigen-specific IgM, IgG, and IgG subclass antibodies by enzyme-linked immunosorbent assay (ELISA). The sensitivity and specificity of each ELISA were evaluated by receiver operating characteristic (ROC) curve analysis. FINDINGS Our data confirm that serum IgM antibodies against NDO-HSA and IgG antibodies against LID-1, as well as IgG/M antibodies against NDO-LID, are markedly increased in MB patients. For the first time, our data reveal a selective increase in IgG1 and IgG3 antibodies against LID-1 and NDO-LID in MB patients, demonstrating that these antibody isotypes are suitable for differentiation between MB and PB patients. ROC curve analysis indicates an improved capacity for diagnosing MB leprosy patients using the detection of IgG antibodies, particularly the IgG1 isotype, specific to LID-1 and NDO-LID over the performance levels attained with NDO-HSA. CONCLUSIONS Our findings indicate that serological tests based on the detection of antigen-specific IgG1 antibodies are a useful tool to differentiate MB from PB patients, and indicate the enhanced performance of the LID-1 and NDO-LID antigens in the serodiagnosis of leprosyeng--BrasilMemórias do Instituto Oswaldo Cruz-LeprosySerodiagnosisM. leprae antigenLID-1Natural octyl disaccharideIgG1 immunoglobulin subclassUtility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleMarçal, Pedro Henrique FerreiraFraga, Lúcia Alves de OliveiraMattos, Ana Márcia Menezes deMenegati, LauraCoelho, Angélica da Conceição OliveiraPinheiro, Roberta OlmoSarno, Euzenir NunesDuthie, Malcolm STeixeira, Henrique Coutoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFJFinstname:Universidade Federal de Juiz de Fora (UFJF)instacron:UFJFTHUMBNAILUtility of immunoglobulin isotypes against LID-1 and NDO-LID for.pdf.jpgUtility of immunoglobulin isotypes against LID-1 and NDO-LID for.pdf.jpgGenerated Thumbnailimage/jpeg1691https://repositorio.ufjf.br/jspui/bitstream/ufjf/9248/4/Utility%20of%20immunoglobulin%20isotypes%20against%20LID-1%20and%20NDO-LID%20for.pdf.jpg75cf18bd4c057383218c83594c79c6deMD54ORIGINALUtility of immunoglobulin isotypes against LID-1 and NDO-LID for.pdfUtility of immunoglobulin isotypes against LID-1 and NDO-LID for.pdfapplication/pdf742365https://repositorio.ufjf.br/jspui/bitstream/ufjf/9248/1/Utility%20of%20immunoglobulin%20isotypes%20against%20LID-1%20and%20NDO-LID%20for.pdff31dc862c218949907f11827ebecdb9dMD51LICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy |
| title |
Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy |
| spellingShingle |
Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy Marçal, Pedro Henrique Ferreira - Leprosy Serodiagnosis M. leprae antigen LID-1 Natural octyl disaccharide IgG1 immunoglobulin subclass |
| title_short |
Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy |
| title_full |
Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy |
| title_fullStr |
Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy |
| title_full_unstemmed |
Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy |
| title_sort |
Utility of immunoglobulin isotypes against LID-1 and NDO-LID for, particularly IgG1, confirming the diagnosis of multibacillary leprosy |
| author |
Marçal, Pedro Henrique Ferreira |
| author_facet |
Marçal, Pedro Henrique Ferreira Fraga, Lúcia Alves de Oliveira Mattos, Ana Márcia Menezes de Menegati, Laura Coelho, Angélica da Conceição Oliveira Pinheiro, Roberta Olmo Sarno, Euzenir Nunes Duthie, Malcolm S Teixeira, Henrique Couto |
| author_role |
author |
| author2 |
Fraga, Lúcia Alves de Oliveira Mattos, Ana Márcia Menezes de Menegati, Laura Coelho, Angélica da Conceição Oliveira Pinheiro, Roberta Olmo Sarno, Euzenir Nunes Duthie, Malcolm S Teixeira, Henrique Couto |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Marçal, Pedro Henrique Ferreira Fraga, Lúcia Alves de Oliveira Mattos, Ana Márcia Menezes de Menegati, Laura Coelho, Angélica da Conceição Oliveira Pinheiro, Roberta Olmo Sarno, Euzenir Nunes Duthie, Malcolm S Teixeira, Henrique Couto |
| dc.subject.cnpq.fl_str_mv |
- |
| topic |
- Leprosy Serodiagnosis M. leprae antigen LID-1 Natural octyl disaccharide IgG1 immunoglobulin subclass |
| dc.subject.por.fl_str_mv |
Leprosy Serodiagnosis M. leprae antigen LID-1 Natural octyl disaccharide IgG1 immunoglobulin subclass |
| description |
- |
| publishDate |
2018 |
| dc.date.issued.fl_str_mv |
2018-02 |
| dc.date.accessioned.fl_str_mv |
2019-02-27T15:14:37Z |
| dc.date.available.fl_str_mv |
2019-02-25 2019-02-27T15:14:37Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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https://repositorio.ufjf.br/jspui/handle/ufjf/9248 |
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http://dx.doi.org/10.1590/0074-02760170467 |
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http://dx.doi.org/10.1590/0074-02760170467 https://repositorio.ufjf.br/jspui/handle/ufjf/9248 |
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eng |
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eng |
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Memórias do Instituto Oswaldo Cruz |
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info:eu-repo/semantics/openAccess |
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openAccess |
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- |
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Brasil |
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reponame:Repositório Institucional da UFJF instname:Universidade Federal de Juiz de Fora (UFJF) instacron:UFJF |
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