DC-SIGN Mediates the Interaction Between Neutrophils and Leishmania amazonensis-Infected Dendritic Cells to Promote DC Maturation and Parasite Elimination

Detalhes bibliográficos
Autor(a) principal: Tiburcio, Rafael
Data de Publicação: 2021
Outros Autores: Melo, Léon Dimitri, Nunes, Sara, Barbosa, Ana Luísa Augusto, Oliveira, Elaine Carvalho de, Suarez, Martha, Borges, Valéria M., Tavares, Natalia, Brodskyn, Claudia Ida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50420
Resumo: National Council of Research (CNPq–Universal) grants 476926/2011-4 for CB and FAPESB APP0108/2016 for VMB. VMB and CB are senior investigators from CNPq. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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spelling Tiburcio, RafaelMelo, Léon DimitriNunes, SaraBarbosa, Ana Luísa AugustoOliveira, Elaine Carvalho deSuarez, MarthaBorges, Valéria M.Tavares, NataliaBrodskyn, Claudia Ida2021-12-14T17:56:45Z2021-12-14T17:56:45Z2021TIBURCIO, Rafael et al. DC-SIGN Mediates the Interaction Between Neutrophils and Leishmania amazonensis-Infected Dendritic Cells to Promote DC Maturation and Parasite Elimination. Frontiers in Immunology, 2021.1664-3224https://www.arca.fiocruz.br/handle/icict/50420National Council of Research (CNPq–Universal) grants 476926/2011-4 for CB and FAPESB APP0108/2016 for VMB. VMB and CB are senior investigators from CNPq. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Instituto de Nacional de Ciência e Tecnologia. Instituto de Investigação em Imunologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Instituto de Nacional de Ciência e Tecnologia. Instituto de Investigação em Imunologia. São Paulo, SP, Brasil.Background: Leishmaniasis is a neglected arthropod-borne disease that affects millions of people worldwide. Successful Leishmania infections require the mitigation of immune cell functions leading to parasite survival and proliferation. A large body of evidence highlights the involvement of neutrophils (PMNs) and dendritic cells (DCs) in the establishment of immunological responses against these parasites. However, few studies, contemplate to what extent these cells interact synergistically to constrain Leishmania infection. Objective: We sought to investigate how PMNs and infected DCs interact in an in vitro model of Leishmania amazonensis infection. Material and Methods: Briefly, human PMNs and DCs were purified from the peripheral blood of healthy donors. Next, PMNs were activated with fibronectin and subsequently co-cultured with L. amazonensis-infected DCs. Results: We observed that L. amazonensis-infected DC exhibited lower rates of infection when co-cultivated with either resting or activated PMNs. Surprisingly, we found that the release of neutrophil enzymes was not involved in Leishmania killing. Next, we showed that the interaction between PMNs and infected-DCs was intermediated by DC-SIGN, further suggesting that parasite elimination occurs in a contact-dependent manner. Furthermore, we also observed that TNFa and ROS production was dependent on DC-SIGN-mediated contact, as well as parasite elimination is dependent on TNFa production in the co-culture. Finally, we observed that direct contact between PMNs and DCs are required to restore the expression of DC maturation molecules during L. amazonensis infection. Conclusion: Our findings suggest that the engagement of direct contact between PMNs and L. amazonensis-infected DC via DC-SIGN is required for the production of inflammatory mediators with subsequent parasite elimination and DC maturation.engFrontiers MediaLeishmanioseNeutrófilosCélulas dendríticasMonócitosMaturaçãoLeishmaniasisPolymorphonuclear cellsMonocyte-derived dendritic cellsDC-SIGNDC maturation markersDC-SIGN Mediates the Interaction Between Neutrophils and Leishmania amazonensis-Infected Dendritic Cells to Promote DC Maturation and Parasite Eliminationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/50420/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALTiburcio, Rafael DC-SIGN... (1).pdfTiburcio, Rafael DC-SIGN... 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dc.title.pt_BR.fl_str_mv DC-SIGN Mediates the Interaction Between Neutrophils and Leishmania amazonensis-Infected Dendritic Cells to Promote DC Maturation and Parasite Elimination
title DC-SIGN Mediates the Interaction Between Neutrophils and Leishmania amazonensis-Infected Dendritic Cells to Promote DC Maturation and Parasite Elimination
spellingShingle DC-SIGN Mediates the Interaction Between Neutrophils and Leishmania amazonensis-Infected Dendritic Cells to Promote DC Maturation and Parasite Elimination
Tiburcio, Rafael
Leishmaniose
Neutrófilos
Células dendríticas
Monócitos
Maturação
Leishmaniasis
Polymorphonuclear cells
Monocyte-derived dendritic cells
DC-SIGN
DC maturation markers
title_short DC-SIGN Mediates the Interaction Between Neutrophils and Leishmania amazonensis-Infected Dendritic Cells to Promote DC Maturation and Parasite Elimination
title_full DC-SIGN Mediates the Interaction Between Neutrophils and Leishmania amazonensis-Infected Dendritic Cells to Promote DC Maturation and Parasite Elimination
title_fullStr DC-SIGN Mediates the Interaction Between Neutrophils and Leishmania amazonensis-Infected Dendritic Cells to Promote DC Maturation and Parasite Elimination
title_full_unstemmed DC-SIGN Mediates the Interaction Between Neutrophils and Leishmania amazonensis-Infected Dendritic Cells to Promote DC Maturation and Parasite Elimination
title_sort DC-SIGN Mediates the Interaction Between Neutrophils and Leishmania amazonensis-Infected Dendritic Cells to Promote DC Maturation and Parasite Elimination
author Tiburcio, Rafael
author_facet Tiburcio, Rafael
Melo, Léon Dimitri
Nunes, Sara
Barbosa, Ana Luísa Augusto
Oliveira, Elaine Carvalho de
Suarez, Martha
Borges, Valéria M.
Tavares, Natalia
Brodskyn, Claudia Ida
author_role author
author2 Melo, Léon Dimitri
Nunes, Sara
Barbosa, Ana Luísa Augusto
Oliveira, Elaine Carvalho de
Suarez, Martha
Borges, Valéria M.
Tavares, Natalia
Brodskyn, Claudia Ida
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Tiburcio, Rafael
Melo, Léon Dimitri
Nunes, Sara
Barbosa, Ana Luísa Augusto
Oliveira, Elaine Carvalho de
Suarez, Martha
Borges, Valéria M.
Tavares, Natalia
Brodskyn, Claudia Ida
dc.subject.other.pt_BR.fl_str_mv Leishmaniose
Neutrófilos
Células dendríticas
Monócitos
Maturação
topic Leishmaniose
Neutrófilos
Células dendríticas
Monócitos
Maturação
Leishmaniasis
Polymorphonuclear cells
Monocyte-derived dendritic cells
DC-SIGN
DC maturation markers
dc.subject.en.pt_BR.fl_str_mv Leishmaniasis
Polymorphonuclear cells
Monocyte-derived dendritic cells
DC-SIGN
DC maturation markers
description National Council of Research (CNPq–Universal) grants 476926/2011-4 for CB and FAPESB APP0108/2016 for VMB. VMB and CB are senior investigators from CNPq. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-12-14T17:56:45Z
dc.date.available.fl_str_mv 2021-12-14T17:56:45Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TIBURCIO, Rafael et al. DC-SIGN Mediates the Interaction Between Neutrophils and Leishmania amazonensis-Infected Dendritic Cells to Promote DC Maturation and Parasite Elimination. Frontiers in Immunology, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50420
dc.identifier.issn.pt_BR.fl_str_mv 1664-3224
identifier_str_mv TIBURCIO, Rafael et al. DC-SIGN Mediates the Interaction Between Neutrophils and Leishmania amazonensis-Infected Dendritic Cells to Promote DC Maturation and Parasite Elimination. Frontiers in Immunology, 2021.
1664-3224
url https://www.arca.fiocruz.br/handle/icict/50420
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50420/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50420/2/Tiburcio%2c%20Rafael%20DC-SIGN...%20%281%29.pdf
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repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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