In vivo imaging of mice infected with bioluminescent Trypanosoma cruzi unveils novel sites of infection
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23948 |
Resumo: | Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Biomagens. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. CENABIO. Núcleo de Biologia Estrutural e Bioimagens. Rio de Janeiro, RJ,, Brasil / Fundação Oswaldo Cruz. Campo Grande, MS, Brasil. |
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Henriques, CristinaPons, Andréa HenriquesBatista, Marcelo MeuserRibeiro, Aline SalgadoSouza, Wanderley de2018-01-18T14:08:56Z2018-01-18T14:08:56Z2014HENRIQUES, Cristina; et al. In vivo imaging of mice infected with bioluminescent Trypanosoma cruzi unveils novel sites of infection. Parasites & Vectors, v.7:89, 15p, 2014.1756-3305https://www.arca.fiocruz.br/handle/icict/2394810.1186/1756-3305-7-89engBioMed CentralTrypanosoma cruziDoença de ChagasLuciferasesBioluminiscenciaTestículoRetoCoraçãoCamundongosTrypanosoma cruziChagas DiseaseLuciferaseBioluminescenceTesticlesRectumHeartMiceIn vivo imaging of mice infected with bioluminescent Trypanosoma cruzi unveils novel sites of infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Biomagens. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. CENABIO. Núcleo de Biologia Estrutural e Bioimagens. Rio de Janeiro, RJ,, Brasil / Fundação Oswaldo Cruz. Campo Grande, MS, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ. Brasi / Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Departamento de Anatomia Patológica e Citopatologia. Rio de Janeiro, RJ, Brasil..Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro-CENABIO. Núcleo de Biologia Estrutural e Biomagens. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Metrologia, Qualidade e Tecnologia. Rio de Janeiro, RJ, Brasil.Background: The development of techniques that allow the imaging of animals infected with parasites expressing luciferase opens up new possibilities for following the fate of parasites in infected mammals. Methods: D-luciferin potassium salt stock solution was prepared in phosphate-buffered saline (PBS) at 15 mg/ml. To produce bioluminescence, infected and control mice received an intraperitoneal injection of luciferin stock solution (150 mg/kg). All mice were immediately anesthetized with 2% isofluorane, and after 10 minutes were imaged. Ex vivo evaluation of infected tissues and organs was evaluated in a 24-well plate in 150 μg/ml D-luciferin diluted in PBS. Images were captured using the IVIS Lumina image system (Xenogen). Dissected organs were also evaluated by microscopy of hematoxylin-eosin stained sections. Results: Here we describe the results obtained using a genetically modified Dm28c strain of T. cruzi expressing the firefly luciferase to keep track of infection by bioluminescence imaging. Progression of infection was observed in vivo in BALB/c mice at various intervals after infection with transgenic Dm28c-luc. The bioluminescent signal was immediately observed at the site of T. cruzi inoculation, and one day post infection (dpi) it was disseminated in the peritoneal cavity. A similar pattern in the cavity was observed on 7 dpi, but the bioluminescence was more intense in the terminal region of the large intestine, rectum, and gonads. On 14 and 21 dpi, bioluminescent parasites were also observed in the heart, snout, paws, hind limbs, and forelimbs. From 28 dpi to 180 dpi in chronically infected mice, bioluminescence declined in regions of the body but was concentrated in the gonad region. Ex vivo evaluation of dissected organs and tissues by bioluminescent imaging confirmed the in vivo bioluminescent foci. Histopathological analysis of dissected organs demonstrated parasite nests at the rectum and snout, in muscle fibers of mice infected with Dm28c-WT and with Dm28c-luc, corroborating the bioluminescent imaging. Conclusion: Bioluminescence imaging is accurate for tracking parasites in vivo, and this methodology is important to gain a better understanding of the infection, tissue inflammation, and parasite biology regarding host cell interaction, proliferation, and parasite clearance to subpatent levels.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23948/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALaline_ribeiro_etal_IOC_2014.pdfaline_ribeiro_etal_IOC_2014.pdfapplication/pdf8110915https://www.arca.fiocruz.br/bitstream/icict/23948/2/aline_ribeiro_etal_IOC_2014.pdf9a1a9395a297903486c3ace5ee063bccMD52TEXTaline_ribeiro_etal_IOC_2014.pdf.txtaline_ribeiro_etal_IOC_2014.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
In vivo imaging of mice infected with bioluminescent Trypanosoma cruzi unveils novel sites of infection |
title |
In vivo imaging of mice infected with bioluminescent Trypanosoma cruzi unveils novel sites of infection |
spellingShingle |
In vivo imaging of mice infected with bioluminescent Trypanosoma cruzi unveils novel sites of infection Henriques, Cristina Trypanosoma cruzi Doença de Chagas Luciferases Bioluminiscencia Testículo Reto Coração Camundongos Trypanosoma cruzi Chagas Disease Luciferase Bioluminescence Testicles Rectum Heart Mice |
title_short |
In vivo imaging of mice infected with bioluminescent Trypanosoma cruzi unveils novel sites of infection |
title_full |
In vivo imaging of mice infected with bioluminescent Trypanosoma cruzi unveils novel sites of infection |
title_fullStr |
In vivo imaging of mice infected with bioluminescent Trypanosoma cruzi unveils novel sites of infection |
title_full_unstemmed |
In vivo imaging of mice infected with bioluminescent Trypanosoma cruzi unveils novel sites of infection |
title_sort |
In vivo imaging of mice infected with bioluminescent Trypanosoma cruzi unveils novel sites of infection |
author |
Henriques, Cristina |
author_facet |
Henriques, Cristina Pons, Andréa Henriques Batista, Marcelo Meuser Ribeiro, Aline Salgado Souza, Wanderley de |
author_role |
author |
author2 |
Pons, Andréa Henriques Batista, Marcelo Meuser Ribeiro, Aline Salgado Souza, Wanderley de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Henriques, Cristina Pons, Andréa Henriques Batista, Marcelo Meuser Ribeiro, Aline Salgado Souza, Wanderley de |
dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma cruzi Doença de Chagas Luciferases Bioluminiscencia Testículo Reto Coração Camundongos |
topic |
Trypanosoma cruzi Doença de Chagas Luciferases Bioluminiscencia Testículo Reto Coração Camundongos Trypanosoma cruzi Chagas Disease Luciferase Bioluminescence Testicles Rectum Heart Mice |
dc.subject.en.pt_BR.fl_str_mv |
Trypanosoma cruzi Chagas Disease Luciferase Bioluminescence Testicles Rectum Heart Mice |
description |
Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Biomagens. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. CENABIO. Núcleo de Biologia Estrutural e Bioimagens. Rio de Janeiro, RJ,, Brasil / Fundação Oswaldo Cruz. Campo Grande, MS, Brasil. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014 |
dc.date.accessioned.fl_str_mv |
2018-01-18T14:08:56Z |
dc.date.available.fl_str_mv |
2018-01-18T14:08:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
HENRIQUES, Cristina; et al. In vivo imaging of mice infected with bioluminescent Trypanosoma cruzi unveils novel sites of infection. Parasites & Vectors, v.7:89, 15p, 2014. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/23948 |
dc.identifier.issn.pt_BR.fl_str_mv |
1756-3305 |
dc.identifier.doi.none.fl_str_mv |
10.1186/1756-3305-7-89 |
identifier_str_mv |
HENRIQUES, Cristina; et al. In vivo imaging of mice infected with bioluminescent Trypanosoma cruzi unveils novel sites of infection. Parasites & Vectors, v.7:89, 15p, 2014. 1756-3305 10.1186/1756-3305-7-89 |
url |
https://www.arca.fiocruz.br/handle/icict/23948 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
BioMed Central |
publisher.none.fl_str_mv |
BioMed Central |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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FIOCRUZ |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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Repositório Institucional da FIOCRUZ (ARCA) |
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