Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2008 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/1961 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
| id |
CRUZ_7515cb53456598f133955e822c85b078 |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/1961 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Oliveira, Sheilla Andrade deSouza, Bruno Solano de FreitasFerreira, Carla Adriana GuimarãesBarreto, Elton Pereira SáSouza, Siane Campos deFreitas, Luiz Antonio Rodrigues deSantos, Ricardo Ribeiro dosSoares, Milena Botelho Pereira2011-04-25T19:19:15Z2011-04-25T19:19:15Z2008OLIVEIRA, S. A. et al. Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni. World Journal of Gastroenterology. v.14, n.38, p.5842-5850, Oct., 2009.1007-9327https://www.arca.fiocruz.br/handle/icict/196110.3748/wjg.14.5842engTherapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoniinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilTo investigate the potential of bone marrow mononuclear cells (BM-MCs) in the regeneration of hepatic lesions induced by Schistosoma mansoni (S.mansoni) chronic infection.Female mice chronically infected with S.mansoni were treated with BM-MCs obtained from male green fluorescent protein (GFP) transgenic mice by intravenous or intralobular injections. Control mice received injections of saline in similar conditions. Enzyme-linked immunosorbent assay (ELISA) assay for transforming growth factor-beta (TGF-beta), polymerase chain reaction (PCR) for GFP DNA, immunofluorescence and morphometric studies were performed.Transplanted GFP(+) cells migrated to granuloma areas and reduced the percentage of liver fibrosis. The presence of donor-derived cells was confirmed by fluorescence in situ hybridization (FISH) analysis for detection of cells bearing Y chromosome and by PCR analysis for detection of GFP DNA. The levels of TGF-beta, a cytokine associated with fibrosis deposition, in liver fragments of mice submitted to therapy were reduced. The number of oval cells in liver sections of S.mansoni-infected mice increased 3-4 fold after transplantation. A partial recovery in albumin expression, which is decreased upon infection with S.mansoni, was found in livers of infected mice after cellular therapy.In conclusion, transplanted BMCs migrate to and reduce the damage of chronic fibrotic liver lesions caused by S.mansoniSchistosoma mansoniLiver injuryFibrosisBone marrow cellsTransplantationAlbuminOval cellsCélulas da Medula ÓsseaCirrose Hepática ExperimentalTransplante de Medula ÓsseaFígadoRegeneração HepáticaSchistosoma mansoniEsquistossomose mansoniCamundongos Endogâmicos C57BLinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALTherapy with bone marrow cells reduces liver.pdfTherapy with bone marrow cells reduces liver.pdfapplication/pdf2330871https://www.arca.fiocruz.br/bitstream/icict/1961/1/Therapy%20with%20bone%20marrow%20cells%20reduces%20liver.pdf39cf4bfa8a40780c2536ab2b7c1cfc47MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/1961/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTTherapy with bone marrow cells reduces liver.pdf.txtTherapy with bone marrow cells reduces liver.pdf.txtExtracted texttext/plain41128https://www.arca.fiocruz.br/bitstream/icict/1961/5/Therapy%20with%20bone%20marrow%20cells%20reduces%20liver.pdf.txt268ce695cdd31e8b6a18b3182a8f4127MD55THUMBNAILTherapy with bone marrow cells reduces liver.pdf.jpgTherapy with bone marrow cells reduces liver.pdf.jpgGenerated Thumbnailimage/jpeg2286https://www.arca.fiocruz.br/bitstream/icict/1961/4/Therapy%20with%20bone%20marrow%20cells%20reduces%20liver.pdf.jpg8c654f34b089e3c224c31db44c51d6aaMD54icict/19612023-03-15 14:33:03.451oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:03Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni |
| title |
Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni |
| spellingShingle |
Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni Oliveira, Sheilla Andrade de Schistosoma mansoni Liver injury Fibrosis Bone marrow cells Transplantation Albumin Oval cells Células da Medula Óssea Cirrose Hepática Experimental Transplante de Medula Óssea Fígado Regeneração Hepática Schistosoma mansoni Esquistossomose mansoni Camundongos Endogâmicos C57BL |
| title_short |
Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni |
| title_full |
Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni |
| title_fullStr |
Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni |
| title_full_unstemmed |
Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni |
| title_sort |
Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni |
| author |
Oliveira, Sheilla Andrade de |
| author_facet |
Oliveira, Sheilla Andrade de Souza, Bruno Solano de Freitas Ferreira, Carla Adriana Guimarães Barreto, Elton Pereira Sá Souza, Siane Campos de Freitas, Luiz Antonio Rodrigues de Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
| author_role |
author |
| author2 |
Souza, Bruno Solano de Freitas Ferreira, Carla Adriana Guimarães Barreto, Elton Pereira Sá Souza, Siane Campos de Freitas, Luiz Antonio Rodrigues de Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Oliveira, Sheilla Andrade de Souza, Bruno Solano de Freitas Ferreira, Carla Adriana Guimarães Barreto, Elton Pereira Sá Souza, Siane Campos de Freitas, Luiz Antonio Rodrigues de Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
| dc.subject.en.pt_BR.fl_str_mv |
Schistosoma mansoni Liver injury Fibrosis Bone marrow cells Transplantation Albumin Oval cells |
| topic |
Schistosoma mansoni Liver injury Fibrosis Bone marrow cells Transplantation Albumin Oval cells Células da Medula Óssea Cirrose Hepática Experimental Transplante de Medula Óssea Fígado Regeneração Hepática Schistosoma mansoni Esquistossomose mansoni Camundongos Endogâmicos C57BL |
| dc.subject.decs.pt_BR.fl_str_mv |
Células da Medula Óssea Cirrose Hepática Experimental Transplante de Medula Óssea Fígado Regeneração Hepática Schistosoma mansoni Esquistossomose mansoni Camundongos Endogâmicos C57BL |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil |
| publishDate |
2008 |
| dc.date.issued.fl_str_mv |
2008 |
| dc.date.accessioned.fl_str_mv |
2011-04-25T19:19:15Z |
| dc.date.available.fl_str_mv |
2011-04-25T19:19:15Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
OLIVEIRA, S. A. et al. Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni. World Journal of Gastroenterology. v.14, n.38, p.5842-5850, Oct., 2009. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/1961 |
| dc.identifier.issn.none.fl_str_mv |
1007-9327 |
| dc.identifier.doi.none.fl_str_mv |
10.3748/wjg.14.5842 |
| identifier_str_mv |
OLIVEIRA, S. A. et al. Therapy with bone marrow cells reduces liver alterations in mice chronically infected by Schistosoma mansoni. World Journal of Gastroenterology. v.14, n.38, p.5842-5850, Oct., 2009. 1007-9327 10.3748/wjg.14.5842 |
| url |
https://www.arca.fiocruz.br/handle/icict/1961 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/1961/1/Therapy%20with%20bone%20marrow%20cells%20reduces%20liver.pdf https://www.arca.fiocruz.br/bitstream/icict/1961/2/license.txt https://www.arca.fiocruz.br/bitstream/icict/1961/5/Therapy%20with%20bone%20marrow%20cells%20reduces%20liver.pdf.txt https://www.arca.fiocruz.br/bitstream/icict/1961/4/Therapy%20with%20bone%20marrow%20cells%20reduces%20liver.pdf.jpg |
| bitstream.checksum.fl_str_mv |
39cf4bfa8a40780c2536ab2b7c1cfc47 e095249ac7cacefbfe39684dfe45e706 268ce695cdd31e8b6a18b3182a8f4127 8c654f34b089e3c224c31db44c51d6aa |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1813009284629266432 |