Screening of Dengue virus antiviral activity of marine seaweeds by an in situ enzyme-linked immunosorbent assay
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/38844 |
Resumo: | Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. |
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Koishi, Andrea CristineZanello, Paula RodriguesBianco, Éverson MiguelBordignon, JulianoSantos, Claudia Nunes Duarte dos2019-12-26T14:58:32Z2019-12-26T14:58:32Z2012KOISHI, Andrea Cristine et al. Screening of Dengue virus antiviral activity of marine seaweeds by an in situ enzyme-linked immunosorbent assay. PLoS One, v. 7, n. 12, p. 1-11, 2012.1932-6203https://www.arca.fiocruz.br/handle/icict/3884410.1371/journal.pone.0051089engPublic Library of ScienceScreening of Dengue virus antiviral activity of marine seaweeds by an in situ enzyme-linked immunosorbent assayinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Universidade Federal de Pernambuco. Programa de Pós-Graduação em Química. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Dengue is a significant public health problem worldwide. Despite the important social and clinical impact, there is no vaccine or specific antiviral therapy for prevention and treatment of dengue virus (DENV) infection. Considering the above, drug discovery research for dengue is of utmost importance; in addition natural marine products provide diverse and novel chemical structures with potent biological activities that must be evaluated. In this study we propose a target-free approach for dengue drug discovery based on a novel, rapid, and economic in situ enzyme-linked immunosorbent assay and the screening of a panel of marine seaweed extracts. The in situ ELISA was standardized and validated for Huh7.5 cell line infected with all four serotypes of DENV, among them clinical isolates and a laboratory strain. Statistical analysis showed an average S/B of 7.2 and Z-factor of 0.62, demonstrating assay consistency and reliability. A panel of fifteen seaweed extracts was then screened at the maximum non-toxic dose previously determined by the MTT and Neutral Red cytotoxic assays. Eight seaweed extracts were able to reduce DENV infection of at least one serotype tested. Four extracts (Phaeophyta: Canistrocarpus cervicornis, Padina gymnospora; Rhodophyta: Palisada perforate; Chlorophyta: Caulerpa racemosa) were chosen for further evaluation, and time of addition studies point that they might act at an early stage of the viral infection cycle, such as binding or internalization.Dengue VirusSeaweedAntiviral AgentsPhaeophytaRhodophytaChlorophytaVirus del DengueAlgas MarinasAntiviralesVírus da DengueAlga MarinhaAntiviraisFeófitasRodófitasClorófitasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/38844/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALjournal.pone.0051089ok.PDFjournal.pone.0051089ok.PDFapplication/pdf653262https://www.arca.fiocruz.br/bitstream/icict/38844/2/journal.pone.0051089ok.PDF110e684aa539c27902ac88f742a5be87MD52TEXTjournal.pone.0051089ok.PDF.txtjournal.pone.0051089ok.PDF.txtExtracted texttext/plain49839https://www.arca.fiocruz.br/bitstream/icict/38844/3/journal.pone.0051089ok.PDF.txt261e5c32a1292194c39a250a3bd37e8eMD53icict/388442019-12-30 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| dc.title.pt_BR.fl_str_mv |
Screening of Dengue virus antiviral activity of marine seaweeds by an in situ enzyme-linked immunosorbent assay |
| title |
Screening of Dengue virus antiviral activity of marine seaweeds by an in situ enzyme-linked immunosorbent assay |
| spellingShingle |
Screening of Dengue virus antiviral activity of marine seaweeds by an in situ enzyme-linked immunosorbent assay Koishi, Andrea Cristine Dengue Virus Seaweed Antiviral Agents Phaeophyta Rhodophyta Chlorophyta Virus del Dengue Algas Marinas Antivirales Vírus da Dengue Alga Marinha Antivirais Feófitas Rodófitas Clorófitas |
| title_short |
Screening of Dengue virus antiviral activity of marine seaweeds by an in situ enzyme-linked immunosorbent assay |
| title_full |
Screening of Dengue virus antiviral activity of marine seaweeds by an in situ enzyme-linked immunosorbent assay |
| title_fullStr |
Screening of Dengue virus antiviral activity of marine seaweeds by an in situ enzyme-linked immunosorbent assay |
| title_full_unstemmed |
Screening of Dengue virus antiviral activity of marine seaweeds by an in situ enzyme-linked immunosorbent assay |
| title_sort |
Screening of Dengue virus antiviral activity of marine seaweeds by an in situ enzyme-linked immunosorbent assay |
| author |
Koishi, Andrea Cristine |
| author_facet |
Koishi, Andrea Cristine Zanello, Paula Rodrigues Bianco, Éverson Miguel Bordignon, Juliano Santos, Claudia Nunes Duarte dos |
| author_role |
author |
| author2 |
Zanello, Paula Rodrigues Bianco, Éverson Miguel Bordignon, Juliano Santos, Claudia Nunes Duarte dos |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Koishi, Andrea Cristine Zanello, Paula Rodrigues Bianco, Éverson Miguel Bordignon, Juliano Santos, Claudia Nunes Duarte dos |
| dc.subject.en.pt_BR.fl_str_mv |
Dengue Virus Seaweed Antiviral Agents Phaeophyta Rhodophyta Chlorophyta |
| topic |
Dengue Virus Seaweed Antiviral Agents Phaeophyta Rhodophyta Chlorophyta Virus del Dengue Algas Marinas Antivirales Vírus da Dengue Alga Marinha Antivirais Feófitas Rodófitas Clorófitas |
| dc.subject.es.pt_BR.fl_str_mv |
Virus del Dengue Algas Marinas Antivirales |
| dc.subject.decs.pt_BR.fl_str_mv |
Vírus da Dengue Alga Marinha Antivirais Feófitas Rodófitas Clorófitas |
| description |
Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. |
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2012 |
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2012 |
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2019-12-26T14:58:32Z |
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2019-12-26T14:58:32Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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KOISHI, Andrea Cristine et al. Screening of Dengue virus antiviral activity of marine seaweeds by an in situ enzyme-linked immunosorbent assay. PLoS One, v. 7, n. 12, p. 1-11, 2012. |
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https://www.arca.fiocruz.br/handle/icict/38844 |
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1932-6203 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0051089 |
| identifier_str_mv |
KOISHI, Andrea Cristine et al. Screening of Dengue virus antiviral activity of marine seaweeds by an in situ enzyme-linked immunosorbent assay. PLoS One, v. 7, n. 12, p. 1-11, 2012. 1932-6203 10.1371/journal.pone.0051089 |
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