Effect of mesenchymal stem cells on movement and urination of rats with spinal cord injury
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/22783 |
Resumo: | Pontifícia Universidade Católica do Paraná. Curitiba. PR, Brasil. |
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Silva, Alexandra Justino daVillanova Junior, José AdemarFracaro, LetíciaRebelatto, Carmen Lúcia KuniyoshiBarchiki, FabianeMoura, Sérgio Adriane Bezerra deDominguez, Alejandro CorreaAbud, Ana Paula RessettiLocatelli-Dittrich, RosângelaBrofman, Paulo Roberto SludQuitzan, Juliany Gomes2017-10-05T15:29:01Z2017-10-05T15:29:01Z2014SILVA, Alexandra Justino da. et al. Effect of mesenchymal stem cells on movement and urination of rats with spinal cord injury. Semina: Ciências Agrárias, v. 35, n.6, p. 3205-3214, 2014.1679-0359https://www.arca.fiocruz.br/handle/icict/2278310.5433/1679-0359.2014v35n6p3205porUniversidade Estadual de LondrinaParaplegiaSpinal Cord InjuriesUrinary IncontinenceCell- and Tissue-Based TherapyLuminescent MeasurementsTraumatismos de la Médula EspinalParaplejíaIncontinencia UrinariaTratamiento Basado en Trasplante de Células y TejidosMediciones LuminiscentesTraumatismos da Medula EspinalIncontinência UrináriaTerapia Baseada em Transplante de Células e TecidosMedições LuminescentesEffect of mesenchymal stem cells on movement and urination of rats with spinal cord injuryEfeitos da aplicação das células-tronco mesenquimais sobre a locomoção e micção de ratos submetidos à lesão medularinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlePontifícia Universidade Católica do Paraná. Curitiba. PR, Brasil.Pontifícia Universidade Católica do Paraná. Curitiba. PR, Brasil.Pontifícia Universidade Católica do Paraná. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Curitiba, PR, Brasil.Universidade Federal do Rio Grande Norte. Natal, RN, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Federal do Paraná. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Curitiba. PR, Brasil.Universidade Estadual Paulista. Botucatu, SP, Brasil.Cell therapy has frequently been reported as a possible treatment for spinal trauma in humans and animals; however, without pharmacologically curative action on damage from the primary lesion. In this study, we evaluated the effect of administering human adipose-derived stem cells (hADSC) in rats after spinal cord injury. The hADSC were used between the third and fifth passages and a proportion of cells were transduced for screening in vivo after transplantation. Spinal cord injury was induced with a Fogarty catheter no. 3 inserted into the epidural space with a cuff located at T8 and filled with 80 μL saline for 5 min. The control group A (n = 12) received culture medium (50 μL) and group B (n = 12) received hADSC (1.2 × 106) at 7 and 14 days post-injury, in the tail vein. Emptying of the bladder by massage was performed daily for 3 months. Evaluation of functional motor activity was performed daily until 3 months post-injury using the Basso-Beattie-Bresnahan scale. Subsequently, the animals were euthanized and histological analysis of the urinary bladder and spinal cord was performed. Bioluminescence analysis revealed hADSC at the application site and lungs. There was improvement of urinary bladder function in 83.3% animals in group B and 16.66% animals in group A. The analysis of functional motor activity and histology of the spinal cord and urinary bladder demonstrated no significant difference between groups A and B. The results indicate that transplanted hADSC improved urinary function via a telecrine mechanism, namely action at a distance.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/22783/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALsilvaEffectmesenchymalok.pdfsilvaEffectmesenchymalok.pdfapplication/pdf1177158https://www.arca.fiocruz.br/bitstream/icict/22783/2/silvaEffectmesenchymalok.pdf77cc462b61e1bf2b575201f1162ae4f7MD52TEXTsilvaEffectmesenchymalok.pdf.txtsilvaEffectmesenchymalok.pdf.txtExtracted texttext/plain36272https://www.arca.fiocruz.br/bitstream/icict/22783/3/silvaEffectmesenchymalok.pdf.txt739dd70053ee2b197c934becad054b7aMD53icict/227832018-08-15 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| dc.title.pt_BR.fl_str_mv |
Effect of mesenchymal stem cells on movement and urination of rats with spinal cord injury |
| dc.title.alternative.pt_BR.fl_str_mv |
Efeitos da aplicação das células-tronco mesenquimais sobre a locomoção e micção de ratos submetidos à lesão medular |
| title |
Effect of mesenchymal stem cells on movement and urination of rats with spinal cord injury |
| spellingShingle |
Effect of mesenchymal stem cells on movement and urination of rats with spinal cord injury Silva, Alexandra Justino da Paraplegia Spinal Cord Injuries Urinary Incontinence Cell- and Tissue-Based Therapy Luminescent Measurements Traumatismos de la Médula Espinal Paraplejía Incontinencia Urinaria Tratamiento Basado en Trasplante de Células y Tejidos Mediciones Luminiscentes Traumatismos da Medula Espinal Incontinência Urinária Terapia Baseada em Transplante de Células e Tecidos Medições Luminescentes |
| title_short |
Effect of mesenchymal stem cells on movement and urination of rats with spinal cord injury |
| title_full |
Effect of mesenchymal stem cells on movement and urination of rats with spinal cord injury |
| title_fullStr |
Effect of mesenchymal stem cells on movement and urination of rats with spinal cord injury |
| title_full_unstemmed |
Effect of mesenchymal stem cells on movement and urination of rats with spinal cord injury |
| title_sort |
Effect of mesenchymal stem cells on movement and urination of rats with spinal cord injury |
| author |
Silva, Alexandra Justino da |
| author_facet |
Silva, Alexandra Justino da Villanova Junior, José Ademar Fracaro, Letícia Rebelatto, Carmen Lúcia Kuniyoshi Barchiki, Fabiane Moura, Sérgio Adriane Bezerra de Dominguez, Alejandro Correa Abud, Ana Paula Ressetti Locatelli-Dittrich, Rosângela Brofman, Paulo Roberto Slud Quitzan, Juliany Gomes |
| author_role |
author |
| author2 |
Villanova Junior, José Ademar Fracaro, Letícia Rebelatto, Carmen Lúcia Kuniyoshi Barchiki, Fabiane Moura, Sérgio Adriane Bezerra de Dominguez, Alejandro Correa Abud, Ana Paula Ressetti Locatelli-Dittrich, Rosângela Brofman, Paulo Roberto Slud Quitzan, Juliany Gomes |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Alexandra Justino da Villanova Junior, José Ademar Fracaro, Letícia Rebelatto, Carmen Lúcia Kuniyoshi Barchiki, Fabiane Moura, Sérgio Adriane Bezerra de Dominguez, Alejandro Correa Abud, Ana Paula Ressetti Locatelli-Dittrich, Rosângela Brofman, Paulo Roberto Slud Quitzan, Juliany Gomes |
| dc.subject.other.pt_BR.fl_str_mv |
Paraplegia |
| topic |
Paraplegia Spinal Cord Injuries Urinary Incontinence Cell- and Tissue-Based Therapy Luminescent Measurements Traumatismos de la Médula Espinal Paraplejía Incontinencia Urinaria Tratamiento Basado en Trasplante de Células y Tejidos Mediciones Luminiscentes Traumatismos da Medula Espinal Incontinência Urinária Terapia Baseada em Transplante de Células e Tecidos Medições Luminescentes |
| dc.subject.en.pt_BR.fl_str_mv |
Spinal Cord Injuries Urinary Incontinence Cell- and Tissue-Based Therapy Luminescent Measurements |
| dc.subject.es.pt_BR.fl_str_mv |
Traumatismos de la Médula Espinal Paraplejía Incontinencia Urinaria Tratamiento Basado en Trasplante de Células y Tejidos Mediciones Luminiscentes |
| dc.subject.decs.pt_BR.fl_str_mv |
Traumatismos da Medula Espinal Incontinência Urinária Terapia Baseada em Transplante de Células e Tecidos Medições Luminescentes |
| description |
Pontifícia Universidade Católica do Paraná. Curitiba. PR, Brasil. |
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2014 |
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2014 |
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2017-10-05T15:29:01Z |
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2017-10-05T15:29:01Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
SILVA, Alexandra Justino da. et al. Effect of mesenchymal stem cells on movement and urination of rats with spinal cord injury. Semina: Ciências Agrárias, v. 35, n.6, p. 3205-3214, 2014. |
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https://www.arca.fiocruz.br/handle/icict/22783 |
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1679-0359 |
| dc.identifier.doi.none.fl_str_mv |
10.5433/1679-0359.2014v35n6p3205 |
| identifier_str_mv |
SILVA, Alexandra Justino da. et al. Effect of mesenchymal stem cells on movement and urination of rats with spinal cord injury. Semina: Ciências Agrárias, v. 35, n.6, p. 3205-3214, 2014. 1679-0359 10.5433/1679-0359.2014v35n6p3205 |
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Universidade Estadual de Londrina |
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Universidade Estadual de Londrina |
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