The NtrY–NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/16227 |
Resumo: | INCT-Fixação Biologica de Nitrogênio/CNPq/MCTI, CNPq, CAPES, Fundação Araucaria, Biotechnology and Biological Sciences Research Council (BB/L011468/1). |
| id |
CRUZ_76f60841d958390b27f8184b696d7e02 |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/16227 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Bonato, PalomaAlves, Lysangela RonalteOsaki, Juliana H.Rigo, Liu U.Pedrosa, Fábio OliveiraSouza, Emanuel Maltempi deZhang, NanSchumacher, JorgBuck, MartinWassem, RoseliChubatsu, Leda S.2016-10-13T17:17:07Z2016-10-13T17:17:07Z2016BONATO, Paloma et al. The NtrY–NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1. FEBS Journal, p. 1-12, Sept. 2016.1742-464Xhttps://www.arca.fiocruz.br/handle/icict/1622710.1111/febs.13897INCT-Fixação Biologica de Nitrogênio/CNPq/MCTI, CNPq, CAPES, Fundação Araucaria, Biotechnology and Biological Sciences Research Council (BB/L011468/1).Department of Biochemistry and Molecular Biology. Universidade Federal do Paraná. Curitiba, PR, Brasil.Department of Biochemistry and Molecular Biology. Universidade Federal do Paraná. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Department of Biochemistry and Molecular Biology. Universidade Federal do Paraná. Curitiba, PR, Brasil.Department of Biochemistry and Molecular Biology. Universidade Federal do Paraná. Curitiba, PR, Brasil.Department of Biochemistry and Molecular Biology. Universidade Federal do Paraná. Curitiba, PR, Brasil.Department of Biochemistry and Molecular Biology. Universidade Federal do Paraná. Curitiba, PR, Brasil.Department of Life Sciences, Imperial College London, UKDepartment of Life Sciences, Imperial College London, UKDepartment of Life Sciences, Imperial College London, UKUniversidade Federal do Paraná. Department of Genetics. Curitiba, PR, Brasil.Department of Biochemistry and Molecular Biology. Universidade Federal do Paraná. Curitiba, PR, Brasil.Herbaspirillum seropedicae is a diazotrophic β-Proteobacterium found endophytically associated with gramineae (Poaceae or graminaceous plants) such as rice, sorghum and sugar cane. In this work we show that nitrate-dependent growth in this organism is regulated by the master nitrogen regulatory two-component system NtrB-NtrC, and by NtrY-NtrX, which functions to specifically regulate nitrate metabolism. NtrY is a histidine kinase sensor protein predicted to be associated with the membrane and NtrX is the response regulator partner. The ntrYntrX genes are widely distributed in Proteobacteria. In α-Proteobacteria they are frequently located downstream from ntrBC, whereas in β-Proteobacteria these genes are located downstream from genes encoding an RNA methyltransferase and a proline-rich protein with unknown function. The NtrX protein of α-Proteobacteria has an AAA+ domain, absent in those from β-Proteobacteria. An ntrY mutant of H. seropedicae showed the wild-type nitrogen fixation phenotype, but the nitrate-dependent growth was abolished. Gene fusion assays indicated that NtrY is involved in the expression of genes coding for the assimilatory nitrate reductase as well as the nitrate-responsive two-component system NarX-NarL (narK and narX promoters, respectively). The purified NtrX protein was capable of binding the narK and narX promoters, and the binding site at the narX promoter for the NtrX protein was determined by DNA footprinting. In silico analyses revealed similar sequences in other promoter regions of H. seropedicae that are related to nitrate assimilation, supporting the role of the NtrY-NtrX system in regulating nitrate metabolism in H. seropedicae.engSeamus MartinThe NtrY–NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHerbaspirillum seropedicaeNitrate metabolismNtrXNtrYTwo-component systemNitrato RedutaseMetabolismoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/16227/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALThe NtrY–NtrX two-component system.pdfThe NtrY–NtrX two-component system.pdfapplication/pdf919572https://www.arca.fiocruz.br/bitstream/icict/16227/2/The%20NtrY%e2%80%93NtrX%20two-component%20system.pdf2fa21e44abff448bcff5c6989d5353bdMD52TEXTThe NtrY–NtrX two-component system.pdf.txtThe NtrY–NtrX two-component system.pdf.txtExtracted texttext/plain47419https://www.arca.fiocruz.br/bitstream/icict/16227/3/The%20NtrY%e2%80%93NtrX%20two-component%20system.pdf.txt5f9a5a59f266b8d8429995ae62a76be4MD53icict/162272022-10-20 18:58:38.054oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-10-20T21:58:38Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
The NtrY–NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1 |
| title |
The NtrY–NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1 |
| spellingShingle |
The NtrY–NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1 Bonato, Paloma Herbaspirillum seropedicae Nitrate metabolism NtrX NtrY Two-component system Nitrato Redutase Metabolismo |
| title_short |
The NtrY–NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1 |
| title_full |
The NtrY–NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1 |
| title_fullStr |
The NtrY–NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1 |
| title_full_unstemmed |
The NtrY–NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1 |
| title_sort |
The NtrY–NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1 |
| author |
Bonato, Paloma |
| author_facet |
Bonato, Paloma Alves, Lysangela Ronalte Osaki, Juliana H. Rigo, Liu U. Pedrosa, Fábio Oliveira Souza, Emanuel Maltempi de Zhang, Nan Schumacher, Jorg Buck, Martin Wassem, Roseli Chubatsu, Leda S. |
| author_role |
author |
| author2 |
Alves, Lysangela Ronalte Osaki, Juliana H. Rigo, Liu U. Pedrosa, Fábio Oliveira Souza, Emanuel Maltempi de Zhang, Nan Schumacher, Jorg Buck, Martin Wassem, Roseli Chubatsu, Leda S. |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Bonato, Paloma Alves, Lysangela Ronalte Osaki, Juliana H. Rigo, Liu U. Pedrosa, Fábio Oliveira Souza, Emanuel Maltempi de Zhang, Nan Schumacher, Jorg Buck, Martin Wassem, Roseli Chubatsu, Leda S. |
| dc.subject.en.pt_BR.fl_str_mv |
Herbaspirillum seropedicae Nitrate metabolism NtrX NtrY Two-component system |
| topic |
Herbaspirillum seropedicae Nitrate metabolism NtrX NtrY Two-component system Nitrato Redutase Metabolismo |
| dc.subject.decs.pt_BR.fl_str_mv |
Nitrato Redutase Metabolismo |
| description |
INCT-Fixação Biologica de Nitrogênio/CNPq/MCTI, CNPq, CAPES, Fundação Araucaria, Biotechnology and Biological Sciences Research Council (BB/L011468/1). |
| publishDate |
2016 |
| dc.date.accessioned.fl_str_mv |
2016-10-13T17:17:07Z |
| dc.date.available.fl_str_mv |
2016-10-13T17:17:07Z |
| dc.date.issued.fl_str_mv |
2016 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
BONATO, Paloma et al. The NtrY–NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1. FEBS Journal, p. 1-12, Sept. 2016. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/16227 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1742-464X |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1111/febs.13897 |
| identifier_str_mv |
BONATO, Paloma et al. The NtrY–NtrX two-component system is involved in controlling nitrate assimilation in Herbaspirillum seropedicae strain SmR1. FEBS Journal, p. 1-12, Sept. 2016. 1742-464X 10.1111/febs.13897 |
| url |
https://www.arca.fiocruz.br/handle/icict/16227 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Seamus Martin |
| publisher.none.fl_str_mv |
Seamus Martin |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/16227/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/16227/2/The%20NtrY%e2%80%93NtrX%20two-component%20system.pdf https://www.arca.fiocruz.br/bitstream/icict/16227/3/The%20NtrY%e2%80%93NtrX%20two-component%20system.pdf.txt |
| bitstream.checksum.fl_str_mv |
0271fb68963c017aa933c7aff60379f4 2fa21e44abff448bcff5c6989d5353bd 5f9a5a59f266b8d8429995ae62a76be4 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1813009309634658304 |