miRNA Expression in Control and FSHD Fetal Human Muscle Biopsies

Detalhes bibliográficos
Autor(a) principal: Portilho, Débora Morueco
Data de Publicação: 2015
Outros Autores: Alves, Marcelo Ribeiro, Kratassiouk, Gueorgui, Roche, Stéphane, Magdinier, Frédérique, Santana, Eliane Corrêa de, Polesskaya, Anna, Harel-Bellan, Annick, Mouly, Vincent, Savino, Wilson, Butler-Browne, Gillian, Dumonceaux, Julie
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11494
Resumo: Sorbonne Universités, UPMC Univ Paris 06, Center of Research in Myology UM76, Paris, France / INSERM UMRS974. Paris, France / CNRS FRE 3617, F-75013. Paris, France / Institut de Myologie, F-75013. Paris, France / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o o Timo. Rio de Janeiro, RJ, Brasil.
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spelling Portilho, Débora MoruecoAlves, Marcelo RibeiroKratassiouk, GueorguiRoche, StéphaneMagdinier, FrédériqueSantana, Eliane Corrêa dePolesskaya, AnnaHarel-Bellan, AnnickMouly, VincentSavino, WilsonButler-Browne, GillianDumonceaux, Julie2015-08-19T13:49:22Z2015-08-19T13:49:22Z2015PORTILHO, Débora Morueco et al. miRNA Expression in Control and FSHD Fetal Human Muscle Biopsies. Plos One, v.10, n. 2, p. 1-12, 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/1149410.1371/journal.pone.0116853engPlos OnemiRNA Expression in Control and FSHD Fetal Human Muscle Biopsiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSorbonne Universités, UPMC Univ Paris 06, Center of Research in Myology UM76, Paris, France / INSERM UMRS974. Paris, France / CNRS FRE 3617, F-75013. Paris, France / Institut de Myologie, F-75013. Paris, France / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto de Pesquisa de Clínica Evandro Chagas. Laboratório de Pesquisa Clínica em DST-AIDS. Rio de Janeiro, RJ, Brasil.CNRS FRE 3377, Université Paris-Sud CEA Saclay, Gif-sur-Yvette, France.Aix Marseille Université. Faculté de Médecine de la Timone. Laboratoire de Génétique Médicale et Génomique Fonctionnelle, INSERM UMRS 910. Marseille, France.Aix Marseille Université. Faculté de Médecine de la Timone. Laboratoire de Génétique Médicale et Génomique Fonctionnelle, INSERM UMRS 910. Marseille, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.CNRS FRE 3377, Université Paris-Sud CEA Saclay, Gif-sur-Yvette, France.CNRS FRE 3377, Université Paris-Sud CEA Saclay, Gif-sur-Yvette, France.Sorbonne Universités, UPMC Univ Paris 06, Center of Research in Myology UM76, Paris, France / INSERM UMRS974. Paris, France / CNRS FRE 3617, F-75013. Paris, France / Institut de Myologie, F-75013. Paris, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o Timo. Rio de Janeiro, RJ, Brasil.Sorbonne Universités, UPMC Univ Paris 06, Center of Research in Myology UM76, Paris, France / INSERM UMRS974. Paris, France / CNRS FRE 3617, F-75013. Paris, France / Institut de Myologie, F-75013. Paris, France.Sorbonne Universités, UPMC Univ Paris 06, Center of Research in Myology UM76, Paris, France / INSERM UMRS974. Paris, France / CNRS FRE 3617, F-75013. Paris, France / Institut de Myologie, F-75013. Paris, France.BACKGROUND: Facioscapulohumeral muscular dystrophy (FSHD) is an autosomal-dominant disorder and is one of the most common forms of muscular dystrophy. We have recently shown that some hallmarks of FSHD are already expressed in fetal FSHD biopsies, thus opening a new field of investigation for mechanisms leading to FSHD. As microRNAs (miRNAs) play an important role in myogenesis and muscle disorders, in this study we compared miRNAs expression levels during normal and FSHD muscle development. METHODS: Muscle biopsies were obtained from quadriceps of both healthy control and FSHD1 fetuses with ages ranging from 14 to 33 weeks of development. miRNA expression profiles were analyzed using TaqMan Human MicroRNA Arrays. RESULTS: During human skeletal muscle development, in control muscle biopsies we observed changes for 4 miRNAs potentially involved in secondary muscle fiber formation and 5 miRNAs potentially involved in fiber maturation. When we compared the miRNA profiles obtained from control and FSHD biopsies, we did not observe any differences in the muscle specific miRNAs. However, we identified 8 miRNAs exclusively expressed in FSHD1 samples (miR-330, miR-331-5p, miR-34a, miR-380-3p, miR-516b, miR-582-5p, miR-517* and miR-625) which could represent new biomarkers for this disease. Their putative targets are mainly involved in muscle development and morphogenesis. Interestingly, these FSHD1 specific miRNAs do not target the genes previously described to be involved in FSHD. CONCLUSIONS: This work provides new candidate mechanisms potentially involved in the onset of FSHD pathology. Whether these FSHD specific miRNAs cause deregulations during fetal development, or protect against the appearance of the FSHD phenotype until the second decade of life still needs to be investigated.miRNABiopsiesFetal developmentFSHDFacioscapulohumeral muscular dystrophyMicroRNAsDistrofia Muscular FacioescapuloumeralBiópsiaDesenvolvimento Fetalinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11494/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALmarcelo_alves_etal_IOC_2015.pdfapplication/pdf1212937https://www.arca.fiocruz.br/bitstream/icict/11494/2/marcelo_alves_etal_IOC_2015.pdff7b95b0f206c7389f4ead4c7c419cf95MD52TEXTmarcelo_alves_etal_IOC_2015.pdf.txtmarcelo_alves_etal_IOC_2015.pdf.txtExtracted texttext/plain56972https://www.arca.fiocruz.br/bitstream/icict/11494/3/marcelo_alves_etal_IOC_2015.pdf.txtef4f5da0be85182d7b8d3d9feef60707MD53icict/114942022-06-24 13:09:04.211oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:09:04Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv miRNA Expression in Control and FSHD Fetal Human Muscle Biopsies
title miRNA Expression in Control and FSHD Fetal Human Muscle Biopsies
spellingShingle miRNA Expression in Control and FSHD Fetal Human Muscle Biopsies
Portilho, Débora Morueco
miRNA
Biopsies
Fetal development
FSHD
Facioscapulohumeral muscular dystrophy
MicroRNAs
Distrofia Muscular Facioescapuloumeral
Biópsia
Desenvolvimento Fetal
title_short miRNA Expression in Control and FSHD Fetal Human Muscle Biopsies
title_full miRNA Expression in Control and FSHD Fetal Human Muscle Biopsies
title_fullStr miRNA Expression in Control and FSHD Fetal Human Muscle Biopsies
title_full_unstemmed miRNA Expression in Control and FSHD Fetal Human Muscle Biopsies
title_sort miRNA Expression in Control and FSHD Fetal Human Muscle Biopsies
author Portilho, Débora Morueco
author_facet Portilho, Débora Morueco
Alves, Marcelo Ribeiro
Kratassiouk, Gueorgui
Roche, Stéphane
Magdinier, Frédérique
Santana, Eliane Corrêa de
Polesskaya, Anna
Harel-Bellan, Annick
Mouly, Vincent
Savino, Wilson
Butler-Browne, Gillian
Dumonceaux, Julie
author_role author
author2 Alves, Marcelo Ribeiro
Kratassiouk, Gueorgui
Roche, Stéphane
Magdinier, Frédérique
Santana, Eliane Corrêa de
Polesskaya, Anna
Harel-Bellan, Annick
Mouly, Vincent
Savino, Wilson
Butler-Browne, Gillian
Dumonceaux, Julie
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Portilho, Débora Morueco
Alves, Marcelo Ribeiro
Kratassiouk, Gueorgui
Roche, Stéphane
Magdinier, Frédérique
Santana, Eliane Corrêa de
Polesskaya, Anna
Harel-Bellan, Annick
Mouly, Vincent
Savino, Wilson
Butler-Browne, Gillian
Dumonceaux, Julie
dc.subject.en.pt_BR.fl_str_mv miRNA
Biopsies
Fetal development
FSHD
Facioscapulohumeral muscular dystrophy
topic miRNA
Biopsies
Fetal development
FSHD
Facioscapulohumeral muscular dystrophy
MicroRNAs
Distrofia Muscular Facioescapuloumeral
Biópsia
Desenvolvimento Fetal
dc.subject.decs.pt_BR.fl_str_mv MicroRNAs
Distrofia Muscular Facioescapuloumeral
Biópsia
Desenvolvimento Fetal
description Sorbonne Universités, UPMC Univ Paris 06, Center of Research in Myology UM76, Paris, France / INSERM UMRS974. Paris, France / CNRS FRE 3617, F-75013. Paris, France / Institut de Myologie, F-75013. Paris, France / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisas sobre o o Timo. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.accessioned.fl_str_mv 2015-08-19T13:49:22Z
dc.date.available.fl_str_mv 2015-08-19T13:49:22Z
dc.date.issued.fl_str_mv 2015
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PORTILHO, Débora Morueco et al. miRNA Expression in Control and FSHD Fetal Human Muscle Biopsies. Plos One, v.10, n. 2, p. 1-12, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11494
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pone.0116853
identifier_str_mv PORTILHO, Débora Morueco et al. miRNA Expression in Control and FSHD Fetal Human Muscle Biopsies. Plos One, v.10, n. 2, p. 1-12, 2015.
1932-6203
10.1371/journal.pone.0116853
url https://www.arca.fiocruz.br/handle/icict/11494
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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