Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/31726 |
Resumo: | Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Niterói, RJ, Brasil. |
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Peralta, Regina Helena SaramagoVelásquez, Jorge NéstorCunha, Flavia de SouzaPantano, María LauraSodré, Fernando CamposSilva, Sidnei daAstudillo, Osvaldo GermánPeralta, José MauroCarnevale, Silvana2019-02-19T11:42:12Z2019-02-19T11:42:12Z2016PERALTA, Regina Helena Saramago et al. Cryptosporidium diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 111, n.1, p. 30-36, Jan. 2016.0074-0276https://www.arca.fiocruz.br/handle/icict/3172610.1590/0074-027601503031678-8060engInstituto Oswaldo CruzGenetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentinainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Niterói, RJ, Brasil.Hospital Municipal de Infecciosas Dr. Francisco Javier Muñiz. Buenos Aires, Argentina.Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Niterói, RJ, Brasil.Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos G. Malbrán. Instituto Nacional de Enfermedades Infecciosas. Buenos Aires, Argentina.Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Parasitologia. Rio de Janeiro, RJ, Brasil.Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos G. Malbrán. Instituto Nacional de Enfermedades Infecciosas. Buenos Aires, Argentina.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Prof. Paulo de Góes. Rio de Janeiro, RJ, Brasil .Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos G. Malbrán. Instituto Nacional de Enfermedades Infecciosas. Buenos Aires, Argentina / Consejo Nacional de Investigaciones Científicas y Técnicas. Buenos Aires, Argentina.The identification and characterisation of Cryptosporidium genotypes and subtypes are fundamental to the study of cryptosporidiosis epidemiology, aiding in prevention and control strategies. The objective was to determine the genetic diversity of Cryptosporidium in samples obtained from hospitals of Rio de Janeiro, Brazil, and Buenos Aires, Argentina. Samples were analysed by microscopy and TaqMan polymerase chain reaction (PCR) assays for Cryptosporidium detection, genotyped by nested-PCR-restriction fragment length polymorphism (RFLP) analysis of the 18S rRNA gene and subtyped by DNA sequencing of the gp60 gene. Among the 89 samples from Rio de Janeiro, Cryptosporidium spp were detected in 26 by microscopy/TaqMan PCR. In samples from Buenos Aires,Cryptosporidium was diagnosed in 15 patients of the 132 studied. The TaqMan PCR and the nested-PCR-RFLP detected Cryptosporidium parvum, Cryptosporidium hominis, and co-infections of both species. In Brazilian samples, the subtypes IbA10G2 and IIcA5G3 were observed. The subtypes found in Argentinean samples were IbA10G2, IaA10G1R4, IaA11G1R4, and IeA11G3T3, and mixed subtypes of Ia and IIa families were detected in the co-infections. C. hominis was the species more frequently detected, and subtype family Ib was reported in both countries. Subtype diversity was higher in Buenos Aires than in Rio de Janeiro and two new subtypes were described for the first time.CryptosporidiumGenotypesGP60 subtypesPCRinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31726/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Peralta_Regina_etal_INI_2016.pdfve_Peralta_Regina_etal_INI_2016.pdfapplication/pdf323701https://www.arca.fiocruz.br/bitstream/icict/31726/2/ve_Peralta_Regina_etal_INI_2016.pdfb890914ec56ae287c5e38da316b218f6MD52TEXTve_Peralta_Regina_etal_INI_2016.pdf.txtve_Peralta_Regina_etal_INI_2016.pdf.txtExtracted texttext/plain41886https://www.arca.fiocruz.br/bitstream/icict/31726/3/ve_Peralta_Regina_etal_INI_2016.pdf.txt0bba9ef711c14e70e70bd6175602e851MD53icict/317262019-02-22 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| dc.title.pt_BR.fl_str_mv |
Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina |
| title |
Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina |
| spellingShingle |
Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina Peralta, Regina Helena Saramago Cryptosporidium Genotypes GP60 subtypes PCR |
| title_short |
Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina |
| title_full |
Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina |
| title_fullStr |
Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina |
| title_full_unstemmed |
Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina |
| title_sort |
Genetic diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina |
| author |
Peralta, Regina Helena Saramago |
| author_facet |
Peralta, Regina Helena Saramago Velásquez, Jorge Néstor Cunha, Flavia de Souza Pantano, María Laura Sodré, Fernando Campos Silva, Sidnei da Astudillo, Osvaldo Germán Peralta, José Mauro Carnevale, Silvana |
| author_role |
author |
| author2 |
Velásquez, Jorge Néstor Cunha, Flavia de Souza Pantano, María Laura Sodré, Fernando Campos Silva, Sidnei da Astudillo, Osvaldo Germán Peralta, José Mauro Carnevale, Silvana |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Peralta, Regina Helena Saramago Velásquez, Jorge Néstor Cunha, Flavia de Souza Pantano, María Laura Sodré, Fernando Campos Silva, Sidnei da Astudillo, Osvaldo Germán Peralta, José Mauro Carnevale, Silvana |
| dc.subject.en.pt_BR.fl_str_mv |
Cryptosporidium Genotypes GP60 subtypes PCR |
| topic |
Cryptosporidium Genotypes GP60 subtypes PCR |
| description |
Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Niterói, RJ, Brasil. |
| publishDate |
2016 |
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2016 |
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2019-02-19T11:42:12Z |
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2019-02-19T11:42:12Z |
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info:eu-repo/semantics/article |
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PERALTA, Regina Helena Saramago et al. Cryptosporidium diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 111, n.1, p. 30-36, Jan. 2016. |
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https://www.arca.fiocruz.br/handle/icict/31726 |
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0074-0276 |
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10.1590/0074-02760150303 |
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1678-8060 |
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PERALTA, Regina Helena Saramago et al. Cryptosporidium diversity of Cryptosporidium identified in clinical samples from cities in Brazil and Argentina. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 111, n.1, p. 30-36, Jan. 2016. 0074-0276 10.1590/0074-02760150303 1678-8060 |
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Instituto Oswaldo Cruz |
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Instituto Oswaldo Cruz |
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