ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/12954 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. |
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Lara, Flavio AlvesPohl, Paula C.Gandara, Ana CarolineFerreira, Jessica da SilvaSilva, Maria Clara NascimentoBechara, Gervásio HenriqueSorgine, Marcos H. F.Almeida, Igor C.Vaz Junior, Itabajara da SilvaOliveira, Pedro L.2016-03-03T13:21:47Z2016-03-03T13:21:47Z2015LARA, Flavio Alves; et al. ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells. Plos One, v.10, n.8, 20p, Aug. 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/1295410.1371/journal.pone.0134779engPublic Library of ScienceATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Centro de Biotecnologia. Porto Alegre, RS, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Rio de Janeiro, RJ, Brasil.Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Patologia Veterinária. Jaboticabal, SP, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular–INCTEM. Rio de Janeiro, RJ, Brasil.University of Texas at El Paso. The Border Biomedical Research Center. El Paso, Texas, USA.Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Centro de Biotecnologia. Porto Alegre, RS, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular–INCTEM. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular–INCTEM. Rio de Janeiro, RJ, Brasil.In ticks, the digestion of blood occurs intracellularly and proteolytic digestion of hemoglobin takes place in a dedicated type of lysosome, the digest vesicle, followed by transfer of the heme moiety of hemoglobin to a specialized organelle that accumulates large heme aggregates, called hemosomes. In the present work, we studied the uptake of fluorescent metalloporphyrins, used as heme analogs, and amitraz, one of the most regularly used acaricides to control cattle tick infestations, by Rhipicephalus (Boophilus) microplus midgut cells. Both compounds were taken up by midgut cells in vitro and accumulated inside the hemosomes. Transport of both molecules was sensitive to cyclosporine A (CsA), a wellknown inhibitor of ATP binding cassette (ABC) transporters. Rhodamine 123, a fluorescent probe that is also a recognized ABC substrate, was similarly directed to the hemosome in a CsA-sensitive manner. Using an antibody against conserved domain of PgP-1-type ABC transporter, we were able to immunolocalize PgP-1 in the digest vesicle membranes. Comparison between two R. microplus strains that were resistant and susceptible to amitraz revealed that the resistant strain detoxified both amitraz and Sn-Pp IX more efficiently than the susceptible strain, a process that was also sensitive to CsA. A transcript containing an ABC transporter signature exhibited 2.5-fold increased expression in the amitraz-resistant strain when compared with the susceptible strain. RNAi-induced down-regulation of this ABC transporter led to the accumulation of metalloporphyrin in the digestive vacuole, interrupting heme traffic to the hemosome. This evidence further confirms that this transcript codes for a heme transporter. This is the first report of heme transport in a blood-feeding organism. While the primary physiological function of the hemosome is to detoxify heme and attenuate its toxicity, we suggest that the use of this acaricide detoxification pathway by ticks may represent a new molecular mechanism of resistance to pesticides.Tick Midgut CellsPesticide DetoxificationATP Binding Cassette TransporterHemeTrifosfato de AdenosinaTransportadores de Cassetes de Ligação de ATPCarrapatosPesticidasHemeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12954/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALflavio_lara_etal_IOC_2015.PDFflavio_lara_etal_IOC_2015.PDFapplication/pdf6748083https://www.arca.fiocruz.br/bitstream/icict/12954/2/flavio_lara_etal_IOC_2015.PDF0febe6bad842ab7fb38d1aad3c3aad7eMD52TEXTflavio_lara_etal_IOC_2015.PDF.txtflavio_lara_etal_IOC_2015.PDF.txtExtracted 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dc.title.pt_BR.fl_str_mv |
ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells |
title |
ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells |
spellingShingle |
ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells Lara, Flavio Alves Tick Midgut Cells Pesticide Detoxification ATP Binding Cassette Transporter Heme Trifosfato de Adenosina Transportadores de Cassetes de Ligação de ATP Carrapatos Pesticidas Heme |
title_short |
ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells |
title_full |
ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells |
title_fullStr |
ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells |
title_full_unstemmed |
ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells |
title_sort |
ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells |
author |
Lara, Flavio Alves |
author_facet |
Lara, Flavio Alves Pohl, Paula C. Gandara, Ana Caroline Ferreira, Jessica da Silva Silva, Maria Clara Nascimento Bechara, Gervásio Henrique Sorgine, Marcos H. F. Almeida, Igor C. Vaz Junior, Itabajara da Silva Oliveira, Pedro L. |
author_role |
author |
author2 |
Pohl, Paula C. Gandara, Ana Caroline Ferreira, Jessica da Silva Silva, Maria Clara Nascimento Bechara, Gervásio Henrique Sorgine, Marcos H. F. Almeida, Igor C. Vaz Junior, Itabajara da Silva Oliveira, Pedro L. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lara, Flavio Alves Pohl, Paula C. Gandara, Ana Caroline Ferreira, Jessica da Silva Silva, Maria Clara Nascimento Bechara, Gervásio Henrique Sorgine, Marcos H. F. Almeida, Igor C. Vaz Junior, Itabajara da Silva Oliveira, Pedro L. |
dc.subject.en.pt_BR.fl_str_mv |
Tick Midgut Cells Pesticide Detoxification ATP Binding Cassette Transporter Heme |
topic |
Tick Midgut Cells Pesticide Detoxification ATP Binding Cassette Transporter Heme Trifosfato de Adenosina Transportadores de Cassetes de Ligação de ATP Carrapatos Pesticidas Heme |
dc.subject.decs.pt_BR.fl_str_mv |
Trifosfato de Adenosina Transportadores de Cassetes de Ligação de ATP Carrapatos Pesticidas Heme |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2016-03-03T13:21:47Z |
dc.date.available.fl_str_mv |
2016-03-03T13:21:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LARA, Flavio Alves; et al. ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells. Plos One, v.10, n.8, 20p, Aug. 2015. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/12954 |
dc.identifier.issn.none.fl_str_mv |
1932-6203 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0134779 |
identifier_str_mv |
LARA, Flavio Alves; et al. ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells. Plos One, v.10, n.8, 20p, Aug. 2015. 1932-6203 10.1371/journal.pone.0134779 |
url |
https://www.arca.fiocruz.br/handle/icict/12954 |
dc.language.iso.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Public Library of Science |
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Public Library of Science |
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