ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells

Detalhes bibliográficos
Autor(a) principal: Lara, Flavio Alves
Data de Publicação: 2015
Outros Autores: Pohl, Paula C., Gandara, Ana Caroline, Ferreira, Jessica da Silva, Silva, Maria Clara Nascimento, Bechara, Gervásio Henrique, Sorgine, Marcos H. F., Almeida, Igor C., Vaz Junior, Itabajara da Silva, Oliveira, Pedro L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12954
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
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spelling Lara, Flavio AlvesPohl, Paula C.Gandara, Ana CarolineFerreira, Jessica da SilvaSilva, Maria Clara NascimentoBechara, Gervásio HenriqueSorgine, Marcos H. F.Almeida, Igor C.Vaz Junior, Itabajara da SilvaOliveira, Pedro L.2016-03-03T13:21:47Z2016-03-03T13:21:47Z2015LARA, Flavio Alves; et al. ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells. Plos One, v.10, n.8, 20p, Aug. 2015.1932-6203https://www.arca.fiocruz.br/handle/icict/1295410.1371/journal.pone.0134779engPublic Library of ScienceATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Centro de Biotecnologia. Porto Alegre, RS, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Rio de Janeiro, RJ, Brasil.Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinárias. Departamento de Patologia Veterinária. Jaboticabal, SP, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular–INCTEM. Rio de Janeiro, RJ, Brasil.University of Texas at El Paso. The Border Biomedical Research Center. El Paso, Texas, USA.Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Centro de Biotecnologia. Porto Alegre, RS, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular–INCTEM. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Programa de Biologia Molecular e Biotecnologia. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular–INCTEM. Rio de Janeiro, RJ, Brasil.In ticks, the digestion of blood occurs intracellularly and proteolytic digestion of hemoglobin takes place in a dedicated type of lysosome, the digest vesicle, followed by transfer of the heme moiety of hemoglobin to a specialized organelle that accumulates large heme aggregates, called hemosomes. In the present work, we studied the uptake of fluorescent metalloporphyrins, used as heme analogs, and amitraz, one of the most regularly used acaricides to control cattle tick infestations, by Rhipicephalus (Boophilus) microplus midgut cells. Both compounds were taken up by midgut cells in vitro and accumulated inside the hemosomes. Transport of both molecules was sensitive to cyclosporine A (CsA), a wellknown inhibitor of ATP binding cassette (ABC) transporters. Rhodamine 123, a fluorescent probe that is also a recognized ABC substrate, was similarly directed to the hemosome in a CsA-sensitive manner. Using an antibody against conserved domain of PgP-1-type ABC transporter, we were able to immunolocalize PgP-1 in the digest vesicle membranes. Comparison between two R. microplus strains that were resistant and susceptible to amitraz revealed that the resistant strain detoxified both amitraz and Sn-Pp IX more efficiently than the susceptible strain, a process that was also sensitive to CsA. A transcript containing an ABC transporter signature exhibited 2.5-fold increased expression in the amitraz-resistant strain when compared with the susceptible strain. RNAi-induced down-regulation of this ABC transporter led to the accumulation of metalloporphyrin in the digestive vacuole, interrupting heme traffic to the hemosome. This evidence further confirms that this transcript codes for a heme transporter. This is the first report of heme transport in a blood-feeding organism. While the primary physiological function of the hemosome is to detoxify heme and attenuate its toxicity, we suggest that the use of this acaricide detoxification pathway by ticks may represent a new molecular mechanism of resistance to pesticides.Tick Midgut CellsPesticide DetoxificationATP Binding Cassette TransporterHemeTrifosfato de AdenosinaTransportadores de Cassetes de Ligação de ATPCarrapatosPesticidasHemeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12954/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALflavio_lara_etal_IOC_2015.PDFflavio_lara_etal_IOC_2015.PDFapplication/pdf6748083https://www.arca.fiocruz.br/bitstream/icict/12954/2/flavio_lara_etal_IOC_2015.PDF0febe6bad842ab7fb38d1aad3c3aad7eMD52TEXTflavio_lara_etal_IOC_2015.PDF.txtflavio_lara_etal_IOC_2015.PDF.txtExtracted 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dc.title.pt_BR.fl_str_mv ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells
title ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells
spellingShingle ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells
Lara, Flavio Alves
Tick Midgut Cells
Pesticide Detoxification
ATP Binding Cassette Transporter
Heme
Trifosfato de Adenosina
Transportadores de Cassetes de Ligação de ATP
Carrapatos
Pesticidas
Heme
title_short ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells
title_full ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells
title_fullStr ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells
title_full_unstemmed ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells
title_sort ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells
author Lara, Flavio Alves
author_facet Lara, Flavio Alves
Pohl, Paula C.
Gandara, Ana Caroline
Ferreira, Jessica da Silva
Silva, Maria Clara Nascimento
Bechara, Gervásio Henrique
Sorgine, Marcos H. F.
Almeida, Igor C.
Vaz Junior, Itabajara da Silva
Oliveira, Pedro L.
author_role author
author2 Pohl, Paula C.
Gandara, Ana Caroline
Ferreira, Jessica da Silva
Silva, Maria Clara Nascimento
Bechara, Gervásio Henrique
Sorgine, Marcos H. F.
Almeida, Igor C.
Vaz Junior, Itabajara da Silva
Oliveira, Pedro L.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lara, Flavio Alves
Pohl, Paula C.
Gandara, Ana Caroline
Ferreira, Jessica da Silva
Silva, Maria Clara Nascimento
Bechara, Gervásio Henrique
Sorgine, Marcos H. F.
Almeida, Igor C.
Vaz Junior, Itabajara da Silva
Oliveira, Pedro L.
dc.subject.en.pt_BR.fl_str_mv Tick Midgut Cells
Pesticide Detoxification
ATP Binding Cassette Transporter
Heme
topic Tick Midgut Cells
Pesticide Detoxification
ATP Binding Cassette Transporter
Heme
Trifosfato de Adenosina
Transportadores de Cassetes de Ligação de ATP
Carrapatos
Pesticidas
Heme
dc.subject.decs.pt_BR.fl_str_mv Trifosfato de Adenosina
Transportadores de Cassetes de Ligação de ATP
Carrapatos
Pesticidas
Heme
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-03-03T13:21:47Z
dc.date.available.fl_str_mv 2016-03-03T13:21:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LARA, Flavio Alves; et al. ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells. Plos One, v.10, n.8, 20p, Aug. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12954
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0134779
identifier_str_mv LARA, Flavio Alves; et al. ATP Binding Cassette Transporter Mediates Both Heme and Pesticide Detoxification in Tick Midgut Cells. Plos One, v.10, n.8, 20p, Aug. 2015.
1932-6203
10.1371/journal.pone.0134779
url https://www.arca.fiocruz.br/handle/icict/12954
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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