Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/42374 |
Resumo: | Brazilian agencies Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM) and Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). |
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Lima, Emilly J. S. P. deFontes, Sheila S.Nogueira, Mateus LimaSilva, Valdenizia RodriguesSantos, Luciano de S.D'Elia, Gigliola M. A.Dias, Rosane BorgesSales, Caroline Brandi SchlaepferRocha, Clarissa Araújo GurgelSantos, Marcos André VannierSoares, Milena Botelho PereiraCosta, Emmanoel VilaçaSilva, Felipe Moura Araujo daKoolen, Hector Henrique FerreiraBezerra, Daniel Pereira2020-07-24T13:15:18Z2020-07-24T13:15:18Z2020LIMA, Emilly J. S. P. de et al. Essential from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model. Biomedicine & Pharmacotherapy, v. 129, 2020.0753-3322https://www.arca.fiocruz.br/handle/icict/4237410.1016/j.biopha.2020.110402Brazilian agencies Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM) and Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry. Department of Clinical Propaedeutics and Integrated Clinical. Salvador, BA, Brazil.Federal University of Bahia. Institute of Health Sciences. Department of Biomorphology. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Dentistry. Department of Clinical Propaedeutics and Integrated Clinical. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil.Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil.Conobea scoparioides (Cham. & Schltdl.) Benth. (syn. Sphaerotheca scoparioides Cham. & Schldtl.) (Plantaginaceae), popularly known as "pataqueira", "vassourinha-do-brejo" and/or "hierba-de-sapo", is a popular medicinal plant used to treat leishmaniasis, pain and beriberi. In addition, inhibition of cell adhesion, antioxidant, cytotoxic and leishmanicidal activities of compounds or fractions of C. scoparioides have been reported. In the present work, chemical constituents and in vitro and in vivo anti-liver cancer potential of essential oil (EO) from leaves of C. scoparioides were investigated using human hepatocellular carcinoma HepG2 cells as a cell model. EO was obtained by hydrodistillation using a Clevenger-type apparatus and characterized by GC-MS and GC-FID. The in vitro cytotoxic effect was evaluated on three human cancer cell lines (MCF-7, HepG2 and HCT116) and one human non-cancerous cell line (MRC-5) using the Alamar blue assay. Phosphatidylserine externalization and cell cycle distribution were quantified in HepG2 cells by flow cytometry after 48 h incubation. The effectiveness of EO in anti-liver cancer model was studied with HepG2 cells grafted on C.B. 17 SCID mice. The main constituents of EO were thymol methyl ether (62 %), thymol (16 %) and α-phellandrene (14 %). EO displayed an in vitro cytotoxic effect against all human cancer cell lines and caused externalization of phosphatidylserine and DNA fragmentation in HepG2 cells, suggesting induction of apoptotic-like cell death. In vivo tumor mass inhibition of 36.7 and 55.8 % was observed for treatment with EO at doses of 40 and 80 mg/kg, respectively. These results indicate in vitro and in vivo anti-liver cancer potential of EO from leaves of C. scoparioides.engElsevierConobea scoparioidesPlantaginaceaeCâncer fígadoApoptoseHepG2Conobea scoparioidesPlantaginaceaeAnti-liver cancerApoptosisHepG2Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/42374/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLima, Emilly J. S. P. de Essential....pdfLima, Emilly J. S. P. de Essential....pdfapplication/pdf2248368https://www.arca.fiocruz.br/bitstream/icict/42374/2/Lima%2c%20Emilly%20J.%20S.%20P.%20de%20Essential....pdf3f9c3e0f2f30af9da1cb36680a392211MD52TEXTLima, Emilly J. S. P. de Essential....pdf.txtLima, Emilly J. S. 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| dc.title.pt_BR.fl_str_mv |
Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model |
| title |
Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model |
| spellingShingle |
Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model Lima, Emilly J. S. P. de Conobea scoparioides Plantaginaceae Câncer fígado Apoptose HepG2 Conobea scoparioides Plantaginaceae Anti-liver cancer Apoptosis HepG2 |
| title_short |
Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model |
| title_full |
Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model |
| title_fullStr |
Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model |
| title_full_unstemmed |
Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model |
| title_sort |
Essential oil from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model |
| author |
Lima, Emilly J. S. P. de |
| author_facet |
Lima, Emilly J. S. P. de Fontes, Sheila S. Nogueira, Mateus Lima Silva, Valdenizia Rodrigues Santos, Luciano de S. D'Elia, Gigliola M. A. Dias, Rosane Borges Sales, Caroline Brandi Schlaepfer Rocha, Clarissa Araújo Gurgel Santos, Marcos André Vannier Soares, Milena Botelho Pereira Costa, Emmanoel Vilaça Silva, Felipe Moura Araujo da Koolen, Hector Henrique Ferreira Bezerra, Daniel Pereira |
| author_role |
author |
| author2 |
Fontes, Sheila S. Nogueira, Mateus Lima Silva, Valdenizia Rodrigues Santos, Luciano de S. D'Elia, Gigliola M. A. Dias, Rosane Borges Sales, Caroline Brandi Schlaepfer Rocha, Clarissa Araújo Gurgel Santos, Marcos André Vannier Soares, Milena Botelho Pereira Costa, Emmanoel Vilaça Silva, Felipe Moura Araujo da Koolen, Hector Henrique Ferreira Bezerra, Daniel Pereira |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lima, Emilly J. S. P. de Fontes, Sheila S. Nogueira, Mateus Lima Silva, Valdenizia Rodrigues Santos, Luciano de S. D'Elia, Gigliola M. A. Dias, Rosane Borges Sales, Caroline Brandi Schlaepfer Rocha, Clarissa Araújo Gurgel Santos, Marcos André Vannier Soares, Milena Botelho Pereira Costa, Emmanoel Vilaça Silva, Felipe Moura Araujo da Koolen, Hector Henrique Ferreira Bezerra, Daniel Pereira |
| dc.subject.other.pt_BR.fl_str_mv |
Conobea scoparioides Plantaginaceae Câncer fígado Apoptose HepG2 |
| topic |
Conobea scoparioides Plantaginaceae Câncer fígado Apoptose HepG2 Conobea scoparioides Plantaginaceae Anti-liver cancer Apoptosis HepG2 |
| dc.subject.en.pt_BR.fl_str_mv |
Conobea scoparioides Plantaginaceae Anti-liver cancer Apoptosis HepG2 |
| description |
Brazilian agencies Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Amazonas (FAPEAM) and Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). |
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2020 |
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2020-07-24T13:15:18Z |
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2020-07-24T13:15:18Z |
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2020 |
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LIMA, Emilly J. S. P. de et al. Essential from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model. Biomedicine & Pharmacotherapy, v. 129, 2020. |
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https://www.arca.fiocruz.br/handle/icict/42374 |
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0753-3322 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.biopha.2020.110402 |
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LIMA, Emilly J. S. P. de et al. Essential from leaves of Conobea scoparioides (Cham. & Schltdl.) Benth. (Plantaginaceae) causes cell death in HepG2 cells and inhibits tumor development in a xenograft model. Biomedicine & Pharmacotherapy, v. 129, 2020. 0753-3322 10.1016/j.biopha.2020.110402 |
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Elsevier |
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