Vaccine Self-assembling Immune Matrix is a new delivery platform that enhances immune responses to rHBsAg in mice

Detalhes bibliográficos
Autor(a) principal: Grenfell, Rafaella Fortini Queiroz
Data de Publicação: 2015
Outros Autores: Shollenberger, Lisa M., Samli, E. Farah, Harn, Donald A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12657
Resumo: University of Georgia. College of Veterinary Medicine and The Center for Tropical and Emerging Global Diseases. Department of Infectious Diseases. Athens, Georgia, USA/Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Esquistossomose. Belo Horizonte, MG, Brasil
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spelling Grenfell, Rafaella Fortini QueirozShollenberger, Lisa M.Samli, E. FarahHarn, Donald A.2016-01-28T16:49:40Z2016-01-28T16:49:40Z2015GRENFELL, Rafaella Fortini Queiroz e al. Vaccine Self-assembling Immune Matrix is a new delivery platform that enhances immune responses to rHBsAg in mice. Clin Vaccine Immunol., vol. 22, n. 3, p. 336-43, 2015.1556-6811https://www.arca.fiocruz.br/handle/icict/1265710.1128/CVI.00714-14.engAmerican Society for MicrobiologyVaccine Self-assembling Immune Matrix is a new delivery platform that enhances immune responses to rHBsAg in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Georgia. College of Veterinary Medicine and The Center for Tropical and Emerging Global Diseases. Department of Infectious Diseases. Athens, Georgia, USA/Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Esquistossomose. Belo Horizonte, MG, BrasilUniversity of Georgia. College of Veterinary Medicine and The Center for Tropical and Emerging Global Diseases. Department of Infectious Diseases. Athens, Georgia, USAUniversity of Georgia. College of Veterinary Medicine and The Center for Tropical and Emerging Global Diseases. Department of Infectious Diseases. Athens, Georgia, USAUniversity of Georgia. College of Veterinary Medicine and The Center for Tropical and Emerging Global Diseases. Department of Infectious Diseases. Athens, Georgia, USAVaccination remains the most effective public health tool to prevent infectious diseases. Many vaccines are marginally effective and need enhancement for immunocompromised, elderly, and very young populations. To enhance immunogenicity, we exploited the biphasic property of the (RADA)4 synthetic oligopeptide to create VacSIM (vaccine self-assembling immune matrix), a new delivery method. VacSIM solution can easily be mixed with antigens, organisms, and adjuvants for injection. Postinjection, the peptides self-assemble into hydrated nanofiber gel matrices, forming a depot with antigens and adjuvants in the aqueous phase. We believe the depot provides slow release of immunogens, leading to increased activation of antigen-presenting cells that then drive enhanced immunogenicity. Using recombinant hepatitis B virus surface antigen (rHBsAg) as a model immunogen, we compared VacSIM delivery to delivery in alum or complete Freund's adjuvant (CFA). Delivery of the rHBsAg antigen to mice via VacSIM without adjuvant elicited higher specific IgG responses than when rHBsAg was delivered in alum or CFA. Evaluating IgG subtypes showed a mixed Th1/Th2 type response following immunization with VacSIM, which was driven further toward Th1 with addition of CpG as the adjuvant. Increased specific IgG endpoint titers were observed in both C57BL/6 and BALB/c mice, representative of Th1 and Th2 environments, respectively. Restimulation of splenocytes suggests that VacSIM does not cause an immediate proinflammatory response in the host. Overall, these results suggest that VacSIM, as a new delivery method, has the potential to enhance immunogenicity and efficacy of numerous vaccines.Cytokines/immunologyHepatitis B Antibodies/bloodMice, Inbred BALB CVaccines, Synthetic/administration & dosageinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12657/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALVaccine Self-Assembling Immune Matrix Is a New Delivery Platform That Enhances Immune Responses to Recombinant HBsAg in Mice.pdfVaccine Self-Assembling Immune Matrix Is a New Delivery Platform That Enhances Immune Responses to Recombinant HBsAg in 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dc.title.pt_BR.fl_str_mv Vaccine Self-assembling Immune Matrix is a new delivery platform that enhances immune responses to rHBsAg in mice
title Vaccine Self-assembling Immune Matrix is a new delivery platform that enhances immune responses to rHBsAg in mice
spellingShingle Vaccine Self-assembling Immune Matrix is a new delivery platform that enhances immune responses to rHBsAg in mice
Grenfell, Rafaella Fortini Queiroz
Cytokines/immunology
Hepatitis B Antibodies/blood
Mice, Inbred BALB C
Vaccines, Synthetic/administration & dosage
title_short Vaccine Self-assembling Immune Matrix is a new delivery platform that enhances immune responses to rHBsAg in mice
title_full Vaccine Self-assembling Immune Matrix is a new delivery platform that enhances immune responses to rHBsAg in mice
title_fullStr Vaccine Self-assembling Immune Matrix is a new delivery platform that enhances immune responses to rHBsAg in mice
title_full_unstemmed Vaccine Self-assembling Immune Matrix is a new delivery platform that enhances immune responses to rHBsAg in mice
title_sort Vaccine Self-assembling Immune Matrix is a new delivery platform that enhances immune responses to rHBsAg in mice
author Grenfell, Rafaella Fortini Queiroz
author_facet Grenfell, Rafaella Fortini Queiroz
Shollenberger, Lisa M.
Samli, E. Farah
Harn, Donald A.
author_role author
author2 Shollenberger, Lisa M.
Samli, E. Farah
Harn, Donald A.
author2_role author
author
author
dc.contributor.author.fl_str_mv Grenfell, Rafaella Fortini Queiroz
Shollenberger, Lisa M.
Samli, E. Farah
Harn, Donald A.
dc.subject.en.pt_BR.fl_str_mv Cytokines/immunology
Hepatitis B Antibodies/blood
Mice, Inbred BALB C
Vaccines, Synthetic/administration & dosage
topic Cytokines/immunology
Hepatitis B Antibodies/blood
Mice, Inbred BALB C
Vaccines, Synthetic/administration & dosage
description University of Georgia. College of Veterinary Medicine and The Center for Tropical and Emerging Global Diseases. Department of Infectious Diseases. Athens, Georgia, USA/Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratorio de Esquistossomose. Belo Horizonte, MG, Brasil
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-01-28T16:49:40Z
dc.date.available.fl_str_mv 2016-01-28T16:49:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GRENFELL, Rafaella Fortini Queiroz e al. Vaccine Self-assembling Immune Matrix is a new delivery platform that enhances immune responses to rHBsAg in mice. Clin Vaccine Immunol., vol. 22, n. 3, p. 336-43, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12657
dc.identifier.issn.none.fl_str_mv 1556-6811
dc.identifier.doi.none.fl_str_mv 10.1128/CVI.00714-14.
identifier_str_mv GRENFELL, Rafaella Fortini Queiroz e al. Vaccine Self-assembling Immune Matrix is a new delivery platform that enhances immune responses to rHBsAg in mice. Clin Vaccine Immunol., vol. 22, n. 3, p. 336-43, 2015.
1556-6811
10.1128/CVI.00714-14.
url https://www.arca.fiocruz.br/handle/icict/12657
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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