Detection of Trypanosoma cruzi in Blood Specimens of Chronic Chagasic Patients by Polymerase Chain Reaction Amplification of Kinetoplast Minicircle DNA: Comparison with Serology and Xenodiagnosis

Detalhes bibliográficos
Autor(a) principal: Avila, Herbert A.
Data de Publicação: 1993
Outros Autores: Pereira, Jose Borges, Thiemann, Otavio, Paiva, Eugenia de, Degrave, Wim, Morel, Carlos Medicis, Simpson, Larry
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14660
Resumo: University of California. Department of Biology. Los Angeles, CA, USA.
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spelling Avila, Herbert A.Pereira, Jose BorgesThiemann, OtavioPaiva, Eugenia deDegrave, WimMorel, Carlos MedicisSimpson, Larry2016-06-29T14:58:50Z2016-07-07T12:05:14Z2016-06-29T14:58:50Z2016-07-07T12:05:14Z1993AVILA, Herbert A. et al. Detection of Trypanosoma cruzi in blood specimens of chronic chagasic patients by polymerase chain reaction amplification of kinetoplast minicircle DNA: comparison with serology and xenodiagnosis. Journal of Clinical Microbiology, v. 31, n. 9, p. 2421-2426, 1993.0095-1137https://www.arca.fiocruz.br/handle/icict/146601098-660XengAmerican Society for MicrobiologyDetection of Trypanosoma cruzi in Blood Specimens of Chronic Chagasic Patients by Polymerase Chain Reaction Amplification of Kinetoplast Minicircle DNA: Comparison with Serology and Xenodiagnosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of California. Department of Biology. Los Angeles, CA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical Rio de Janeiro, RJ, Brasil.University of California. Department of Biology. Los Angeles, CA, USA.University of California. Department of Biology. Los Angeles, CA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.University of California. Department of Biology. Los Angeles, CA, USA / University of California. Molecular Biology Institute. Los Angeles, CA, USA / University of California. Howard Hughes Medical Institute. Los Angeles, CA, USA.A panel of 114 blood samples from chronic chagasic patients and nonchagasic patients was screened for Trypanosoma cruzi by xenodiagnostic, serologic, and polymerase chain reaction (PCR) amplification tests. Blood samples were preserved in a guanidine-EDTA buffer, and total blood DNA was isolated after chemical nuclease cleavage with 1,10-phenanthroline-copper ion and used as a template for PCR amplification of the conserved and variable regions of T. cruzi minicircle molecules. The PCR products were screened by Southern blot hybridization with a digoxigenin-labeled oligonucleotide probe specific for the conserved region of the minicircle. The method showed a sensitivity of 100%o compared with the serologic test. In addition, all of the serology-positive, xenodiagnosis-negative samples were positive by PCR. This demonstrates that PCR amplification of T. cruzi kinetoplast minicircle DNA could replace xenodiagnosis for evaluation of parasitemia in chronic chagasic patients and could serve as a complement for serologic testing in the screening of blood bank donors.Trypanosoma cruziChronic Chagasic PatientsPolymerase Chain ReactionKinetoplast Minicircle DNASerologyXenodiagnosisTrypanosoma cruziXenodiagnósticoDoença de ChagasReação em Cadeia da PolimeraseSorologiaDNA de Cinetoplastoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTcarlos17_morel_etal_IOC_1993.pdf.txtcarlos17_morel_etal_IOC_1993.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Detection of Trypanosoma cruzi in Blood Specimens of Chronic Chagasic Patients by Polymerase Chain Reaction Amplification of Kinetoplast Minicircle DNA: Comparison with Serology and Xenodiagnosis
title Detection of Trypanosoma cruzi in Blood Specimens of Chronic Chagasic Patients by Polymerase Chain Reaction Amplification of Kinetoplast Minicircle DNA: Comparison with Serology and Xenodiagnosis
spellingShingle Detection of Trypanosoma cruzi in Blood Specimens of Chronic Chagasic Patients by Polymerase Chain Reaction Amplification of Kinetoplast Minicircle DNA: Comparison with Serology and Xenodiagnosis
Avila, Herbert A.
Trypanosoma cruzi
Chronic Chagasic Patients
Polymerase Chain Reaction
Kinetoplast Minicircle DNA
Serology
Xenodiagnosis
Trypanosoma cruzi
Xenodiagnóstico
Doença de Chagas
Reação em Cadeia da Polimerase
Sorologia
DNA de Cinetoplasto
title_short Detection of Trypanosoma cruzi in Blood Specimens of Chronic Chagasic Patients by Polymerase Chain Reaction Amplification of Kinetoplast Minicircle DNA: Comparison with Serology and Xenodiagnosis
title_full Detection of Trypanosoma cruzi in Blood Specimens of Chronic Chagasic Patients by Polymerase Chain Reaction Amplification of Kinetoplast Minicircle DNA: Comparison with Serology and Xenodiagnosis
title_fullStr Detection of Trypanosoma cruzi in Blood Specimens of Chronic Chagasic Patients by Polymerase Chain Reaction Amplification of Kinetoplast Minicircle DNA: Comparison with Serology and Xenodiagnosis
title_full_unstemmed Detection of Trypanosoma cruzi in Blood Specimens of Chronic Chagasic Patients by Polymerase Chain Reaction Amplification of Kinetoplast Minicircle DNA: Comparison with Serology and Xenodiagnosis
title_sort Detection of Trypanosoma cruzi in Blood Specimens of Chronic Chagasic Patients by Polymerase Chain Reaction Amplification of Kinetoplast Minicircle DNA: Comparison with Serology and Xenodiagnosis
author Avila, Herbert A.
author_facet Avila, Herbert A.
Pereira, Jose Borges
Thiemann, Otavio
Paiva, Eugenia de
Degrave, Wim
Morel, Carlos Medicis
Simpson, Larry
author_role author
author2 Pereira, Jose Borges
Thiemann, Otavio
Paiva, Eugenia de
Degrave, Wim
Morel, Carlos Medicis
Simpson, Larry
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Avila, Herbert A.
Pereira, Jose Borges
Thiemann, Otavio
Paiva, Eugenia de
Degrave, Wim
Morel, Carlos Medicis
Simpson, Larry
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Chronic Chagasic Patients
Polymerase Chain Reaction
Kinetoplast Minicircle DNA
Serology
Xenodiagnosis
topic Trypanosoma cruzi
Chronic Chagasic Patients
Polymerase Chain Reaction
Kinetoplast Minicircle DNA
Serology
Xenodiagnosis
Trypanosoma cruzi
Xenodiagnóstico
Doença de Chagas
Reação em Cadeia da Polimerase
Sorologia
DNA de Cinetoplasto
dc.subject.decs.pt_BR.fl_str_mv Trypanosoma cruzi
Xenodiagnóstico
Doença de Chagas
Reação em Cadeia da Polimerase
Sorologia
DNA de Cinetoplasto
description University of California. Department of Biology. Los Angeles, CA, USA.
publishDate 1993
dc.date.issued.fl_str_mv 1993
dc.date.accessioned.fl_str_mv 2016-06-29T14:58:50Z
2016-07-07T12:05:14Z
dc.date.available.fl_str_mv 2016-06-29T14:58:50Z
2016-07-07T12:05:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv AVILA, Herbert A. et al. Detection of Trypanosoma cruzi in blood specimens of chronic chagasic patients by polymerase chain reaction amplification of kinetoplast minicircle DNA: comparison with serology and xenodiagnosis. Journal of Clinical Microbiology, v. 31, n. 9, p. 2421-2426, 1993.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14660
dc.identifier.issn.pt_BR.fl_str_mv 0095-1137
dc.identifier.eissn.pt_BR.fl_str_mv 1098-660X
identifier_str_mv AVILA, Herbert A. et al. Detection of Trypanosoma cruzi in blood specimens of chronic chagasic patients by polymerase chain reaction amplification of kinetoplast minicircle DNA: comparison with serology and xenodiagnosis. Journal of Clinical Microbiology, v. 31, n. 9, p. 2421-2426, 1993.
0095-1137
1098-660X
url https://www.arca.fiocruz.br/handle/icict/14660
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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collection Repositório Institucional da FIOCRUZ (ARCA)
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