Recirculation of Giardia lamblia Assemblage A After Metronidazole Treatment in an Area With Assemblages A, B, and E Sympatric Circulation

Detalhes bibliográficos
Autor(a) principal: Fantinatti, Maria
Data de Publicação: 2020
Outros Autores: Oliveira, Luiz Antonio Pimentel Lopes, Cascais-Figueredo, Tiara, Austriaco-Teixeira, Phelipe, Verissimo, Erika, Bello, Alexandre Ribeiro, Cruz, Alda Maria da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/45720
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.
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spelling Fantinatti, MariaOliveira, Luiz Antonio Pimentel LopesCascais-Figueredo, TiaraAustriaco-Teixeira, PhelipeVerissimo, ErikaBello, Alexandre RibeiroCruz, Alda Maria da2021-01-16T13:05:31Z2021-01-16T13:05:31Z2020FANTINATTI, Maria et al. Recirculation of Giardia lamblia Assemblage A After Metronidazole Treatment in an Area With Assemblages A, B, and E Sympatric Circulation. Frontiers in Immunology, v. 11, Article 571104, p. 1-9, Oct. 2020.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/4572010.3389/fmicb.2020.571104engFrontiers MediaGiardia lambliaReinfecçãoMetronidazolResistênciaPersistência do parasitaGiardia lambliaAssemblageMetronidazoleReinfectionParasite persistenceResistanceRecirculation of Giardia lamblia Assemblage A After Metronidazole Treatment in an Area With Assemblages A, B, and E Sympatric Circulationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro, Rio de Janeiro. Faculdade de Ciências Médicas. Disciplina de Parasitologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro, Rio de Janeiro. Faculdade de Ciências Médicas. Disciplina de Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro, Rio de Janeiro. Faculdade de Ciências Médicas. Disciplina de Parasitologia. Rio de Janeiro, RJ, Brasil.Giardia lamblia is an intestinal protozoan subdivided into eight assemblages, labeled alphabetically from A to H. Assemblages A, B, and E infect humans and can have a sympatric circulation. We investigated the assemblage recirculation in children living within a high prevalence area of Giardia infection. One hundred and ninety-four children were evaluated and 85 tested positive for Giardia by PCR. These infected individuals were recruited, treated with metronidazole and then reexamined for infections at 20 and 40 days after treatment that included PCR and the genotyping was performed by sequencing beta-giardin and glutamate dehydrogenase gene targets. Giardia assemblages A (n = 43), B (n = 21), E (n = 17), and A/E (n = 4) were identified in infected children. Assemblage A was found in all reoccurrences of infection, including four that had been infected by assemblages B and E. Since both persistence and reinfection could account for the results, the level of nucleotide homology was determined before and after treatment. Most suggested that reinfections were by the same strain, but four presented a distinct genotypic profile. The results suggest that the differences in the genotypic profiles were due to reinfections, which appear to occur with frequency in high Giardia burden areas and soon after the end of therapy. It is not yet possible to define whether the recurrent cases were related to parasite resistance. However, the evidence of rapid reinfections and ready availability of treatment raises the potential for creating resistant strains. This highlights the need to address how Giardia burden is maintained within high prevalence areas.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45720/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMariaFantininati_AldaMariaCruz_etal_IOC_2020.pdfMariaFantininati_AldaMariaCruz_etal_IOC_2020.pdfapplication/pdf1137341https://www.arca.fiocruz.br/bitstream/icict/45720/2/MariaFantininati_AldaMariaCruz_etal_IOC_2020.pdf11c626ab08862ab4360d18b3ff66e9c5MD52TEXTMariaFantininati_AldaMariaCruz_etal_IOC_2020.pdf.txtMariaFantininati_AldaMariaCruz_etal_IOC_2020.pdf.txtExtracted texttext/plain40489https://www.arca.fiocruz.br/bitstream/icict/45720/3/MariaFantininati_AldaMariaCruz_etal_IOC_2020.pdf.txtc74cc31b4c2fe4a6b5ffc17c067124beMD53icict/457202021-01-18 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dc.title.pt_BR.fl_str_mv Recirculation of Giardia lamblia Assemblage A After Metronidazole Treatment in an Area With Assemblages A, B, and E Sympatric Circulation
title Recirculation of Giardia lamblia Assemblage A After Metronidazole Treatment in an Area With Assemblages A, B, and E Sympatric Circulation
spellingShingle Recirculation of Giardia lamblia Assemblage A After Metronidazole Treatment in an Area With Assemblages A, B, and E Sympatric Circulation
Fantinatti, Maria
Giardia lamblia
Reinfecção
Metronidazol
Resistência
Persistência do parasita
Giardia lamblia
Assemblage
Metronidazole
Reinfection
Parasite persistence
Resistance
title_short Recirculation of Giardia lamblia Assemblage A After Metronidazole Treatment in an Area With Assemblages A, B, and E Sympatric Circulation
title_full Recirculation of Giardia lamblia Assemblage A After Metronidazole Treatment in an Area With Assemblages A, B, and E Sympatric Circulation
title_fullStr Recirculation of Giardia lamblia Assemblage A After Metronidazole Treatment in an Area With Assemblages A, B, and E Sympatric Circulation
title_full_unstemmed Recirculation of Giardia lamblia Assemblage A After Metronidazole Treatment in an Area With Assemblages A, B, and E Sympatric Circulation
title_sort Recirculation of Giardia lamblia Assemblage A After Metronidazole Treatment in an Area With Assemblages A, B, and E Sympatric Circulation
author Fantinatti, Maria
author_facet Fantinatti, Maria
Oliveira, Luiz Antonio Pimentel Lopes
Cascais-Figueredo, Tiara
Austriaco-Teixeira, Phelipe
Verissimo, Erika
Bello, Alexandre Ribeiro
Cruz, Alda Maria da
author_role author
author2 Oliveira, Luiz Antonio Pimentel Lopes
Cascais-Figueredo, Tiara
Austriaco-Teixeira, Phelipe
Verissimo, Erika
Bello, Alexandre Ribeiro
Cruz, Alda Maria da
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fantinatti, Maria
Oliveira, Luiz Antonio Pimentel Lopes
Cascais-Figueredo, Tiara
Austriaco-Teixeira, Phelipe
Verissimo, Erika
Bello, Alexandre Ribeiro
Cruz, Alda Maria da
dc.subject.other.pt_BR.fl_str_mv Giardia lamblia
Reinfecção
Metronidazol
Resistência
Persistência do parasita
topic Giardia lamblia
Reinfecção
Metronidazol
Resistência
Persistência do parasita
Giardia lamblia
Assemblage
Metronidazole
Reinfection
Parasite persistence
Resistance
dc.subject.en.pt_BR.fl_str_mv Giardia lamblia
Assemblage
Metronidazole
Reinfection
Parasite persistence
Resistance
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-01-16T13:05:31Z
dc.date.available.fl_str_mv 2021-01-16T13:05:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FANTINATTI, Maria et al. Recirculation of Giardia lamblia Assemblage A After Metronidazole Treatment in an Area With Assemblages A, B, and E Sympatric Circulation. Frontiers in Immunology, v. 11, Article 571104, p. 1-9, Oct. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/45720
dc.identifier.issn.pt_BR.fl_str_mv 1664-302X
dc.identifier.doi.none.fl_str_mv 10.3389/fmicb.2020.571104
identifier_str_mv FANTINATTI, Maria et al. Recirculation of Giardia lamblia Assemblage A After Metronidazole Treatment in an Area With Assemblages A, B, and E Sympatric Circulation. Frontiers in Immunology, v. 11, Article 571104, p. 1-9, Oct. 2020.
1664-302X
10.3389/fmicb.2020.571104
url https://www.arca.fiocruz.br/handle/icict/45720
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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