Effects of metronidazole analogues on Giardia lamblia: experimental infection and cell organization
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1016/j.diagmicrobio.2012.11.001 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/20773 |
Resumo: | Fundação de Amparo à Pesquisa do Estado de Minas–FAPEMIG (grant number APQ 01766–10), FAPESB, PP-SUS, CNPq, and FIOCRUZ |
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Busatti, Haendel Gonçalves Nogueira OliveiraAlves, Ricardo JoséAnjos, Karla Graziela Santana dosGil, Frederico FerreiraCury, Marcia CristinaSantos, Marcos André Vannier dosGomes, Maria Aparecida2017-08-25T17:49:57Z2017-08-25T17:49:57Z2013BUSATTI, H. G. N. O. et al. Effects of metronidazole analogues on Giardia lamblia: experimental infection and cell organization. Diagnostic Microbiology and Infectious Disease, v. 75, p. 160-164, 2013.0732-8893https://www.arca.fiocruz.br/handle/icict/2077310.1016/j.diagmicrobio.2012.11.001Fundação de Amparo à Pesquisa do Estado de Minas–FAPEMIG (grant number APQ 01766–10), FAPESB, PP-SUS, CNPq, and FIOCRUZUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departament de Parasitologia. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento Produtos Farmacêuticos. Belo Horizonte, MG, Brazil / Universidade de Itaúna. Faculdade de Farmácia.Departamento de Parasitologia. Itaúna, MG, Brazil.Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento Produtos Farmacêuticos. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departament de Parasitologia. Belo Horizonte, MG, Brazil.Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Laboratório de Parasitologia. Uberlânida, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departament de Parasitologia. Belo Horizonte, MG, Brazil.The chemotherapeutic agents used for the treatment of giardiasis are often associated with adverse side effects and are refractory cases, due to the development of resistant parasites. Therefore the search for new drugs is required. We have previously reported the giardicidal effects of metronidazole (MTZ) and its analogues (MTZ-Ms, MTZ-Br, MTZ-N(3), and MTZ-I) on the trophozoites of Giardia lamblia. Now we evaluated the activity of some giardicidal MTZ analogues in experimental infections in gerbils and its effects on the morphology and ultrastructural organization of Giardia. The giardicidal activity in experimental infections showed ED(50) values significantly lower for MTZ-I and MTZ-Br when compared to MTZ. Transmission electron microscopy was employed to approach the mechanism(s) of action of MTZ analogues upon the protozoan. MTZ analogues were more active than MTZ in changing significantly the morphology and ultrastructure of the parasite. The analogues affected parasite cell vesicle trafficking, autophagy, and triggered differentiation into cysts. These results coupled with the excellent giardicidal activity and lower toxicity demonstrate that these nitroimidazole derivates may be important therapeutic alternatives for combating giardiasis. In addition, our results suggest a therapeutic advantage in obtaining synthetic metronidazole analogues for screening of activities against other infectious agents.engElsevierGiardia LambliaMetais análogos de metronidazolCarga parasitáriaMicroscopia eletrônicaGiardicidal activityMetronidazole analoguesParasite loadElectron microscopyEffects of metronidazole analogues on Giardia lamblia: experimental infection and cell organizationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20773/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALBusatti HGNO Effects of metronidazole....pdfBusatti HGNO Effects of 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| dc.title.pt_BR.fl_str_mv |
Effects of metronidazole analogues on Giardia lamblia: experimental infection and cell organization |
| title |
Effects of metronidazole analogues on Giardia lamblia: experimental infection and cell organization |
| spellingShingle |
Effects of metronidazole analogues on Giardia lamblia: experimental infection and cell organization Busatti, Haendel Gonçalves Nogueira Oliveira Giardia Lamblia Metais análogos de metronidazol Carga parasitária Microscopia eletrônica Giardicidal activity Metronidazole analogues Parasite load Electron microscopy |
| title_short |
Effects of metronidazole analogues on Giardia lamblia: experimental infection and cell organization |
| title_full |
Effects of metronidazole analogues on Giardia lamblia: experimental infection and cell organization |
| title_fullStr |
Effects of metronidazole analogues on Giardia lamblia: experimental infection and cell organization |
| title_full_unstemmed |
Effects of metronidazole analogues on Giardia lamblia: experimental infection and cell organization |
| title_sort |
Effects of metronidazole analogues on Giardia lamblia: experimental infection and cell organization |
| author |
Busatti, Haendel Gonçalves Nogueira Oliveira |
| author_facet |
Busatti, Haendel Gonçalves Nogueira Oliveira Alves, Ricardo José Anjos, Karla Graziela Santana dos Gil, Frederico Ferreira Cury, Marcia Cristina Santos, Marcos André Vannier dos Gomes, Maria Aparecida |
| author_role |
author |
| author2 |
Alves, Ricardo José Anjos, Karla Graziela Santana dos Gil, Frederico Ferreira Cury, Marcia Cristina Santos, Marcos André Vannier dos Gomes, Maria Aparecida |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Busatti, Haendel Gonçalves Nogueira Oliveira Alves, Ricardo José Anjos, Karla Graziela Santana dos Gil, Frederico Ferreira Cury, Marcia Cristina Santos, Marcos André Vannier dos Gomes, Maria Aparecida |
| dc.subject.other.pt_BR.fl_str_mv |
Giardia Lamblia Metais análogos de metronidazol Carga parasitária Microscopia eletrônica |
| topic |
Giardia Lamblia Metais análogos de metronidazol Carga parasitária Microscopia eletrônica Giardicidal activity Metronidazole analogues Parasite load Electron microscopy |
| dc.subject.en.pt_BR.fl_str_mv |
Giardicidal activity Metronidazole analogues Parasite load Electron microscopy |
| description |
Fundação de Amparo à Pesquisa do Estado de Minas–FAPEMIG (grant number APQ 01766–10), FAPESB, PP-SUS, CNPq, and FIOCRUZ |
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2013 |
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2013 |
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2017-08-25T17:49:57Z |
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2017-08-25T17:49:57Z |
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BUSATTI, H. G. N. O. et al. Effects of metronidazole analogues on Giardia lamblia: experimental infection and cell organization. Diagnostic Microbiology and Infectious Disease, v. 75, p. 160-164, 2013. |
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https://www.arca.fiocruz.br/handle/icict/20773 |
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0732-8893 |
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10.1016/j.diagmicrobio.2012.11.001 |
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BUSATTI, H. G. N. O. et al. Effects of metronidazole analogues on Giardia lamblia: experimental infection and cell organization. Diagnostic Microbiology and Infectious Disease, v. 75, p. 160-164, 2013. 0732-8893 10.1016/j.diagmicrobio.2012.11.001 |
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