New multilocus genotypes of Giardia lamblia human isolates

Detalhes bibliográficos
Autor(a) principal: Faria, C. P.
Data de Publicação: 2017
Outros Autores: Zanini, G. M., Dias, G. S., Silva, S. da, Sousa, M. D. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/34437
Resumo: 2020-07-26
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spelling Faria, C. P.Zanini, G. M.Dias, G. S.Silva, S. daSousa, M. D. C.2019-07-26T12:52:57Z2019-07-26T12:52:57Z2017FARIA, Clarissa Perez et al. New multilocus genotypes of Giardia lamblia human isolates. Infection, Genetics and Evolution, v. 54 p. 128-137, 2017.1567-1348https://www.arca.fiocruz.br/handle/icict/3443710.1016/j.meegid.2017.06.0281567-7257engElsevierNew multilocus genotypes of Giardia lamblia human isolatesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-07-26University of Coimbra. Center for Neurosciences and Cell Biology. Coimbra, Portugal / University of Coimbra. Faculty of Pharmacy. Coimbra, Portugal / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Parasitologia. Rio de Janeiro, RJ, Brasil.University of Coimbra. Center for Neurosciences and Cell Biology. Coimbra, Portugal / University of Coimbra. Faculty of Pharmacy. Coimbra, Portugal.Giardia lamblia is considered a species complex, whose members show little differences in their morphology, but have remarkable genetic variability. The aim of this study was to identify inter-and intra-assemblage genetic variation in G. lamblia among patients in Rio de Janeiro. The parasitological study was performed on faeces, and DNA was extracted from the samples which tested positive for G. lamblia. The genetic assemblages and subtypes were determined via multilocus sequence typing (MLST) using β-giardin, triose phosphate isomerase and glutamate dehydrogenase gene loci. Fourteen assemblage A samples were successfully genotyped at the three MLST loci(bg/tpi/gdh). Two previously identified multilocus genotypes were found (AII-1 and AII-4), and two novel multilocus genotypes are proposed (AII-8, profile A2/A2/A4; AII-9, profile A3/A2/A2). Sequence analysis showed that assemblage B isolates have a higher nucleotide variation than those from assemblage A. Novel assemblage B sequences are described and most (66.7%) have heterogeneous nucleotides, which prevent the definition of multilocus genotypes. This is the first time that MLST has been used to characterise G. lamblia isolates inhuman clinical samples from Rio de Janeiro. In addition, MLST has enabled the detection of novel subtypes in both assemblages and the description of two novel multilocus genotypes in assemblage A. This study provides new insights into the genetic diversity of assemblage A and shows that MLST should be used to characterise G. lamblia both in Brazil and globally.Giardia lambliaAssemblageMultilocus genotypingDNA sequencingβ-giardinGlutamate dehydrogenaseTriose phosphate isomeraseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/34437/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Faria_Clarissa_etal_INI_2017.pdfve_Faria_Clarissa_etal_INI_2017.pdfapplication/pdf406157https://www.arca.fiocruz.br/bitstream/icict/34437/2/ve_Faria_Clarissa_etal_INI_2017.pdf76a32f05c79f85b5d9da59585639603fMD52TEXTve_Faria_Clarissa_etal_INI_2017.pdf.txtve_Faria_Clarissa_etal_INI_2017.pdf.txtExtracted texttext/plain54910https://www.arca.fiocruz.br/bitstream/icict/34437/3/ve_Faria_Clarissa_etal_INI_2017.pdf.txt701d00f97ba523e2329ed9d7d64f8b41MD53icict/344372021-03-24 16:32:34.781oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:34Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv New multilocus genotypes of Giardia lamblia human isolates
title New multilocus genotypes of Giardia lamblia human isolates
spellingShingle New multilocus genotypes of Giardia lamblia human isolates
Faria, C. P.
Giardia lamblia
Assemblage
Multilocus genotyping
DNA sequencing
β-giardin
Glutamate dehydrogenase
Triose phosphate isomerase
title_short New multilocus genotypes of Giardia lamblia human isolates
title_full New multilocus genotypes of Giardia lamblia human isolates
title_fullStr New multilocus genotypes of Giardia lamblia human isolates
title_full_unstemmed New multilocus genotypes of Giardia lamblia human isolates
title_sort New multilocus genotypes of Giardia lamblia human isolates
author Faria, C. P.
author_facet Faria, C. P.
Zanini, G. M.
Dias, G. S.
Silva, S. da
Sousa, M. D. C.
author_role author
author2 Zanini, G. M.
Dias, G. S.
Silva, S. da
Sousa, M. D. C.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Faria, C. P.
Zanini, G. M.
Dias, G. S.
Silva, S. da
Sousa, M. D. C.
dc.subject.en.pt_BR.fl_str_mv Giardia lamblia
Assemblage
Multilocus genotyping
DNA sequencing
β-giardin
Glutamate dehydrogenase
Triose phosphate isomerase
topic Giardia lamblia
Assemblage
Multilocus genotyping
DNA sequencing
β-giardin
Glutamate dehydrogenase
Triose phosphate isomerase
description 2020-07-26
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2019-07-26T12:52:57Z
dc.date.available.fl_str_mv 2019-07-26T12:52:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FARIA, Clarissa Perez et al. New multilocus genotypes of Giardia lamblia human isolates. Infection, Genetics and Evolution, v. 54 p. 128-137, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/34437
dc.identifier.issn.pt_BR.fl_str_mv 1567-1348
dc.identifier.doi.none.fl_str_mv 10.1016/j.meegid.2017.06.028
dc.identifier.eissn.none.fl_str_mv 1567-7257
identifier_str_mv FARIA, Clarissa Perez et al. New multilocus genotypes of Giardia lamblia human isolates. Infection, Genetics and Evolution, v. 54 p. 128-137, 2017.
1567-1348
10.1016/j.meegid.2017.06.028
1567-7257
url https://www.arca.fiocruz.br/handle/icict/34437
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/34437/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/34437/2/ve_Faria_Clarissa_etal_INI_2017.pdf
https://www.arca.fiocruz.br/bitstream/icict/34437/3/ve_Faria_Clarissa_etal_INI_2017.pdf.txt
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