New multilocus genotypes of Giardia lamblia human isolates
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/34437 |
Resumo: | 2020-07-26 |
id |
CRUZ_7a3460e569ea62ed1cd8d2d49da72fdf |
---|---|
oai_identifier_str |
oai:www.arca.fiocruz.br:icict/34437 |
network_acronym_str |
CRUZ |
network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
repository_id_str |
2135 |
spelling |
Faria, C. P.Zanini, G. M.Dias, G. S.Silva, S. daSousa, M. D. C.2019-07-26T12:52:57Z2019-07-26T12:52:57Z2017FARIA, Clarissa Perez et al. New multilocus genotypes of Giardia lamblia human isolates. Infection, Genetics and Evolution, v. 54 p. 128-137, 2017.1567-1348https://www.arca.fiocruz.br/handle/icict/3443710.1016/j.meegid.2017.06.0281567-7257engElsevierNew multilocus genotypes of Giardia lamblia human isolatesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-07-26University of Coimbra. Center for Neurosciences and Cell Biology. Coimbra, Portugal / University of Coimbra. Faculty of Pharmacy. Coimbra, Portugal / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Parasitologia. Rio de Janeiro, RJ, Brasil.University of Coimbra. Center for Neurosciences and Cell Biology. Coimbra, Portugal / University of Coimbra. Faculty of Pharmacy. Coimbra, Portugal.Giardia lamblia is considered a species complex, whose members show little differences in their morphology, but have remarkable genetic variability. The aim of this study was to identify inter-and intra-assemblage genetic variation in G. lamblia among patients in Rio de Janeiro. The parasitological study was performed on faeces, and DNA was extracted from the samples which tested positive for G. lamblia. The genetic assemblages and subtypes were determined via multilocus sequence typing (MLST) using β-giardin, triose phosphate isomerase and glutamate dehydrogenase gene loci. Fourteen assemblage A samples were successfully genotyped at the three MLST loci(bg/tpi/gdh). Two previously identified multilocus genotypes were found (AII-1 and AII-4), and two novel multilocus genotypes are proposed (AII-8, profile A2/A2/A4; AII-9, profile A3/A2/A2). Sequence analysis showed that assemblage B isolates have a higher nucleotide variation than those from assemblage A. Novel assemblage B sequences are described and most (66.7%) have heterogeneous nucleotides, which prevent the definition of multilocus genotypes. This is the first time that MLST has been used to characterise G. lamblia isolates inhuman clinical samples from Rio de Janeiro. In addition, MLST has enabled the detection of novel subtypes in both assemblages and the description of two novel multilocus genotypes in assemblage A. This study provides new insights into the genetic diversity of assemblage A and shows that MLST should be used to characterise G. lamblia both in Brazil and globally.Giardia lambliaAssemblageMultilocus genotypingDNA sequencingβ-giardinGlutamate dehydrogenaseTriose phosphate isomeraseinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/34437/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Faria_Clarissa_etal_INI_2017.pdfve_Faria_Clarissa_etal_INI_2017.pdfapplication/pdf406157https://www.arca.fiocruz.br/bitstream/icict/34437/2/ve_Faria_Clarissa_etal_INI_2017.pdf76a32f05c79f85b5d9da59585639603fMD52TEXTve_Faria_Clarissa_etal_INI_2017.pdf.txtve_Faria_Clarissa_etal_INI_2017.pdf.txtExtracted texttext/plain54910https://www.arca.fiocruz.br/bitstream/icict/34437/3/ve_Faria_Clarissa_etal_INI_2017.pdf.txt701d00f97ba523e2329ed9d7d64f8b41MD53icict/344372021-03-24 16:32:34.781oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:34Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
New multilocus genotypes of Giardia lamblia human isolates |
title |
New multilocus genotypes of Giardia lamblia human isolates |
spellingShingle |
New multilocus genotypes of Giardia lamblia human isolates Faria, C. P. Giardia lamblia Assemblage Multilocus genotyping DNA sequencing β-giardin Glutamate dehydrogenase Triose phosphate isomerase |
title_short |
New multilocus genotypes of Giardia lamblia human isolates |
title_full |
New multilocus genotypes of Giardia lamblia human isolates |
title_fullStr |
New multilocus genotypes of Giardia lamblia human isolates |
title_full_unstemmed |
New multilocus genotypes of Giardia lamblia human isolates |
title_sort |
New multilocus genotypes of Giardia lamblia human isolates |
author |
Faria, C. P. |
author_facet |
Faria, C. P. Zanini, G. M. Dias, G. S. Silva, S. da Sousa, M. D. C. |
author_role |
author |
author2 |
Zanini, G. M. Dias, G. S. Silva, S. da Sousa, M. D. C. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Faria, C. P. Zanini, G. M. Dias, G. S. Silva, S. da Sousa, M. D. C. |
dc.subject.en.pt_BR.fl_str_mv |
Giardia lamblia Assemblage Multilocus genotyping DNA sequencing β-giardin Glutamate dehydrogenase Triose phosphate isomerase |
topic |
Giardia lamblia Assemblage Multilocus genotyping DNA sequencing β-giardin Glutamate dehydrogenase Triose phosphate isomerase |
description |
2020-07-26 |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2019-07-26T12:52:57Z |
dc.date.available.fl_str_mv |
2019-07-26T12:52:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FARIA, Clarissa Perez et al. New multilocus genotypes of Giardia lamblia human isolates. Infection, Genetics and Evolution, v. 54 p. 128-137, 2017. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/34437 |
dc.identifier.issn.pt_BR.fl_str_mv |
1567-1348 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.meegid.2017.06.028 |
dc.identifier.eissn.none.fl_str_mv |
1567-7257 |
identifier_str_mv |
FARIA, Clarissa Perez et al. New multilocus genotypes of Giardia lamblia human isolates. Infection, Genetics and Evolution, v. 54 p. 128-137, 2017. 1567-1348 10.1016/j.meegid.2017.06.028 1567-7257 |
url |
https://www.arca.fiocruz.br/handle/icict/34437 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
collection |
Repositório Institucional da FIOCRUZ (ARCA) |
bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/34437/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/34437/2/ve_Faria_Clarissa_etal_INI_2017.pdf https://www.arca.fiocruz.br/bitstream/icict/34437/3/ve_Faria_Clarissa_etal_INI_2017.pdf.txt |
bitstream.checksum.fl_str_mv |
8a4605be74aa9ea9d79846c1fba20a33 76a32f05c79f85b5d9da59585639603f 701d00f97ba523e2329ed9d7d64f8b41 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
_version_ |
1798324987100135424 |