IFN-gamma production by CD8+ T cells depends on NFAT1 transcription factor and regulates Th differentiation

Detalhes bibliográficos
Autor(a) principal: Teixeira, Leonardo K.
Data de Publicação: 2005
Outros Autores: Fonseca, Bruna P. F., Abreu, Adriana Vieira de, Barboza, Bianca A., Robbs, Bruno K., Bozza, Patrícia T., Viola, João P. B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/40903
Resumo: Instituto Nacional de Câncer. Divisão de Biologia Celular. Rio de Janeiro, RJ, Brasil.
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spelling Teixeira, Leonardo K.Fonseca, Bruna P. F.Abreu, Adriana Vieira deBarboza, Bianca A.Robbs, Bruno K.Bozza, Patrícia T.Viola, João P. B.2020-04-21T14:09:41Z2020-04-21T14:09:41Z2005TEIXEIRA, Leonardo K. et al. IFN- Production by CD8 T Cells Depends on NFAT1 Transcription Factor and Regulates Th Differentiation. Journal of Immunology, v. 175, p. 5931-5939, 2005.0022-1767https://www.arca.fiocruz.br/handle/icict/4090310.4049/jimmunol.175.9.59311550-6606engAmerican Association of ImmunologistsCD8 T CellsIFN-yLInfócitos TCD8 T CellsIFN-y ProductionT lymphocytesIFN-gamma production by CD8+ T cells depends on NFAT1 transcription factor and regulates Th differentiationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Nacional de Câncer. Divisão de Biologia Celular. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Divisão de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Divisão de Biologia Celular. Rio de Janeiro, RJ, BrasiInstituto Nacional de Câncer. Divisão de Biologia Celular. Rio de Janeiro, RJ, BrasiFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Divisão de Biologia Celular. Rio de Janeiro, RJ, BrasiCD8+ T lymphocytes are excellent sources of IFN-gamma; however, the molecular mechanisms that dictate IFN-gamma expression upon TCR stimulation in these cells are not completely understood. In this study, we evaluated the involvement of NFAT1 in the regulation of IFN-gamma gene expression in murine CD8+ T cells and its relevance during Th differentiation. We show that CD8+, but not CD4+, T cells, represent the very first source of IFN-gamma upon primary T cell activation, and also that the IFN-gamma produced by naive CD8+ T cells may enhance CD4+ Th1 differentiation in vitro. TCR stimulation rapidly induced IFN-gamma expression in CD8+ T lymphocytes in a cyclosporin A-sensitive manner. Evaluation of CD8+ T cells showed that calcium influx alone was sufficient to activate NFAT1 protein, transactivate IFN-gamma gene promoter, and induce IFN-gamma production. In fact, NFAT1-deficient mice demonstrated highly impaired IFN-gamma production by naive CD8+ T lymphocytes, which were totally rescued after retroviral transduction with NFAT1-encoding vectors. Moreover, NFAT1-dependent IFN-gamma production by the CD8+ T cell compartment was crucial to control a Th2-related response in vivo, such as allergic inflammation. Consistently, CD8alpha- as well as IFN-gamma-deficient mice did not mount a Th1 immune response and also developed in vivo allergic inflammation. Our results clearly indicate that IFN-gamma production by CD8+ T cells is dependent of NFAT1 transcription factor and may be an essential regulator of Th immune responses in vivo.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/40903/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLeonardoTeixeira_PatriciaTBozza_etal_IOC_2005.pdfLeonardoTeixeira_PatriciaTBozza_etal_IOC_2005.pdfapplication/pdf796314https://www.arca.fiocruz.br/bitstream/icict/40903/2/LeonardoTeixeira_PatriciaTBozza_etal_IOC_2005.pdf7b8be44298f89708461474ceefeb96deMD52TEXTLeonardoTeixeira_PatriciaTBozza_etal_IOC_2005.pdf.txtLeonardoTeixeira_PatriciaTBozza_etal_IOC_2005.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv IFN-gamma production by CD8+ T cells depends on NFAT1 transcription factor and regulates Th differentiation
title IFN-gamma production by CD8+ T cells depends on NFAT1 transcription factor and regulates Th differentiation
spellingShingle IFN-gamma production by CD8+ T cells depends on NFAT1 transcription factor and regulates Th differentiation
Teixeira, Leonardo K.
CD8 T Cells
IFN-y
LInfócitos T
CD8 T Cells
IFN-y Production
T lymphocytes
title_short IFN-gamma production by CD8+ T cells depends on NFAT1 transcription factor and regulates Th differentiation
title_full IFN-gamma production by CD8+ T cells depends on NFAT1 transcription factor and regulates Th differentiation
title_fullStr IFN-gamma production by CD8+ T cells depends on NFAT1 transcription factor and regulates Th differentiation
title_full_unstemmed IFN-gamma production by CD8+ T cells depends on NFAT1 transcription factor and regulates Th differentiation
title_sort IFN-gamma production by CD8+ T cells depends on NFAT1 transcription factor and regulates Th differentiation
author Teixeira, Leonardo K.
author_facet Teixeira, Leonardo K.
Fonseca, Bruna P. F.
Abreu, Adriana Vieira de
Barboza, Bianca A.
Robbs, Bruno K.
Bozza, Patrícia T.
Viola, João P. B.
author_role author
author2 Fonseca, Bruna P. F.
Abreu, Adriana Vieira de
Barboza, Bianca A.
Robbs, Bruno K.
Bozza, Patrícia T.
Viola, João P. B.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Teixeira, Leonardo K.
Fonseca, Bruna P. F.
Abreu, Adriana Vieira de
Barboza, Bianca A.
Robbs, Bruno K.
Bozza, Patrícia T.
Viola, João P. B.
dc.subject.other.pt_BR.fl_str_mv CD8 T Cells
IFN-y
LInfócitos T
topic CD8 T Cells
IFN-y
LInfócitos T
CD8 T Cells
IFN-y Production
T lymphocytes
dc.subject.en.pt_BR.fl_str_mv CD8 T Cells
IFN-y Production
T lymphocytes
description Instituto Nacional de Câncer. Divisão de Biologia Celular. Rio de Janeiro, RJ, Brasil.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2020-04-21T14:09:41Z
dc.date.available.fl_str_mv 2020-04-21T14:09:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TEIXEIRA, Leonardo K. et al. IFN- Production by CD8 T Cells Depends on NFAT1 Transcription Factor and Regulates Th Differentiation. Journal of Immunology, v. 175, p. 5931-5939, 2005.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/40903
dc.identifier.issn.pt_BR.fl_str_mv 0022-1767
dc.identifier.doi.none.fl_str_mv 10.4049/jimmunol.175.9.5931
dc.identifier.eissn.none.fl_str_mv 1550-6606
identifier_str_mv TEIXEIRA, Leonardo K. et al. IFN- Production by CD8 T Cells Depends on NFAT1 Transcription Factor and Regulates Th Differentiation. Journal of Immunology, v. 175, p. 5931-5939, 2005.
0022-1767
10.4049/jimmunol.175.9.5931
1550-6606
url https://www.arca.fiocruz.br/handle/icict/40903
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Association of Immunologists
publisher.none.fl_str_mv American Association of Immunologists
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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