Role of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammation

Detalhes bibliográficos
Autor(a) principal: Alberto, Anael Viana Pinto
Data de Publicação: 2016
Outros Autores: Faria, Robson Xavier, Menezes, João Ricardo Lacerda de, Surrage, Andrea, Rocha, Natasha Cristina da, Ferreira, Leonardo Gomes Braga, Frutuoso, Valber da Silva, Martins, Marco Aurélio, Nunes, Luiz Anastácio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14235
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.
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spelling Alberto, Anael Viana PintoFaria, Robson XavierMenezes, João Ricardo Lacerda deSurrage, AndreaRocha, Natasha Cristina daFerreira, Leonardo Gomes BragaFrutuoso, Valber da SilvaMartins, Marco AurélioNunes, Luiz Anastácio2016-05-11T13:05:03Z2016-05-11T13:05:03Z2016ALBERTO, Anael Viana Pinto; et al. Role of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammation. Plos One, v.11, n.1, e0145392, 17p, Jan. 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/1423510.1371/journal. pone.0145392engPublic Library of ScienceEosinófilosInflamação alérgicaEosinófilos de ratoP2 ReceptoresP2 ReceptorsAllergic InflammationRat eosinophilsRole of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Laboratório de Neuroanatomia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.ATP and other nucleotides are released from cells through regulated pathways or following the loss of plasma membrane integrity. Once outside the cell, these compounds can activate P2 receptors: P2X ionotropic receptors and G protein-coupled P2Y receptors. Eosinophils represent major effector cells in the allergic inflammatory response and they are, in fact, associated with several physiological and pathological processes. Here we investigate the expression of P2 receptors and roles of those receptors in murine eosinophils. In this context, our first step was to investigate the expression and functionality of the P2X receptors by patch clamping, our results showed a potency ranking order of ATP>ATPγS> 2meSATP> ADP> αβmeATP> βγmeATP>BzATP> UTP> UDP>cAMP. This data suggest the presence of P2X1, P2X2 and P2X7. Next we evaluate by microfluorimetry the expression of P2Y receptors, our results based in the ranking order of potency (UTP>ATPγS> ATP > UDP> ADP >2meSATP > αβmeATP) suggests the presence of P2Y2, P2Y4, P2Y6 and P2Y11. Moreover, we confirmed our findings by immunofluorescence assays. We also did chemotaxis assays to verify whether nucleotides could induce migration. After 1 or 2 hours of incubation, ATP increased migration of eosinophils, as well as ATPγS, a less hydrolysable analogue of ATP, while suramin a P2 blocker abolished migration. In keeping with this idea, we tested whether these receptors are implicated in the migration of eosinophils to an inflammation site in vivo, using a model of rat allergic pleurisy. In fact, migration of eosinophils has increased when ATP or ATPγS were applied in the pleural cavity, and once more suramin blocked this effect. We have demonstrated that rat eosinophils express P2X and P2Y receptors. In addition, the activation of P2 receptors can increase migration of eosinophils in vitro and in vivo, an effect blocked by suramin.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14235/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALanael_alberto_etal_IOC_2016.PDFanael_alberto_etal_IOC_2016.PDFapplication/pdf4175512https://www.arca.fiocruz.br/bitstream/icict/14235/2/anael_alberto_etal_IOC_2016.PDF22f959d1a22fd35be10b0d7ac7a17e28MD52TEXTanael_alberto_etal_IOC_2016.PDF.txtanael_alberto_etal_IOC_2016.PDF.txtExtracted texttext/plain45992https://www.arca.fiocruz.br/bitstream/icict/14235/3/anael_alberto_etal_IOC_2016.PDF.txt3a7329a3015f67948b7b93c61525c582MD53icict/142352018-04-02 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dc.title.pt_BR.fl_str_mv Role of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammation
title Role of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammation
spellingShingle Role of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammation
Alberto, Anael Viana Pinto
Eosinófilos
Inflamação alérgica
Eosinófilos de rato
P2 Receptores
P2 Receptors
Allergic Inflammation
Rat eosinophils
title_short Role of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammation
title_full Role of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammation
title_fullStr Role of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammation
title_full_unstemmed Role of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammation
title_sort Role of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammation
author Alberto, Anael Viana Pinto
author_facet Alberto, Anael Viana Pinto
Faria, Robson Xavier
Menezes, João Ricardo Lacerda de
Surrage, Andrea
Rocha, Natasha Cristina da
Ferreira, Leonardo Gomes Braga
Frutuoso, Valber da Silva
Martins, Marco Aurélio
Nunes, Luiz Anastácio
author_role author
author2 Faria, Robson Xavier
Menezes, João Ricardo Lacerda de
Surrage, Andrea
Rocha, Natasha Cristina da
Ferreira, Leonardo Gomes Braga
Frutuoso, Valber da Silva
Martins, Marco Aurélio
Nunes, Luiz Anastácio
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alberto, Anael Viana Pinto
Faria, Robson Xavier
Menezes, João Ricardo Lacerda de
Surrage, Andrea
Rocha, Natasha Cristina da
Ferreira, Leonardo Gomes Braga
Frutuoso, Valber da Silva
Martins, Marco Aurélio
Nunes, Luiz Anastácio
dc.subject.other.pt_BR.fl_str_mv Eosinófilos
Inflamação alérgica
Eosinófilos de rato
P2 Receptores
topic Eosinófilos
Inflamação alérgica
Eosinófilos de rato
P2 Receptores
P2 Receptors
Allergic Inflammation
Rat eosinophils
dc.subject.en.pt_BR.fl_str_mv P2 Receptors
Allergic Inflammation
Rat eosinophils
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-05-11T13:05:03Z
dc.date.available.fl_str_mv 2016-05-11T13:05:03Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALBERTO, Anael Viana Pinto; et al. Role of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammation. Plos One, v.11, n.1, e0145392, 17p, Jan. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14235
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal. pone.0145392
identifier_str_mv ALBERTO, Anael Viana Pinto; et al. Role of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammation. Plos One, v.11, n.1, e0145392, 17p, Jan. 2016.
1932-6203
10.1371/journal. pone.0145392
url https://www.arca.fiocruz.br/handle/icict/14235
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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